Detection of apoptosis in vivo: Comparison of different methods in histological sections of subcutaneous xenografts of HT29 human colon adenocarcinoma;Quantification de l'apoptose in vivo : comparaison de méthodes de détection sur coupes histologiques de tumeurs xénogreffées issus de la lignée cellulaire humaine d'adénocarcinome colique HT-29

Annales de Pathologie

Volumn 29, Issue 5, 2009, Pages 370-375

  Bressenot, Aude ^a   Zimmer, Olivia ^a   Marchal, Sophie ^b   Gauchotte, Guillaume ^a   Montagne, Karine ^a   Plénat, François ^a  

^a CHU NANCY BRABOIS   (France)

^b CENTRE DE RECHERCHE EN AUTOMATIQUE DE NANCY   (France)

Author keywords

Activated caspase 7; Actived caspase 3; Apoptosis; Cleaved cytokeratin 18; Cleaved PARP 1; Immunohistochemistry

Indexed keywords

CASPASE 3; CASPASE 7; CYTOKERATIN 18; CYTOKERATIN 19; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE 1;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL COUNT; CELL DEATH; CELL STRAIN HT29; CELL STRUCTURE; COLON ADENOCARCINOMA; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; HISTOPATHOLOGY; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; INTERMETHOD COMPARISON; MOUSE; NICK END LABELING; NONHUMAN; PHOTODYNAMIC THERAPY; PROTEIN CLEAVAGE; QUANTITATIVE ANALYSIS; RELIABILITY; SENSITIVITY ANALYSIS; TISSUE SECTION; TUMOR XENOGRAFT;

ADENOCARCINOMA; APOPTOSIS; COLONIC NEOPLASMS; HT29 CELLS; HUMANS; PATHOLOGY;

EID: 71249116006     PISSN: 02426498     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.annpat.2009.06.001     Document Type: Article

References (16)

1. Mow B.M., Blajeski A.L., Chandra J., and Kaufmann S.H. Apoptosis and the response to anticancer therapy. Curr Opin Oncol 13 (2001) 453-462
2. Fadeel B., Orrenius S., and Zhivotovsky B. The most unkindest cut of all: on the multiple roles of mammalian caspases. Leukemia 14 (2000) 1514-1525
3. Boaright M.M., and Salvesen G.S. Mechanisms of caspase activation. Curr Opin Cell Biol 15 (2003) 725-731
4. Duan W.R., Garner D.S., Williams S.D., Funckes-Shippy C.L., Spath I.S., and Blomme E.A. Comparison of immunohistochemistry for activated caspase-3 and cleaved cytokeratin 18 with the TUNEL method for quantification of apoptosis in histological sections of PC-3 subcutaneous xenografts. J Pathol 199 (2003) 221-228
5. Holubec H., Payne C.M., Bernstein H., Dvorakova K., Bernstein C., Waltmire C.N., et al. Assessment of apoptosis by immunohistochemical markers compared to cellular morphology in ex vivo-stressed colonic mucosa. J Histochem Cytochem 53 (2005) 229-235
6. Gown A.M., and Willingham M.C. Improved detection of apoptotic cells in archival paraffin sections: immunohistochemistry using antibodies to cleaved caspase 3. J Histochem Cytochem 50 (2002) 449-454
7. Vaculova A., and Zhivotovsky B. Chapter eight caspases: determination of their activities in apoptotis cells. Methods Enzymol 442 (2008) 157-181
8. Puig B., Tortosa A., and Ferrer I. Cleaved caspase-3, caspase-7 and poly (ADP-ribose) polymerase are complementarily but differentially expressed in human medulloblastomas. Neurosci Lett 306 (2001) 85-88
9. Koornstra J.J., Rijcken F.E., De Jong S., Hollema H., De Vries E.G., and Kleibeuker J.H. Assessment of apoptosis by M30 immunoreactivity and the correlation with morphological criteria in normal colorectal mucosa, adenomas and carcinomas. Histopathology 44 (2004) 9-17
10. Marchal S., Fadloun A., Maugain E., D'Hallewin M.A., Guillemin F., and Bezdetnaya L. Necrotic and apoptotic features of cell death in response to Foscan photosensitization of HT29 monolayer and multicell spheroids. Biochem Pharmacol 69 (2005) 1167-1176
11. Marchal S., Francois A., Dumas D., Guillemin F., and Bezdetnaya L. Relationship between subcellular localisation of Foscan and caspase activation in photosensitised MCF-7 cells. Br J Cancer 96 (2007) 944-951
12. Duriez P.J., and Shah G.M. Cleavage of poly(ADP-ribose) polymerase: a sensitive parameter to study cell death. Biochem Cell Biol 75 (1997) 337-349
13. Germain M., Affar E.B., D'Amours D., Dixit V.M., Salvesen G.S., and Poirier G.G. Cleavage of automodified poly(ADP-ribose) polymerase during apoptosis. Evidence for involvement of caspase-7. J Biol Chem 274 (1999) 28379-28384
14. Slee E.A., Adrain C., and Martin S.J. Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis. J Biol Chem 276 (2001) 7320-7326
15. Krajewska M., Wang H.G., Krajewski S., Zapata J.M., Shabaik A., Gascoyne R., et al. Immunohistochemical analysis of in vivo patterns of expression of CPP32 (Caspase-3), a cell death protease. Cancer Res 57 (1997) 1605-1613
16. Woo M., Hakem R., Soengas M., Duncan G., Shahinian A., Kägi D., et al. Essential contribution of caspase 3/CPP32 to apoptosis and its associated nuclear changes. F 12 (1998) 806-819