Identification and characterization of a novel spore-associated subtilase from Thermoactinomyces sp. CDF

Microbiology

Volumn 155, Issue 11, 2009, Pages 3661-3672

  Cheng, Guyue ^a   Zhao, Peiwei ^a   Tang, Xiao Feng ^a   Tang, Bing ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

POTASSIUM CHLORIDE; PROTEINASE; SUBTILASE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL SPORE; CELL INCLUSION; CONTROLLED STUDY; ENZYME STABILITY; GENE IDENTIFICATION; IMMUNOBLOTTING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PHYLOGENY; PRIORITY JOURNAL; PROTEIN DOMAIN; SALINITY; TEMPERATURE SENSITIVITY; THERMOACTINOMYCES;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLONING, MOLECULAR; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SPORES, BACTERIAL; SUBTILISINS; THERMOACTINOMYCES;

ESCHERICHIA COLI; THERMOACTINOMYCES SP.;

EID: 70450245084     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.031336-0     Document Type: Article

References (48)

1. Bendtsen, J. D., Nielsen, H., von Heijne, G. & Brunak, S. (2004). Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340, 783-795.
2. Betzel, C., Pal, G. P. & Saenger, W. (1988). Three-dimensional structure of proteinase K at 0.15-nm resolution. Eur J Biochem 178, 155-171.
3. Boschwitz, H., Halvorson, H. O., Keynan, A. & Milner, Y. (1985). Trypsinlike enzymes from dormant and germinated spores of Bacillus cereus T and their possible involvement in germination. J Bacteriol 164, 302-309.
4. Bott, R., Ultsch, M., Kossiakoff, A., Graycar, T., Katz, B. & Power, S. (1988). The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 Å and an analysis of the structural consequences of peroxide inactivation. J Biol Chem 263, 7895-7906.
5. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248-254.
6. Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T. J., Higgins, D. G. & Thompson, J. D. (2003). Multiple sequence alignment with the CLUSTAL series of programs. Nucleic Acids Res 31, 3497-3500.
7. Comellas-Bigler, M., Maskos, K., Huber, R., Oyama, H., Oda, K. & Bode, W. (2004). 1.2 Å crystal structure of the serine carboxyl proteinase pro-kumamolisin; structure of an intact pro-subtilase. Structure 12, 1313-1323.
8. Gallagher, T., Gilliland, G., Wang, L. & Bryan, P. (1995). The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'. Structure 3, 907-914.
9. Gazenko, S. V., Reponen, T. A., Grinshpun, S. A. & Willeke, K. (1998). Analysis of airborne actinomycete spores with fluorogenic substrates. Appl Environ Microbiol 64, 4410-4415.
10. Gros, P., Kalk, K. H. & Hol, W. G. (1991). Calcium binding to thermitase. Crystallographic studies of thermitase at 0, 5, and 100 mM calcium. J Biol Chem 266, 2953-2961.
11. Hilbert, D. W. & Piggot, P. J. (2004). Compartmentalization of gene expression during Bacillus subtilis spore formation. Microbiol Mol Biol Rev 68, 234-262.
12. Horsburgh, G. J., Atrih, A. & Foster, S. J. (2003). Characterization of LytH, a differentiation-associated peptidoglycan hydrolase of Bacillus subtilis involved in endospore cortex maturation. J Bacteriol 185, 3813-3820.
13. Jacobs, M., Eliasson, M., Uhlen, M. & Flock, J. I. (1985). Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis. Nucleic Acids Res 13, 8913-8926.
14. Kaneko, R., Koyama, N., Tsai, Y. C., Juang, R. Y., Yoda, K. & Yamasaki, M. (1989). Molecular cloning of the structural gene for alkaline elastase YaB, a new subtilisin produced by an alkalophilic Bacillus strain. J Bacteriol 171, 5232-5236.
15. King, J. & Laemmli, U. K. (1971). Polypeptides of the tail fibres of bacteriophage T4. J Mol Biol 62, 465-477.
16. Kleine, R. (1982). Properties of thermitase, a thermostable serine protease from Thermoactinomyces vulgaris. Acta Biol Med Ger 41, 89-102.
17. Kojima, S., Minagawa, T. & Miura, K. (1997). The propeptide of subtilisin BPN' as a temporary inhibitor and effect of an amino acid replacement on its inhibitory activity. FEBS Lett 411, 128-132.
18. Lacey, J. (1989). Thermoactinomycetes. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2573-2585. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore, MD: Lippincott, Williams & Wilkins.
19. Lee, J. K., Kim, Y. O., Kim, H. K., Park, Y. S. & Oh, T. K. (1996). Purification and characterization of a thermostable alkaline protease from Thermoactinomyces sp. E79 and the DNA sequence of the encoding gene. Biosci Biotechnol Biochem 60, 840-846.
20. Liu, Y. G. & Whittier, R. F. (1995). Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25, 674-681.
21. Makhatadze, G. I., Loladze, V. V., Ermolenko, D. N., Chen, X. & Thomas, S. T. (2003). Contribution of surface salt bridges to protein stability: guidelines for protein engineering. J Mol Biol 327, 1135-1148.
22. Markland, F. S. & Smith, E. L. (1967). Subtilisin BPN'. VII. Isolation of cyanogen bromide peptides and the complete amino acid sequence. J Biol Chem 242, 5198-5211.
23. Masui, A., Fujiwara, N. & Imanaka, T. (1994). Stabilization and rational design of serine protease AprM under highly alkaline and high-temperature conditions. Appl Environ Microbiol 60, 3579-3584.
24. McPhalen, C. A. & James, M. N. (1988). Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-c-subtilisin Carlsberg and CI-2-subtilisin Novo. Biochemistry 27, 6582-6598.
25. Moriyama, R., Sugimoto, K., Zhang, H., Inoue, T. & Makino, S. (1998). A cysteine-dependent serine protease associated with the dormant spores of Bacillus cereus: purification of the protein and cloning of the corresponding gene. Biosci Biotechnol Biochem 62, 268-274.
26. Nilsson, M. & Renberg, I. (1990). Viable endospores of Thermoactinomyces vulgaris in lake sediments as indicators of agricultural history. Appl Environ Microbiol 56, 2025-2028.
27. Orsini, M. & Romano-Spica, V. (2001). A microwave-based method for nucleic acid isolation from environmental samples. Lett Appl Microbiol 33, 17-20.
28. Pauwels, R., Devos, M., Callens, L. & van der Straeten, M. (1978). Respiratory hazards from proteolytic enzymes. Lancet 1, 669.
29. Pepys, J., Jenkins, P. A., Festenstein, G. N., Gregory, P. H., Lacey, M. E. & Skinner, F. A. (1963). Farmer's lung: thermophilic actinomycetes as a source of "farmer's lung hay" antigen. Lancet 2, 607-611.
30. Ramarao, N. & Lereclus, D. (2005). The InhA1 metalloprotease allows spores of the B. cereus group to escape macrophages. Cell Microbiol 7, 1357-1364.
31. Roberts, R. C., Nelles, L. P., Treuhaft, M. W. & Marx, J. J. (1983). Isolation and possible relevance of Thermoactinomyces candidus proteinases in farmer's lung disease. Infect Immun 40, 553-562.
32. Sarkar, G. & Sommer, S. S. (1990). The "megaprimer" method of site-directed mutagenesis. Biotechniques 8, 404-407.
33. Schagger, H. & von Jagow, G. (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166, 368-379.
34. Schalinatus, E., Behnke, U. & Ruttloff, H. (1983a). Intracellular proteases of Thermoactinomyces vulgaris. 1. Occurrence of the proteases in the cell and dynamics of their formation during cultivation. Nahrung 27, 371-377.
35. Schalinatus, E., Ruttloff, H. & Behnke, U. (1983b). Intracellular proteases of Thermoactinomyces vulgaris. 2. Biochemical characterization of proteases in cell extracts. Nahrung 27, 379-386.
36. Siezen, R. J. & Leunissen, J. A. (1997). Subtilases: the superfamily of subtilisin-like serine proteases. Protein Sci 6, 501-523.
37. Smith, C. A., Toogood, H. S., Baker, H. M., Daniel, R. M. & Baker, E. N. (1999). Calcium-mediated thermostability in the subtilisin superfamily: the crystal structure of Bacillus Ak.1 protease at 1.8 Å resolution. J Mol Biol 294, 1027-1040.
38. Tanaka, S.-I., Matsumura, H., Koga, Y., Takano, K. & Kanaya, S. (2007). Four new crystal structures of Tk-subtilisin in unautoprocessed, autoprocessed and mature forms: insight into structural changes during maturation. J Mol Biol 372, 1055-1069.
39. Teplyakov, A. V., Kuranova, I. P., Harutyunyan, E. H., Vainshtein, B. K., Frommel, C., Hohne, W. E. & Wilson, K. S. (1990). Crystal structure of thermitase at 1.4 Å resolution. J Mol Biol 214, 261-279.
40. Tesone, C. & Torriani, A. (1975). Protease associated with spores of Bacillus cereus. J Bacteriol 124, 593-594.
41. Tsuchiya, K., Nakamura, Y., Sakashita, H. & Kimura, T. (1992). Purification and characterization of a thermostable alkaline protease from alkalophilic Thermoactinomyces sp. HS682. Biosci Biotechnol Biochem 56, 246-250.
42. Tsuchiya, K., Ikeda, I., Tsuchiya, T. & Kimura, T. (1997). Cloning and expression of an intracellular alkaline protease gene from alkalophilic Thermoactinomyces sp. HS682. Biosci Biotechnol Biochem 61, 298-303.
43. Wong, S. L., Price, C. W., Goldfarb, D. S. & Doi, R. H. (1984). The subtilisin E gene of Bacillus subtilis is transcribed from a sigma 37 promoter in vivo. Proc Natl Acad Sci U S A 81, 1184-1188.
44. Wu, J., Bian, Y., Tang, B., Chen, X., Shen, P. & Peng, Z. (2004). Cloning and analysis of WF146 protease, a novel thermophilic subtilisin-like protease with four inserted surface loops. FEMS Microbiol Lett 230, 251-258.
45. Yabuta, Y., Takagi, H., Inouye, M. & Shinde, U. (2001). Folding pathway mediated by an intramolecular chaperone: propeptide release modulates activation precision of pro-subtilisin. J Biol Chem 276, 44427-44434.
46. Yamagata, Y., Isshiki, K. & Ichishima, E. (1995). Subtilisin Sendai from alkalophilic Bacillus sp.: molecular and enzymatic properties of the enzyme and molecular cloning and characterization of the gene, aprS. Enzyme Microb Technol 17, 653-663.
47. Yike, I., Rand, T. & Dearborn, D. G. (2007). The role of fungal proteinases in pathophysiology of Stachybotrys chartarum. Mycopathologia 164, 171-181.
48. Yoon, J.-H., Kim, I.-G., Shin, Y.-K. & Park, Y.-H. (2005). Proposal of the genus Thermoactinomyces sensu stricto and three new genera, Laceyella, Thermoflavimicrobium and Seinonella, on the basis of phenotypic, phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 55, 395-400.