A visible wavelength spectrophotometric assay suitable for high-throughput screening of 3-hydroxy-3-methylglutaryl-CoA synthase

Analytical Biochemistry

Volumn 396, Issue 1, 2010, Pages 96-102

  Andrew Skaff, D ^a   Miziorko, Henry M ^a  

^a UNIVERSITY OF MISSOURI KANSAS CITY   (United States)

Author keywords

Dithiobisnitrobenzoic acid (DTNB); High throughput screening; HMG CoA synthase; Visible wavelength assay

Indexed keywords

ACETYLATION; ALCOHOLS; BIOCHEMISTRY; DRUG PRODUCTS; ENZYME ACTIVITY; LIPIDS; SPECTROPHOTOMETERS; SPECTROPHOTOMETRY; SUBSTRATES;

DITHIOBISNITROBENZOIC ACID (DTNB); HIGH THROUGHPUT SCREENING; HIGH-THROUGHPUT ASSAYS; POTENTIAL DRUG TARGETS; RECOMBINANT ENZYMES; SPECTROPHOTOMETRIC ASSAYS; SYNTHASES; VISIBLE WAVELENGTHS;

ENZYME INHIBITION;

5,5' DITHIOBIS(2 NITROBENZOIC ACID); COENZYME A; HYDROXYMETHYLGLUTARYL COENZYME A SYNTHASE; RECOMBINANT ENZYME;

ACETYLATION; ARTICLE; CONTROLLED STUDY; DRUG SCREENING; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME RELEASE; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; HIGH THROUGHPUT SCREENING; HUMAN; INTERMETHOD COMPARISON; MOLECULAR DOCKING; PRIORITY JOURNAL; PROTEIN POLYMERIZATION; RELIABILITY; SPECTROPHOTOMETRY;

EID: 70449624854     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.08.030     Document Type: Article

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