Mitochondria-mediated tumstatin peptide-induced HepG2 cell apoptosis

International Journal of Molecular Medicine

Volumn 24, Issue 5, 2009, Pages 653-659

  Liu, Yan ^a   Li, Jihong ^a   Xu, Hui ^a   Zhang, Yuwen ^a   Liu, Yuanli ^a   Liu, Xinghan ^a  

^a HARBIN MEDICAL UNIVERSITY   (China)

Author keywords

Apoptosis; Mitochondrial pathway; Tumstatin

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ACRIDINE ORANGE; CASPASE 8; CASPASE 9; CHITIN; CYTOCHROME C; DITHIOTHREITOL; EOSIN; ETHIDIUM BROMIDE; FAS ANTIGEN; HEMATOXYLIN; PROTEIN BAX; PROTEIN BCL 2; PROTEIN BID; PROTEIN P53; SEPHADEX; TUMSTATIN;

AFFINITY CHROMATOGRAPHY; ANTINEOPLASTIC ACTIVITY; ARTICLE; CANCER CELL DESTRUCTION; CANCER INHIBITION; CANCER MORPHOLOGY; CARBOXY TERMINAL SEQUENCE; CELL PROLIFERATION; CELL STRAIN HEPG2; CELLULAR DISTRIBUTION; COLORIMETRY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOPLASM; DOWN REGULATION; DRUG EFFECT; DRUG MECHANISM; DRUG PURIFICATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; STAINING; STRUCTURE ACTIVITY RELATION; UPREGULATION; WESTERN BLOTTING;

ANTIGENS, CD95; APOPTOSIS; AUTOANTIGENS; BCL-2 HOMOLOGOUS ANTAGONIST-KILLER PROTEIN; BCL-2-ASSOCIATED X PROTEIN; BLOTTING, WESTERN; CASPASE 8; CASPASE 9; CELL PROLIFERATION; COLLAGEN TYPE IV; CYTOCHROMES C; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENCE; HEP G2 CELLS; HUMANS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA; PEPTIDES; RECOMBINANT PROTEINS; STAINING AND LABELING; TUMOR SUPPRESSOR PROTEIN P53;

EID: 70350761409     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm_00000276     Document Type: Article

References (21)

1. Saus J, Wieslander J, Langeveld JP, Quinones S and Hudson BG: Identification of the Goodpasture antigen as the alpha 3 (IV) chain of collagen IV. J Biol Chem 263: 13374-13380, 1988.
2. Maeshima Y, Colorado PC, Torre A, et al: Distinct antitumor properties of a type IV collagen domain derived from basement membrane. J Biol Chem 275: 21340-21348, 2000.
3. Colorado PC, Torre A, Kamphaus G, et al: Anti-angiogenic cues from vascular basement membrane collagen. Cancer Res 60: 2520-2526, 2000.
4. Kamphaus GD, Colorado PC, Panka DJ, et al: Canstatin, a novel matrix-derived inhibitor of angiogenesis and tumor growth. J Biol Chem 275: 1209-1215, 2000.
5. Maeshima Y, Colorado PC and Kalluri R: Two RGD-independent alpha vbeta 3 integrin binding sites on tumstatin regulate distinct anti-tumor properties. J Biol Chem 275: 23745-23750, 2000.
6. Petitclerc E, Boutaud A, Prestayko A, et al: New functions for non-collagenous domains of human collagen type IV. Novel integrin ligands inhibiting angiogenesis and tumor growth in vivo. J Biol Chem 275: 8051-8061, 2000.
7. Maeshima Y, Manfredi M, Reimer C, et al: Identification of the anti-angiogenic site within vascular basement membrane-derived tumstatin. J Biol Chem 276: 15240-15248, 2001.
8. Maeshima Y, Yerramalla UL, Dhanabal M, et al: Extracellular matrix-derived peptide binds to alpha(v)beta(3) integrin and inhibits angiogenesis. J Biol Chem 276: 31959-31968, 2001.
9. Maeshima Y, Sudhakar A, Lively JC, et al: Tumstatin, an endothelial cell-specific inhibitor of protein synthesis. Science 295: 140-143, 2002.
10. Floquet N, Pasco S, Ramont L, et al: The antitumor properties of the alpha3(IV)-(185-203) peptide from the NC1 domain of type IV collagen (tumstatin) are conformation-dependent. J Biol Chem 279: 2091-2100, 2004.
11. Wang SJ, Liu XH, JI YB and Chen N: Study on biological activity of two modified anti-tumor peptide of tumstatin. Prog Biochem Biophys 34: 1152-1161, 2007.
12. Desai BN, Myers BR and Schreiber SL: FKBP12-rapamycin-associated protein associates with mitochondria and senses osmotic stress via mitochondrial dysfunction. Proc Natl Acad Sci USA 99: 4319-4324, 2002.
13. Khor TO, Gul YA, Ithnin H and Seow HF: A comparative study of the expression of Wnt-1, WISP-1, survivin and cyclin-D1 in colorectal carcinoma. Int J Colorectal Dis 21: 291-300, 2006.
14. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES and Wang X: Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91: 479-489, 1997.
15. Brenner C and Kroemer G: Apoptosis. Mitochondria - the death signal integrators. Science 289: 1150-1151, 2000.
16. Green DR and Reed JC: Mitochondria and apoptosis. Science 281: 1309-1312, 1998.
17. Tsujimoto Y and Shimizu S: Bcl-2 family: life-or-death switch. FEBS Lett 466: 6-10, 2000.
18. Zhang L, Yu J, Park BH, Kinzler KW and Vogelstein B: Role of BAX in the apoptotic response to anticancer agents. Science 290: 989-992, 2000.
19. Gross A, Jockel J, Wei MC and Korsmeyer SJ: Enforced dimerization of BAX results in its translocation, mitochondrial dysfunction and apoptosis. EMBO J 17: 3878-3885, 1998.
20. Sheikh MS and Fornace AJ Jr: Role of p53 family members in apoptosis. J Cell Physiol 182: 171-181, 2000.
21. Bennett M, Macdonald K, Chan SW, Luzio JP, Simari R and Weissberg P: Cell surface trafficking of Fas: a rapid mechanism of p53-mediated apoptosis. Science 282: 290-293, 1998.