A highly efficient method for the generation of a recombinant Bombyx mori nuclear-polyhedrosis-virus Bacmid and large-scale expression of foreign proteins in silkworm (B. mori) larvae

Biotechnology and Applied Biochemistry

Volumn 48, Issue 1, 2007, Pages 45-53

  Lun, Guang Yao ^a   Zong, Cai Liu ^a   Xiang, Man Zhang ^a   Yun, Chao Kan ^a   Zhou, Jing Jiang ^a,b  

^a NANYANG NORMAL UNIVERSITY   (China)

^b ROTHAMSTED RESEARCH   (United Kingdom)

Author keywords

Baculovirus cDNA library; Bombyx mori nuclear polyhedrosis virus (BmNPV) Bacmid; Mating assisted genetically integrated cloning (MAGIC); Red fluorescent protein tracing; Silkworm (Bombyx mori) larvae

Indexed keywords

BACULOVIRUS CDNA LIBRARY; BOMBYX MORI NUCLEAR POLYHEDROSIS VIRUS (BMNPV)-BACMID; MATING-ASSISTED GENETICALLY INTEGRATED CLONING (MAGIC); RED FLUORESCENT PROTEIN TRACING; SILKWORM (BOMBYX MORI) LARVAE;

BIODIVERSITY; CLONING; DNA; ESCHERICHIA COLI; PROTEINS; VIRUSES;

GENE EXPRESSION;

BETA MANNOSIDASE; CALCIUM PHOSPHATE; COMPLEMENTARY DNA; DNA; ENHANCED GREEN FLUORESCENT PROTEIN; PROTEIN BMBACMID; RECOMBINANT PROTEIN; RED FLUORESCENT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; BACULOVIRUS EXPRESSION SYSTEM; DNA LIBRARY; MOLECULAR CLONING; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SILKWORM; ANIMAL; GENE VECTOR; GENETIC ENHANCEMENT; GENETIC TRANSFECTION; GENETICS; LARVA; METABOLISM; METHODOLOGY; PHYSIOLOGY; POLYHEDROSIS VIRUS; PROTEIN ENGINEERING; VIROLOGY;

BOMBYX MORI; BOMBYX MORI NPV; DISCOSOMA SP.; ESCHERICHIA COLI; EUKARYOTA;

ANIMALS; BOMBYX; GENETIC ENHANCEMENT; GENETIC VECTORS; LARVA; NUCLEOPOLYHEDROVIRUS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 34548536548     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20070017     Document Type: Article

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