"Reverse degradomics", monitoring of proteolytic trimming by multi-CE and confocal detection of fluorescent substrates and reaction products

Electrophoresis

Volumn 30, Issue 13, 2009, Pages 2366-2377

  Piccard, Helene ^a   Hu, Jialiang ^a   Fiten, Pierre ^a   Proost, Paul ^a   Martens, Erik ^a   Van Den Steen, Philippe E ^a   Van Damme, Jo ^a   Opdenakker, Ghislain ^a  

^a REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

Author keywords

Enzymatic peptide labeling; Laser induced confocal detection; Multicapillary electrophoresis; Post translational modification; Proteolytic processing

Indexed keywords

CELL CULTURE; DATA HANDLING; DIAGNOSIS; ELECTROPHORESIS; FLUORESCENCE; GLYCOSYLATION; PROTEOLYSIS; REACTION PRODUCTS; SUBSTRATES;

CONFOCAL DETECTIONS; ENZYMATIC PEPTIDE LABELING; LABELINGS; LASER INDUCED; LASER-INDUCED CONFOCAL DETECTION; MULTICAPILLARIES; MULTICAPILLARY ELECTROPHORESIS; POST-TRANSLATIONAL MODIFICATIONS; PROTEOLYTIC ACTIVITIES; PROTEOLYTIC PROCESSING;

PEPTIDES;

AMINO ACID; FLUORESCENT DYE; GRANULOCYTE CHEMOTACTIC PROTEIN 2; MONOCLONAL ANTIBODY; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; PROTEINASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOLOGICAL ACTIVITY; CAPILLARY ELECTROPHORESIS; CHEMICAL INTERACTION; CHEMICAL REACTION; CLASSIFICATION; COMPLEX FORMATION; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME SUBSTRATE; HIGH THROUGHPUT SCREENING; MOUSE; NONHUMAN; PEPTIDE SYNTHESIS; PROTEIN DEGRADATION; PROTEIN PROCESSING;

ANIMALS; ANTIBODIES, MONOCLONAL; CHEMOKINE CXCL6; ELECTROPHORESIS, CAPILLARY; FLUORESCENT DYES; MATRIX METALLOPROTEINASE 9; MICE; MICROSCOPY, CONFOCAL; PEPTIDE HYDROLASES; PEPTIDE LIBRARY; PEPTIDES; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, TERTIARY; PROTEOMICS; SENSITIVITY AND SPECIFICITY; TRANSGLUTAMINASES;

EID: 70450216507     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200800698     Document Type: Article

References (28)

1. López-Otín, C., Matrisian, L. M., Nat.Rev.Cancer. 2007, 7, 800-808.
2. Pritchard, L. E., White, A., Endocrinology. 2007, 148, 4201-4207.
3. Moreau, M. E., Garbacki, N., Molinaro, G., Brown, N. J., Marceau, F., Adam, A., J. Pharmacol. Sci. 2005, 99, 6-38.
4. McQuibban, G. A., Gong, J. H., Tam, E. M., McCulloch, C. A., Clark-Lewis, I., Overall, C. M., Science. 2000, 289, 1202-1206.
5. Van den Steen, P. E., Proost, P., Wuyts, A., Van Damme, J., Opdenakker, G., Blood. 2000, 96, 2573-2581.
6. López-Otín, C., Overall, C. M., Nat. Rev. Mol. Cell Biol. 2002, 3, 509-519.
7. Vierstraete, E., Verleyen, P., Baggerman, G., D'Hertog,W., Van den Bergh, G., Arckens, L., De Loof, A., Proc. Natl. Acad. Sci. USA 2004, 101, 470-475.
8. Van Damme, P., Martens, L., Van Damme, J., Hugelier, K., Staes, A., Vandekerckhove, J., Gevaert, K., Nat. Methods. 2005, 2, 771-777.
9. Tam, E. M., Morrison, C. J., Wu, Y. I., Stack, M. S., Overall, C. M., Proc. Natl. Acad. Sci. USA 2004, 101, 6917-6922.
10. Schilling, O., Overall, C. M., Curr. Opin. Chem. Biol. 2007, 11, 36-45.
11. Overall, C. M., Blobel, C. P., Nat. Rev. Mol. Cell Biol. 2007, 8, 245-257.
12. Greenlee, K. J., Corry, D. B., Engler, D. A.,Matsunami, R. K., Tessier, P., Cook, R. G., Werb, Z. J. Immunol. 2006, 177, 7312-7321.
13. Hu, J., Van den Steen, P. E., Sang, Q. X., Opdenakker, G., Nat. Rev. Drug Discov. 2007, 6, 480-498.
14. Wuyts, A., Haelens, A., Opdenakker, G., Proost, P., Van Damme, J., J. Immunol. 1996, 157, 1736-1743.
15. Wuyts, A., D'Haese, A., Cremers, V., Menten, P., Lenaerts, J. P., De Loof, A., Heremans, H., J. Immunol. 1999, 163, 6155-6163.
16. Hu, J., Fiten, P., Van den Steen, P. E., Chaltin, P., Opdenakker, G., Anal. Chem. 2005, 77, 2116-2124.
17. Huston, J. S., Levinson, D., Mudgett-Hunter, M., Tai, M. S., Novotny, J., Margolies, M. N., Ridge, R. J. et al., Proc. Natl. Acad. Sci. USA 1988, 85, 5879-5883.
18. Lam, K. S., Salmon, S. E., Hersh, E. M., Hruby, V. J., Kazmierski, W. M., Knapp, R. J., Nature. 1991, 354, 82-84.
19. Li, X. H., Paulus, G., Atassi, G., Buyssens, N., Am. J. Pathol. 1984, 115, 403-411.
20. Bradford, M. M., Anal. Biochem. 1976, 72, 248-254.
21. Richard, M., Grencis, R. K., Humphreys, N. E., Renauld, J. C., Van Snick, J., Proc. Natl. Acad. Sci. USA 2000, 97, 767-772.
22. Pastor, M. T., Diez, A., Perez-Paya, E., Abad, C., FEBS Lett. 1999, 451, 231-234.
23. Folk, J. E., Cole, P. W., J. Biol. Chem. 1965, 240, 2951-2960.
24. Coussons, P. J., Price, N. C., Kelly, S. M., Smith, B., Sawyer, L., et al., BiochemJ. 1992, 282, 929-930.
25. Opdenakker, G., Van Damme, J., Int. J. Dev. Biol. 2004, 48, 519-527.
26. Opdenakker, G., Van den Steen, P. E., Laureys, G., Hunninck, K., Arnold, B., Trends Immunol. 2003, 24, 94-100.
27. Hu, J., Van den Steen, P. E., Dillen, C., Opdenakker, G., Biochem. Pharmacol. 2005, 70, 535-544.
28. Fonovic, M., Bogyo, M., Curr. Pharm. Des. 2007, 13, 253-261.