Simultaneous measurement of WSpH and overloaded band profiles of small peptides when insufficiently buffered mobile phases are used in preparative liquid chromatography

Journal of Chromatography A

Volumn 1216, Issue 51, 2009, Pages 8874-8882

  Gritti, Fabrice ^a   Guiochon, Georges ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

WSpH; Adsorption mechanism; Buffer adsorption; Ionic strength; Methanol water mobile phases; Multi pKa compounds; Peptide analysis; Peptide purification; Phenylalanine alanine; Preparative HPLC of acido basic compounds; RPLC; System peaks; XBridge C18

Indexed keywords

ADSORPTION MECHANISM; BASIC COMPOUNDS; BUFFER ADSORPTION; METHANOL-WATER; MOBILE PHASIS; PEPTIDE ANALYSIS; PREPARATIVE HPLC; SYSTEM PEAKS;

ADSORPTION; ADSORPTION ISOTHERMS; AMINES; AMINO ACIDS; CHROMATOGRAPHIC ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; IONIC STRENGTH; IONIZATION OF LIQUIDS; METHANOL; PEPTIDES; POROUS MATERIALS; PURIFICATION; SILICA;

DEWATERING;

ACETIC ACID; ALANINE; AMMONIA; AMPHOLYTE; BUFFER; CARBON; CARBONIC ACID; FORMIC ACID; METHANOL; PHENYLALANINE; PHOSPHATE; SILICON DIOXIDE; WATER;

ACIDITY; ADSORPTION; ARTICLE; CHEMICAL STRUCTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ELUTION; IONIC STRENGTH; ISOTHERM; LIQUID CHROMATOGRAPHY; PARTICLE SIZE; PEPTIDE ANALYSIS; PH; POROSITY; PRIORITY JOURNAL; REVERSED PHASE LIQUID CHROMATOGRAPHY; SAMPLE SIZE; SURFACE PROPERTY;

BUFFERS; CHROMATOGRAPHY, LIQUID; DIPEPTIDES; HYDROGEN-ION CONCENTRATION; OSMOLAR CONCENTRATION; SOLUTIONS; SOLVENTS;

EID: 70449753865     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2009.10.026     Document Type: Article

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