Site-directed mutagenesis of the cytochrome b gene and development of diagnostic methods for identifying QoI resistance of rice blast fungus

Pest Management Science

Volumn 65, Issue 12, 2009, Pages 1344-1351

  Wei, Chuan Zhao ^a   Katoh, Hiroshi ^a   Nishimura, Kumiko ^a   Ishii, Hideo ^a  

^a NATIONAL INSTITUTE FOR AGRO ENVIRONMENTAL SCIENCES   (Japan)

Author keywords

Allele specific PCR; Fungicide resistance; Magnaporthe oryzae; Molecular diagnosis; PCR Luminex; QoIs; Strobilurin fungicides

Indexed keywords

CYTOCHROME B; FUNGAL PROTEIN; FUNGICIDE;

ALLELE; CYTOCHROME; DISEASE RESISTANCE; FUNGAL DISEASE; FUNGICIDE; GENE EXPRESSION; MOLECULAR ANALYSIS; MUTAGENICITY; RICE;

ANTIFUNGAL RESISTANCE; ARTICLE; DRUG EFFECT; GENETICS; ISOLATION AND PURIFICATION; MAGNAPORTHE; METABOLISM; MICROBIOLOGY; MUTATION; PLANT DISEASE; RICE; SITE DIRECTED MUTAGENESIS;

CYTOCHROMES B; DRUG RESISTANCE, FUNGAL; FUNGAL PROTEINS; FUNGICIDES, INDUSTRIAL; MAGNAPORTHE; MUTAGENESIS, SITE-DIRECTED; MUTATION; ORYZA SATIVA; PLANT DISEASES;

BACTERIA (MICROORGANISMS); FUNGI; MAGNAPORTHE; MAGNAPORTHE GRISEA; MAGNAPORTHE ORYZAE;

EID: 70350721724     PISSN: 1526498X     EISSN: 15264998     Source Type: Journal    
DOI: 10.1002/ps.1821     Document Type: Article

References (27)

1. Bartlett DW, Clough JM, Godwin JR, Hall AA, Hamer M and Parr-Dobrzanski B,The strobilurin fungicides. Pest Manag Sci 58:649-662 (2002).
2. Gisi U, Chin KM, Knapova G, Küng-Färber R, Mohr U, Parisi S, et al, Recent developments in elucidating modes of resistance to phenylamide, DMI and strobilurin fungicides. Crop Prot 19:863-872 (2000).
3. Gisi U, Sierotzki H, Cook A and McCaffery A, Mechanisms influencing the evolution of resistance to Qo inhibitor fungicides. Pest Manag Sci 58:859-867 (2002).
4. Kuck KH, QoI fungicides: resistance mechanisms and its practical importance, in Pesticide Chemistry - Crop Protection, Public Health, Environmental Safety, ed. by Ohkawa H, Miyagawa H and Lee PW. Wiley VCH, Weinheim, Germany, pp. 275-283 (2007).
5. Vincelli P and Dixon E, Resistance to QoI (strobilurin-like) fungicides in isolates of Pyricularia grisea from perennial ryegrass. Plant Dis 86:235-240 (2002).
6. Kim YS, Dixon EW, Vincelli P and Farman ML, Field resistance to strobilurin (QoI) fungicides in Pyricularia grisea caused by mutations in the mitochondrial cytochrome b gene. Phytopathology 93:891-900 (2003).
7. Groth DE, Azoxystrobin rate and timing effects on rice head blast incidence and rice grain and milling yields. Plant Dis 90:1055-1058 (2006).
8. Stammler G, Itoh M, Hino I, Watanabe A, Kojima K, Motoyoshi M, et al, Efficacy of orysastrobin against blast and sheath blight in transplanted rice. J Pestic Sci 32:10-15 (2007).
9. Araki Y, Sugihara M, Sawada H, Fujimoto H and Masuko M, Monitoring of the sensitivity of Magnaporthe grisea to metominostrobin 2001-2003: no emergence of resistant strains and no mutations at codon 143 or 129 ofthe cytochromeb gene. J Pestic Sci 30:203-208 (2005).
10. Ishii H, Fraaije BA, Sugiyama T, Noguchi K, Nishimura K, Takeda T, et al, Occurrence and molecular characterization of strobilurin resistance in cucumber powdery mildew and downy mildew. Phytopathology 91:1166-1171 (2001).
11. Ishii H, Yano K, Date H, Furuta A, Sagehashi Y, Yamaguchi T, et al, Molecular characterization and diagnosis of QoI resistance in cucumber and eggplant fungal pathogens. Phytopathology 97:1458-1466 (2007).
12. Zheng D, Olaya G and Köller W, Characterization of laboratory mutants of Venturia inaequalis resistant to the strobilurin-related fungicide kresoxim-methyl. Curr Genet 38:148-155 (2000).
13. Fisher N, Brown AC, Sexton G, Cook A, Windass J and Meunier B, Modeling the Qo site of crop pathogens in Saccharomyces cerevisiae cytochrome b. Eur J Biochem 271:2264-2271 (2004).
14. Manandhar HK, Jørgensen HJL, Smedegaard-Petersen V and Mathur SB, Seed-borne infection of rice by Pyriculariaoryzae and its transmission to seedlings. Plant Dis 82:1093-1099 (1998).
15. Ishii H, Joseph-Horne T, Hollomon DW and Nishimura K, Synergism between strobilurin fungicides and cyanide-resistant respiration inhibitor n-propyl gallate (abstract in Japanese). Abstr Annu Meet Pestic Sci Soc Jpn 24:61 (1999).
16. Hollomon DW, Wood PM, Reeve C and Miguez M, Alternative oxidase and its impact on the activity of Qo and Qi site inhibitors, in Modern Fungicides and Antifungal Compounds IV, ed. by Dehne HW, Gisi U, Kuck KH, Russell PE and Lyr H. BCPC, Alton, UK, pp. 31-34 (2005).
17. Kaku K, Takagaki M, Shimizu T and Nagayama K, Diagnosis of dehydratase inhibitors in malanin biosynthesis inhibitor (MBI-D) resistance by primer-introduced restriction enzyme analysis in scytalone dehydratase gene of Magnaporthe grisea. Pest Manag Sci 59:843-846 (2003).
18. Avila-Adame CandKöller W, Characterization of spontaneousmutants of Magnaporthe grisea expressing stable resistance to the Qo-inhibiting fungicide azoxystrobin. Curr Genet 42:332-338 (2003).
19. Baumler S, Sierotzki H, Gisi U and Mohler V, Evaluation of Erysiphe graminis f. sp. tritici field isolates for resistance to strobilurin fungicides with different SNP detection systems. Pest Manag Sci 59:310-314 (2003).
20. Fraaije BA, Cools HJ, Fountaine J, Lovell DJ, Motteram J, West JS, et al, Role of ascospores in further spread of QoI-resistant cytochrome b alleles (G143A) in field population of Mycosphaerella graminicola. Phytopathology 95:933-941 (2005).
21. Ishii H, Tanoue J, Oshima M, Chung WH, Nishimura K, Yamaguchi J, et al, First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens. J Gen Plant Pathol 74:409-416 (2008).
22. Birren B, Green ED, Klapholz S, Myers RM and Roskams J, Analyzing DNA: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1997).
23. White TJ, Bruns T, Lee S and Taylor J, Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, in PCR Protocols: A Guide to Method and Applications, ed. by Innis MA, Gelfand DH, Sninsky JJ and White TJ. Academic Press, San Diego, CA, pp. 315-322 (1990).
24. Dojka MA, Hugenholtz P, Haack SK and Pace NR, Microbial diversity in a hydrocarbon- and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation. Appl Environ Microbiol 64:3869-3877 (1998).
25. Lane DJ, 16S/23S rRNA sequencing, in Nucleic Acid Techniques in Bacterial Systematics, ed. by Stackebrandt E and Goodfellow M. Wiley, New York, NY, pp. 115-175 (1991).
26. Grasso V, Palermo S, Sierotzki H, Garibaldi A and Gisi U, Cytochrome b gene structure and consequences for resistance to Qo inhibitor fungicides in plant pathogens. Pest Manag Sci 62:465-472 (2006).
27. Katoh H, Wei CZ and Ishii H, An attempt at simultaneous detection of strobilurin-resistant and MBI-D-resistant isolates of Magnaporthe grisea using PCR-Luminex system (abstract in Japanese). Jpn J Phytopathol 74:271 (2008).