Immuno-capture as ultimate sample cleanup in LC-MS/MS determination of the early stage biomarker ProGRP

Journal of Separation Science

Volumn 32, Issue 17, 2009, Pages 2937-2943

  Winther, Bjørn ^a   Nordlund, Marianne ^b   Paus, Elisabeth ^b   Reubsaet, Léon ^a   Halvorsen, Trine Grønhaug ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

Immuno capture; LC MS MS; ProGRP; Signature peptide; Tryptic digestion

Indexed keywords

HUMAN SERUM; IMMUNO-CAPTURE; LC-MS METHODS; LC-MS/MS; METHOD EVALUATION; MICROTITER PLATES; PROTEIN PRECIPITATION; QUADROPOLE; REFERENCE METHOD; SAMPLE CLEAN-UP; SAMPLE PREPARATION; SAMPLE PREPARATION PROCEDURE; SMALL-CELL LUNG CANCER; SPE CLEAN-UP; TRYPTIC DIGESTION;

BODY FLUIDS; PEPTIDES; SOLAR RADIATION;

AMINES;

BIOLOGICAL MARKER; PROGASTRIN RELEASING PEPTIDE; TRYPSIN;

ARTICLE; DILUTION; ELUTION; HUMAN; IMMUNO CAPTURE; IMMUNOASSAY; IMMUNOCHEMISTRY; LIQUID CHROMATOGRAPHY; LUNG SMALL CELL CANCER; MASS SPECTROMETRY; PRECIPITATION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN BLOOD LEVEL; PROTEIN DEGRADATION; PROTEIN DETERMINATION; SENSITIVITY ANALYSIS;

BIOLOGICAL MARKERS; CHROMATOGRAPHY, LIQUID; HUMANS; IMMUNOASSAY; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SMALL CELL LUNG CARCINOMA; TANDEM MASS SPECTROMETRY;

EID: 70450179059     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.200900233     Document Type: Article

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