Antioxidant reactivity toward nitroxide probes anchored into human serum albumin. A new model for studying antioxidant repairing capacity of protein radicals

Bioorganic and Medicinal Chemistry Letters

Volumn 19, Issue 22, 2009, Pages 6382-6385

  Aspée, Alexis ^a   Orrego, Alejandra ^a   Alarcón, Emilio ^b   López Alarcón, Camilo ^b   Poblete, Horacio ^c   González Nilo, Danilo ^c  

^a UNIVERSIDAD DE SANTIAGO DE CHILE   (Chile)

^b PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

^c UNIVERSIDAD DE TALCA   (Chile)

Author keywords

Antioxidants; Binding constant; Human serum albumin; Nitroxide prefluorescent probes; Nitroxide reduction

Indexed keywords

ANTIOXIDANT; ASCORBIC ACID; CAFFEIC ACID; CYSTEINE; HUMAN SERUM ALBUMIN; NITROXIDE; RADICAL; THREONINE; TROLOX C;

ALBUMIN BLOOD LEVEL; AMINO ACID SUBSTITUTION; ANTIOXIDANT ACTIVITY; ARTICLE; BINDING AFFINITY; FLUORESCENCE; MOLECULAR PROBE; QUANTUM THEORY; REDUCTION;

ALBUMINS; ANAEROBIOSIS; ANTIOXIDANTS; ASCORBIC ACID; CAFFEIC ACIDS; HUMANS; NITRIC OXIDE; SERUM ALBUMIN; THERMODYNAMICS;

EID: 71049188144     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2009.09.070     Document Type: Article

References (21)

1. Richards D.M., Dean R.T., and Jessup W. Biochim. Biophys. Acta 946 (1988) 281
2. Davies M.J. Biochim. Biophys. Acta 1703 (2005) 93
3. Hawkins C.L., and Davies M.J. Biochim. Biophys. Acta 1504 (2001) 196
4. Davies M.J. Biochim. Biophys. Acta 1703 (2005) 93
5. Silvester J.A., Timmins G.S., and Davies M.J. Arch. Biochem. Biophys. 350 (1998) 249
6. Wright A., Budd W.A., Hawkins C.L., and Davies M.J. Photochem. Photobiol. 76 (2002) 35
7. Gracanin M., and Davies M.J. Free Radical Biol. Med. 42 (2007) 1543
8. Linton S., Davies M.J., and Dean R.T. Exp. Gerontol. 36 (2001) 1503
9. Morgan P.E., Dean R.T., and Davies M.J.J. Free Radical Biol. Med. 36 (2004) 484
10. Filipe P., Morliére P., Patterson L.K., Hug G.L., Maziére J.C., Maziére C., Freitas J.P., Fernandes A., and Santus R. Biochim. Biophys. Acta 1572 (2002) 150
11. Salvi A., Carrupt P.A., Tillement J.P., and Testa B. Biochem. Pharmacol. 61 (2001) 1237
12. Aliaga C., Aspée A., and Scaiano J.C. Org. Lett. 5 (2003) 4145
13. Aspée A., Aliaga C., and Scaiano J.C. Photochem. Photobiol. 83 (2007) 481
14. Aliaga C., Juárez-Ruíz J., Scaiano J.C., and Aspée A. Org. Lett. 10 (2008) 2147
15. Artarli R., Bombieri G., Calaba L., and Del Pra A. Il Fármaco 60 (2005) 485
16. Sudlow G., Birkett D.J., and Wade D.N. Mol. Pharmacol. 11 (1975) 824
17. Bueno C., Mikelsons L., Maretti L., Scaiano J.C., and Aspée A. Photochem. Photobiol. 84 (2008) 1535
18. Abuin E., Aspée A., Lissi E., and León L. J. Chil. Chem. Soc. 52 (2007) 1088
19. Abuin E., Calderón C., and Lissi E. J. Photochem. Photobiol., A. 195 (2008) 295
20. Docking simulation in HSA structure was done in AutoDock4.3 'GaussView: Version 3.09' (see Supplementary data for details).
21. obs versus the initial concentration of the antioxidant. Values for the reaction in HSA are defined as apparent rate constants when is not consider the partition of the probe in HSA.