Isolation and characterization of a cDNA encoding a plant defensin-like protein from roots of Norway spruce

Plant Molecular Biology

Volumn 31, Issue 3, 1996, Pages 707-712

  Sharma, Praveen ^a   Lönneborg, Anders ^a  

^a NORWEGIAN INSTITUTE OF BIOECONOMY RESEARCH   (Norway)

Author keywords

cDNA clone; defence response; Picea abies; plant defensin; Pythium dimorphum; roots; thionin

Indexed keywords

ABIES; FUNGI; PICEA; PICEA ABIES; PYTHIUM DIMORPHUM;

EID: 0030175226     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00042244     Document Type: Article

References (42)

1. Andresen I, Becker W, Schlüter K, Burges J, Parthier B, Apel K: The identification of leaf thionin as one of the main jasmonate-induced proteins of barley (Hordeum vulgare). Plant Mol Biol 19: 193-204 (1992).
2. Apel K, Bohlmann H, Reimann-Philip U: Leaf thionins: a novel class of putative defence factors. Physiol Plant 80: 315-321 (1990).
3. Bloch C, Richardson M: A new family of small (5 kDa) protein inhibitors of insect α-amylases from seeds or sorghum (Sorghum bicolor (L.) Moench) have sequence homologies with wheat γ-purothionins. FEBS Lett 279: 101-104 (1991).
4. Bohlmann H, Apel K: Thionins. Annu Rev Plant Physiol Plant Mol Biol 42: 227-240 (1991).
5. Bohlmann H, Clausen S, Behnke S, Giese H, Hiller C, Reimann-Phillip U, Schrader G, Barkholt V, Apel K: Leaf-specific thionins of barley: a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants. EMBO J 7: 1559-1565 (1988).
6. Børja I, Sharma P, Krekling T, Lönneborg A: Cytopathological response in roots of Picea abies seedlings infected with Pythium dimorphum. Phytopathology 85: 495-501 (1995).
7. Bowles DJ: Defense-related proteins in higher plants. Annu Rev Biochem 59: 873-907 (1990).
8. 1H-NMR: a structural motif common to toxic arthropod proteins. Biochemistry 32: 715-724 (1993).
9. Carmona MJ, Molina A, Fernandez JA, Lopez-Fando JJ, Garcia-Olmedo F: Expression of the α-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens. Plant J 3: 457-462 (1993).
10. Carrasco L, Vásquez D, Hernández-Lucas C, Carbonero P, Garcia-Olmedo F: Thionins, plant peptides that modify membrane permeability in cultured mammalian cells. Eur J Biochem 116: 185-189 (1981).
11. Colilla FJ, Rocher A, Mendez E. γ-purothionins: amino acid sequences of two polypeptides of a new family of thionins from wheat endosperm. FEBS Lett 270: 191-194 (1990).
12. Collinge DB, Slusarenko AJ: Plant gene expression in response to pathogens. Plant Mol Biol 9: 389-410 (1987).
13. Cramer CL, Ryder TB, Bell JN, Lamb CJ: Rapid switching of plant gene expression induced by fungal elicitor. Science 227: 1240-1243 (1985).
14. Dalkin K, Jorrin J, Dixon RA: Stress responses in alfalfa (Medicago sativa L.). VII. Induction of defense related mRNAs in elicitor-treated cell suspension cultures. Physiol Mol Plant Path 37: 293-307 (1990).
15. Dixon RA, Lamb CJ: Molecular communication in interactions between plants and microbial pathogens. Annu Rev Plant Physiol Plant Mol Biol 41: 339-367 (1990).
16. Ebrahim-Nesbat F, Behnke S, Kleinhofs A, Apel K: Cultivar-related differences in the distribution of cell-wall-bound thionins in compatible and incompatible interactions between barley and powdery mildew. Planta 179: 203-210 (1989).
17. Florack DEA, Stiekema WJ: Thionins: properties, possible biological roles and mechanisms of action. Plant Mol Biol 26: 25-37 (1994).
18. Fernandez de Caleya R, Gonzalez-Pascual B, Garcia-Olmedo F, Carbonero P: Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro. Appl Microbiol 23: 998-1000 (1972).
19. Garcia-Olmedo F, Carbonero P, Hernández-Lucas C, Paz-Ares J, Ponz F, Vicento O, Sierra JM: Inhibition of eukaryotic cell-free protein synthesis by thionins from wheat endosperms. Biochim Biophys Acta 740: 52-56 (1983).
20. Garcia-Olmedo F, Rodriguez-Palenzuela P, Hernández-Lucas C, Ponz F, Mariana C, Carmona MJ, Lopez-Fando J, Fernandez JA, Carbonero P: The thionins: a protein family that includes purothionins, viscotoxins and crambins. Oxford Surv Plant Mol Cell Biol 6: 31-60 (1989).
21. Genetics Computer Group: Program Manual for the Wisconsin package, version 8. GCG, Madison, WI (1994).
22. Good AG, Crosby WL: Induction of alcohol dehydrogenase and lactate dehydrogenase in hypoxically induced barley. Plant Physiol 90: 860-866 (1989).
23. Hake S, Kelley PM, Taylor WC, Freeling M: Coordinate induction of alcohol dehydrogenase 1, aldolase and other anaerobic RNAs in maize. J Biol Chem 260: 5050-5054 (1985).
24. Ishibashi N, Yamauchi D, Minamikawa T: Stored mRNA in cotyledons of Vigna unguiculata seeds: nucleotide sequence of cloned cDNA for a stored mRNA and induction of its synthesis by precocious germination. Plant Mol Biol 15: 59-64 (1990).
25. Karunanandaa B, Singh A, Kao T-H: Characterization of a predominantly pistil expressed gene encoding a plant γ-thionin protein of Petunia inflata. Plant Mol Biol 26: 459-464 (1994).
26. Kelley PM: Maize pyruvate decarboxylase mRNA is induced anaerobically. Plant Mol Biol 13: 213-222 (1989).
27. Logemann J, Mayer JE, Schell J, Willmitzer L: Differential expression of genes in potato tubers after wounding. Proc Natl Acad Sci USA 85: 1136-1140 (1988).
28. Lönneborg A, Sharma P, Stougaard P: Construction of subtractive cDNA library using magnetic beads and PCR. PCR Meth Appl 4: 5168-5176 (1995).
29. Lutchke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43-48 (1987).
30. Mendez E, Moreno A, Colilla F, Pelaez F, Limas GG, Mendez R, Sorano F, Salina M, de Haro C: Primary structure and inhibition of protein synthesis in eukaryotic cell-free system of a novel thionin, γ-hordothionin, from barley endosperm. Eur J Biochem 194: 553-539 (1990).
31. Rice P: Program Manual for the EGCG Package. The Sanger Centre, Hixton Hall, Cambridge (1994).
32. Sauer N, Corbin DR, Keller B, Lamb CJ: Cloning and characterization of a wound-specific hydroxyproline-rich glycoprotein in Phaseolus vulgaris. Plant Cell Environ 13: 257-266 (1990).
33. Schultz DJ, Craig R, Cox-Foster DL, Mumma RO, Medford JI: RNA isolation from recalcitrant plant tissue. Plant Mol Biol 12: 310-316 (1994).
34. Sharma P, Børja D, Stougaard P, Lönneborg A: PR-proteins accumulating in spruce roots infected with a pathogenic Pythium sp. isolate include chitinases, chitosanases and β-1,3-glucanases. Physiol Mol Plant Path 43: 57-67 (1993).
35. Sharma P, Lönneborg A, Stougaard P: PCR based construction of subtractive cDNA library using magnetic beads. Biotechniques 15: 610-611 (1993).
36. Staub U, Polivka H, Gross HJ: Two rapid microscale procedures for isolation of total RNA from leaves rich in polyphenols and polysaccharides: application for sensitive detection of grapevine viroids. J Virol Meth 52: 209-218 (1995).
37. Stiekema WJ, Heidekamp F, Dirkse WG, van Beckum J, de Haan P, ten Bosch C, Louwerse JD: Molecular cloning and analysis of four potato tuber mRNAs. Plant Mol Biol 11: 255-269 (1989).
38. Terras FRG, Eggermont K, Kovaleva V, Raikhel NV, Osborn RW, Rester A, Rees SB, Torrekens S, Leuven FV, Vanderleyden J, Cammue BPA, Broekaert WF: Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7: 573-588 (1995).
39. Titarenko E, Hargreaves J, Keon J, Gurr SJ: Defense-related gene expression in barley coleoptile cells following infection by Septoria nodorum. In: Fritig B, Legrand M (eds) Mechanisms of Plant Defense Responses, pp. 308-311. Kluwer Academic Publishers, Dordrecht (1993).
40. von Heijne G: A new method for predicting signal sequence cleavage sites. Nucl Acids Res 14: 4683-4690 (1986).
41. Xie Y, Wu R: Rice alcohol dehydrogenase genes: anaerobic induction, organ specific expression and characterization of cDNA clones. Plant Mol Biol 13: 53-68 (1989).
42. Zhang S, Sheng J, Liu Y, Mehdy MC: Fungal elicitor-induced bean proline-rich protein mRNA downregulation is due to destabilization that is transcription and translation dependent. Plant Cell 5: 1089-1099 (1993).