Study on the debridement efficacy of formulated enzymatic wound debriding agents by in vitro assessment using artificial wound eschar and by an in vivo pig model: Technical Article

Wound Repair and Regeneration

Volumn 17, Issue 6, 2009, Pages 853-862

  Shi, Lei ^a   Ermis, Ryan ^a   Lam, Kan ^b   Cowart, Jerry ^c   Attar, Paul ^b   Aust, Duncan ^a  

^a Healthpoint Ltd   (United States)

^b Bridge PTS   (United States)

^c AIR FORCE RESEARCH LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTIFICIAL WOUND ESCHAR SUBSTRATE; COLLAGEN; ELASTIN; FIBRIN; FIBRINOGEN; PAPAIN; UNCLASSIFIED DRUG; WOUND HEALING PROMOTING AGENT;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BURN; CONCENTRATION RESPONSE; CONTROLLED STUDY; DEBRIDEMENT; DOSE RESPONSE; DRUG DOSE COMPARISON; DRUG EFFICACY; DRUG SYNTHESIS; ENZYME ACTIVITY; EPITHELIZATION; EXUDATE; FEMALE; GRANULATION TISSUE; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; SKIN BIOPSY; SKIN SURFACE; SWINE; WOUND HEALING;

ADMINISTRATION, TOPICAL; ANIMALS; BURNS; DEBRIDEMENT; DISEASE MODELS, ANIMAL; EMULSIONS; FEMALE; PAPAIN; SUS SCROFA; TREATMENT OUTCOME; WOUND HEALING;

EID: 70350648395     PISSN: 10671927     EISSN: 1524475X     Source Type: Journal    
DOI: 10.1111/j.1524-475X.2009.00545.x     Document Type: Article

References (22)

1. Clark RA. Cutaneous tissue repair: basic biologic considerations. I. J Am Acad Dermatol 1985 13 : 701 725.
2. Hellgren L, Vincent J. Debridement: an essential step in wound healing. In : Westerhoff W, editor. Leg ulcers: diagnosis and treatment. Amsterdam, the Netherlands : Elsevier Science, 1993 : 305 312.
3. Fowler E, van Rijswijk L. Using wound debridement to help achieve the goals of care. Ostomy Wound Manage 1995 41 : 23S 36S.
4. Berger MM. Enzyme debriding preparations. Ostomy Wound Manage 1993 39 : 61 2, 66-9.
5. Fowler E. Instrument/sharp debridement of non-viable tissue in wounds. Ostomy Wound Manage 1992 38 : 26, 28-30, 32-3.
6. Steed DL, Donohoe D, Webster MW, Lindsley L. Effect of extensive debridement and treatment on the healing of diabetic foot ulcers. J Am Coll Surg 1996 183 : 61 4.
7. Falanga V. Wound bed preparation and the role of enzymes: a case for multiple actions of therapeutic agents. Wounds 2002 14 : 47 57.
8. ® papain-urea debriding ointment: a historical review. Wounds 2003 15 (Suppl. 4) : 2S 12S.
9. Hebda PA, Flynn KJ, Dohar JE. Evaluation of the efficacy of enzymatic debriding agents for removal of necrotic tissue and promotion of healing in porcine skin wounds. Wounds 1998 10 : 83 96.
10. Skrabut EM, Hebda PA, Samuels JA, Richards SM, Edmunds T, Cunneen MF, Vaccaro CA, McPherson JM. Removal of necrotic tissue with an ananain-based enzyme-debriding preparation. Wound Repair Regen 1996 4 : 433 443.
11. Orgill DP, Liu PY, Ritterbush LS, Skrabut EM, Samuels JA, Shames SL. Debridement of porcine burns with a highly purified, ananain-based cysteine protease preparation. J Burn Care Rehabil 1996 17 : 311 322.
12. Mekkes JR, Le Poole IC, Das PK, Bos JD, Westerhof W. Efficient debridement of necrotic wounds using proteolytic enzymes derived from Antarctic krill: a double-blind, placebo-controlled study in a standardized animal wound model. Wound Repair Regen 1998 6 : 50 7.
13. Mekkes JR, Zeegelaar JE, Westerhof W. Quantitative and objective evaluation of wound debriding properties of collagenase and fibrinolysin/ deoxyribonuclease in a necrotic ulcer animal model. Arch Dermatol Res 1998 290 : 152 157.
14. Sibbald RG, Williamson D, Orsted HL, Campbell K, Keast D, Krasner D, Sibbald D. Preparing the wound bed: debridement, bacterial balance, and moisture balance. Ostomy Wound Manage 2000 46 : 14 22, 24-8, 30-5.
15. Alvarez OM, Fernandez-Obregon A, Rogers RS, Bergamo L, Masso J, Black M. A prospective, randomized, comparative study of collagenase and papain-urea for pressure ulcer debridement. Wounds 2002 14 : 293 301.
16. Thomas AML, Harding KG, Moore K. The structure and composition of chronic wound eschar. J Wound Care 1999 8 : 285 287.
17. Durham DR, Fortney DZ, Nanney LB. Preliminary evaluation of vibriolysin, a novel proteolytic enzyme composition suitable for the debridement of burn wound eschar. J Burn Care Rehabil 1993 14 : 544 551.
18. Hebda PA, Lo CY. The effects of active ingredients of standard debriding agent - papain and collagenase - on digestion of native and denatured collagenous substrates, fibrin and elastin. Wounds 2001 13 : 190 194.
19. Baici A, Cohen G, Fehr K, Böni A. A handy assay for collagenase using reconstituted fluorescein-labeled collagen fibrils. Anal Biochem 1980 108 : 230 232.
20. Rinderknecht H, Geokas MC, Silverman P, Lillard Y, Haverback BJ. New methods for the determination of elastase. Clin Chim Acta 1968 19 : 327 329.
21. Silverstein P, Ruzicka FJ, Helmkamp GM Jr., Lincoln RA Jr., Mason AD Jr. In vitro evaluation of enzymatic debridement of burn wound eschar. Surgery 1973 73 : 15 22.
22. Hobson D, White E, Anderson L, Lira L. Development and use of a quantitative method to evaluate the action of enzymatic wound debriding agents in vitro. Wounds 1998 10 : 105 110.