Purification of RNA polymerase from mycobacteria for optimized promoter-polymerase interactions

Protein Expression and Purification

Volumn 69, Issue 2, 2010, Pages 235-242

  China, Arnab ^a   Nagaraja, Valakunja ^a,b  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b JAWAHARLAL NEHRU CENTRE FOR ADVANCED SCIENTIFIC RESEARCH   (India)

Author keywords

In vitro transcription; Promoters; RNA polymerase; Sigma factors

Indexed keywords

DNA DIRECTED RNA POLYMERASE;

ARTICLE; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MYCOBACTERIUM SMEGMATIS; PROMOTER REGION; PROTEIN CONFORMATION;

DNA-DIRECTED RNA POLYMERASES; GENE EXPRESSION REGULATION, BACTERIAL; MYCOBACTERIUM SMEGMATIS; PROMOTER REGIONS, GENETIC; PROTEIN CONFORMATION; TRANSCRIPTION, GENETIC;

CORYNEBACTERINEAE; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS;

EID: 70449519188     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.09.022     Document Type: Article

References (21)

1. Murakami K.S., and Darst S.A. Bacterial RNA polymerases: the whole story. Curr. Opin. Struct. Biol. 13 (2003) 31-39
2. Gruber T.M., and Gross C.A. Multiple sigma subunits and the partitioning of bacterial transcription space. Annu. Rev. Microbiol. 57 (2003) 441-466
3. Helmann J.D., and DeHaseth P.L. Protein-nucleic acid interactions during open complex formation investigated by systematic alteration of the protein and DNA binding partners. Biochemistry 38 (1999) 5959-5967
4. Roe J.H., Burgess R.R., and Record Jr. M.T. Kinetics and mechanism of the interaction of Escherichia coli RNA polymerase with the lambda PR promoter. J. Mol. Biol. 176 (1984) 495-522
5. Predich M., Doukhan L., Nair G., and Smith I. Characterization of RNA polymerase and two sigma-factor genes Mycobacterium smegmatis. Mol. Microbiol. 15 (1995) 355-366
6. Gomez M., and Smith S. Determinants of mycobacterial gene expression. In: Hatfull G.F., and Jacobs Jr. W.R. (Eds). Molecular Genetics of Mycobacteria (2000), ASM Press, Washington, DC
7. Rodrigue S., Provvedi R., Jacques P.E., Gaudreau L., and Manganelli R. The sigma factors of Mycobacterium tuberculosis. FEMS Microbiol. Rev. 30 (2006) 926-941
8. Park H.D., Guinn K.M., Harrell M.I., Liao R., Voskuil M.I., Tompa M., Schoolnik G.K., and Sherman D.R. Rv3133c/dosR is a transcription factor that mediates the hypoxic response of Mycobacterium tuberculosis. Mol. Microbiol. 48 (2003) 833-843
9. Walters S.B., Dubnau E., Kolesnikova I., Laval F., Daffe M., and Smith I. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Mol. Microbiol. 60 (2006) 312-330
10. Rodrigue S., Brodeur J., Jacques P.E., Gervais A.L., Brzezinski R., and Gaudreau L. Identification of mycobacterial sigma factor binding sites by chromatin immunoprecipitation assays. J. Bacteriol. 189 (2007) 1505-1513
11. Mukherjee R., and Chatterji D. Stationary phase induced alterations in mycobacterial RNA polymerase assembly: a cue to its phenotypic resistance towards rifampicin. Biochem. Biophys. Res. Commun. 369 (2008) 899-904
12. In: Smith I., Bishai W.R., and Nagaraja V. (Eds). Control of Mycobacterial Transcription (2005), ASM Press, Washington, DC
13. Unniraman S., Chatterji M., and Nagaraja V. DNA gyrase genes in Mycobacterium tuberculosis: a single operon driven by multiple promoters. J. Bacteriol. 184 (2002) 5449-5456
14. Burgess R.R., and Jendrisak J.J. A procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. Biochemistry 14 (1975) 4634-4638
15. Triccas J.A., Parish T., Britton W.J., and Gicquel B. An inducible expression system permitting the efficient purification of a recombinant antigen from Mycobacterium smegmatis. FEMS Microbiol. Lett. 167 (1998) 151-156
16. Unniraman S., and Nagaraja V. Regulation of DNA gyrase operon in Mycobacterium smegmatis: a distinct mechanism of relaxation stimulated transcription. Genes Cells 4 (1999) 697-706
17. Chakraborty A., and Nagaraja V. Dual role for transactivator protein C in activation of mom promoter of bacteriophage Mu. J. Biol. Chem. 281 (2006) 8511-8517
18. Waagmeester A., Thompson J., and Reyrat J.M. Identifying sigma factors in Mycobacterium smegmatis by comparative genomic analysis. Trends Microbiol. 13 (2005) 505-509
19. Buttner M.J., Smith A.M., and Bibb M.J. At least three different RNA polymerase holoenzymes direct transcription of the agarase gene (dagA) of Streptomyces coelicolor A3(2). Cell 52 (1988) 599-607
20. Wiggs J.L., Gilman M.Z., and Chamberlin M.J. Heterogeneity of RNA polymerase in Bacillus subtilis: evidence for an additional sigma factor in vegetative cells. Proc. Natl. Acad. Sci. USA 78 (1981) 2762-2766
21. Snapper S.B., Melton R.E., Mustafa S., Kieser T., and Jacobs Jr. W.R. Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol. Microbiol. 4 (1990) 1911-1919