Bladder urothelial cells from patients with interstitial cystitis have an increased sensitivity to carbachol

Neurourology and Urodynamics

Volumn 28, Issue 8, 2009, Pages 1022-1027

  Gupta, Gopal N ^a   Lu, Shao Gang ^b,c   Gold, Michael S ^b,c   Chai, Toby C ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF MARYLAND DENTAL SCHOOL   (United States)

^c UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Bladder; Calcium; Interstitial cystitis; Muscarinic; Urothelial cells

Indexed keywords

CARBACHOL; MUSCARINIC RECEPTOR;

ADULT; ANIMAL CELL; ARTICLE; BLADDER BIOPSY; BLADDER UROTHELIAL CELL; CALCIUM CELL LEVEL; CLINICAL ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYSTOSCOPY; DRUG EFFICACY; DRUG POTENCY; DRUG SENSITIVITY; DRUG SPECIFICITY; EPITHELIUM CELL; EXTRACELLULAR CALCIUM; FEMALE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; INTERSTITIAL CYSTITIS; MALE; MICROFLUOROMETRY; MOUSE; NONHUMAN; PATHOPHYSIOLOGY; SIGNAL TRANSDUCTION;

ADULT; CALCIUM; CARBACHOL; CELLS, CULTURED; CHOLINERGIC AGONISTS; CYSTITIS, INTERSTITIAL; EPITHELIAL CELLS; FEMALE; HUMANS; MALE; MIDDLE AGED; URINARY BLADDER; UROTHELIUM;

EID: 70449377028     PISSN: 07332467     EISSN: 15206777     Source Type: Journal    
DOI: 10.1002/nau.20726     Document Type: Article

References (22)

1. Apodaca G, Balestreire E, Birder LA. The Uroepithelial-associated sensory web. Kidney Int 2007;72:1057-1064
2. Tyagi S, Tyagi P, Van-le S, et al. Qualitative and quantitative expression profile of muscarinic receptors in human urothelium and detrusor. J Urol 2006;176:1673-1678 (Pubitemid 44307245)
3. Kullmann FA, Artim DE, Birder LA, et al. Activation of muscarinic receptors in rat bladder sensory pathways alters reflex bladder activity. J Neurosci 2008;28:1977-1987
4. Andersson MC, Tobin G, Giglio D. Cholinergic nitric oxide release from the urinary bladder mucosa in cyclophosphamide-induced cystitis of the anaesthetized rat. Br J Pharmacol 2008;153:1438-1444
5. Kullmann FA, Artim D, Beckel J, et al. Heterogeneity of muscarinic receptor-mediated Ca2+ responses in cultured urothelial cells from rat. Am J Physiol Renal Physiol 2008;294:F971-81.
6. Gillenwater JY, Wein AJ. Summary of the National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases Workshop on Interstitial Cystitis, National Institutes of Health, Bethesda, Maryland, August 28-29,1987. J Urol 1988;140:203-206
7. Trifillis AL, Cui X, Jacobs S, et al. Culture of bladder epithelium from cystoscopic biopsies of patients with interstitial cystitis. J Urol 1995;153:243-248 (Pubitemid 24379850)
8. Ohuchi T, Yoshimura N, Tanihara H, et al. Ca2+ mobilization in nontransformed ciliary nonpigmented epithelial cells. Invest Ophthalmol Vis Sci 1992;33:1696-1705
9. Bschleipfer T, Schukowski K, Weidner W, et al. Expression and distribution of cholinergic receptors in the human urothelium. Life Sci 2007;80:2303-2307 (Pubitemid 46818274)
10. Mansfield KJ, Liu L, Mitchelson FJ, et al. Muscarinic receptor subtypes in human bladder detrusor and mucosa, studied by radioligand binding and quantitative competitive RT-PCR: Changes in ageing. Br J Pharmacol 2005;144:1089-1099 (Pubitemid 40593007)
11. Ishii M, Kurachi Y. Muscarinic acetylcholine receptors. Curr Pharm Des 2006;12:3573-3581
12. Thomas RL, Mistry R, Langmead CJ, et al. G protein coupling and signaling pathway activation by m1 muscarinic acetylcholine receptor orthosteric and allosteric agonists. J Pharmacol Exp Ther 2008;327:365-374
13. Michal P, El-Fakahany EE, Dolezal V. Muscarinic M2 receptors directly activate Gq/11 and Gs G-proteins. J Pharmacol Exp Ther 2007;320:607-614
14. Rohacs T, Thyagarajan B, Lukacs V. Phospholipase C mediated modulation of TRPV1 channels. Mol Neurobiol 2008;37:153-163
15. Birder LA, Nakamura Y, Kiss S, et al. Altered urinary bladder function in mice lacking the vanilloid receptor TRPV1. Nat Neurosci 2002;5:856-860 (Pubitemid 34976650)
16. Gupta GN, Li M, Sun Y, et al. Increased efficacy and potency of carbachol in inducing increases in intracellular calcium ([Ca2+])i and outward potassium currents (Io) in interstitial cystitis (IC) bladder urothelial cells (BUC): Evidence of persistent phenotypic alteration of culture. Abstract #117 presented at 2007 Annual AUA Meeting.
17. Keay S, Kleinberg M, Zhang CO, et al. Bladder epithelial cells from patients with interstitial cystitis produce an inhibitor of heparin-binding epidermal growth factor-like growth factor production. J Urol 2000;164:2112-2118 (Pubitemid 30835480)
18. Keay S, Zhang CO, Shoenfelt JL, et al. Decreased in vitro proliferation of bladder epithelial cells from patients with interstitial cystitis. Urology 2003;61:1278-1284 (Pubitemid 36694033)
19. Sun Y, Chen M, Lowentritt B, et al. EGF and HB-EGF modulate inward potassium current in human bladder urothelial cells from normal and interstitial cystitis patients. Am J Physiol Cell Physiol 2007;292:C106-14. (Pubitemid 46115124)
20. Sun Y, Chai TC. Up-regulation of P2X3 receptor during stretch of bladder urothelial cells from patients with interstitial cystitis. J Urol 2004;171:448-452 (Pubitemid 37549209)
21. Sun Y, Chai TC. Augmented extracellular ATP signaling in bladder urothelial cells from patients with interstitial cystitis. Am J Physiol Cell Physiol 2006;290:C27-34.
22. Sun Y, Keay S, De Deyne PG, et al. Augmented stretch activated adenosine triphosphate release from bladder uroepithelial cells in patients with interstitial cystitis. J Urol 2001;166:1951-1956 (Pubitemid 32953463)