Removal of necrotic tissue with an ananain-based enzyme-debriding preparation

Wound Repair and Regeneration

Volumn 4, Issue 4, 1996, Pages 433-443

  Skrabut, Eugene M ^a   Hebda, Patricia A ^b   Samuels, Jennifer A ^a   Richards, Susan M ^a   Edmunds, Tim ^a   Cunneen, Michele F ^a   Vaccaro, Charles A ^a   McPherson, John M ^a  

^a Genzyme Tissue Repair   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0347252739     PISSN: 10671927     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1524-475X.1996.40407.x     Document Type: Article

References (29)

1. Rowan AD, Buttle DJ, Barrett AJ. Ananain: a novel cysteine protease found in pineapple stem. Arch Biochem Biophys 1988;267:262-70.
2. Rowan AD, Buttle DJ, Barrett AJ. The cysteine proteinases of the pineapple plant. Biochem J 1990;266:869-75.
3. Napper AD, Bennett SP, Borowski M, Holdridge MB, Leonard MJC, Rogers EE, Duan Y, Laursen RA, Reinhold B, Shames SL. Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain. Biochem J 1994;301:727-35.
4. Bergstrom N, Bennett MA, Carlson CE, et al. Treatment of pressure ulcers. Clinical practice guideline, No. 15. Rockville, MD: U.S. Department of Health and Human Services. Public Health Service, Agency for Health Care Policy and Research. AHCPR Publication No. 95-0652, 1994.
5. Cooreman WM, Scharpe S, Demeester J, Lauwers A. Bromelain, biochemical and pharmacological properties. Pharm Acta Helv 1976;51:73-96.
6. Taussig SJ, Batkin S. Bromelain, the enzyme complex of pineapple (Ananas comosus) and its clinical application. An update. J Ethnopharm 1988; 22:191-203.
7. Levine N, Seifter E, Connerton C, Levenson SM. Debridement of experimental skin burns of pigs with bromelain, a pineapplestem enzyme. Plast Reconstr Surg 1973;52:413-24.
8. Houck JC, Chang CM, Klein G. Isolation of an effective debriding agent from the stems of pineapple plants. Int J Tissue React 1983;5:125-34.
9. Ahle NW, Hamlet MP. Enzymatic frostbite eschar debridement by bromelain. Ann Emerg Med 1987;16:1063-5.
10. Rowan AD, Christopher C, Kelly SF, Buttle DJ, Ehrlich HP. Debridement of experimental full-thickness skin burns of rats with enzyme fractions derived from pineapple stem. Burns 1990;16:243-6.
11. Dimick AR. Experience with the use of proteolytic enzyme (Travase) in burn patients. J Trauma 1977;76:48-55.
12. Garret TA. Bacillus subtilis protease: a new topical agent for debridement. Clin Med 1969;76:11-5.
13. Hummel RP, Kautz PD. The continuing problem of sepsis following enzymatic debridement of burns. J Trauma 1974;14: 572-9.
14. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227;680-5.
15. Delmar EG, Largman C, Brodrick JW, Geokas MC. A sensitive new substrate for chymotrypsin. Anal Biochem 1979;99:316-20.
16. Hebda PA. The acceleration of epidermal wound healing in partial thickness burns by transforming growth factor-beta. J Invest Dermatol 1989;92:442.
17. Hebda PA, Delaney GS, Skrabut EM. Debridement of partial thickness burns in porcine skin by ananain and comosain, two plant derived proteases. J Invest Derm 1991;96:580.
18. Davis SC, Mertz PM, Eaglstein WH. Second-degree burn healing: the effect of occlusive dressings and a cream. J Surg Res 1990;48:245-8.
19. Eriksson E, Miles RD, Le HQ. Changes in blood flow and me-tabolism in a neurovascular island flap after a burn. Ann Plast Surg 1986;17:79-81.
20. Orgill DP, Liu PY, Ritterbush LS, Skrabut EM, Samuels JA, Shames SL. Debridement of porcine burns with a highly purified, ananain-based cysteine protease preparation. J Burn Care Rehab 1996; 17:311-22.
21. Mertz PM, Hebda PA, Eaglstein WH. A porcine model for evaluating epidermal wound healing. In: Tumbleson ME, editor. Swine in biomedical research, vol. 1. New York: Plenum Publishing, 1986:291-302.
22. Dixon WJ, Brown MB, Engelman L, Jennrich RI, editors. BMDP Statistical Software. Berkeley: University of California Press, 1990.
23. Steele RGD, Torrie JH. Principles and procedures of statistics, 2nd ed. New York: McGraw-Hill, 1980:504-6.
24. Monte KA, Constantine BE, Shannon RJ, Bolton LL. A wound model for ischemic ulcers in the guinea pig. J Invest Derm 1992;98:585.
25. Wallace DG, McPherson JM, Ellingsworth LE, Cooperman L, Armstrong R, Piez KA. Injectable collagen for tissue augmentation. In: Nimni M, editor. Collagen: biochemistry, biomechanics, and biotechnology. Vol 3. Boca Raton: CRC Press, 1988:117-44.
26. Clark RAF. Overview and general considerations of wound repair. In: Clark RAF, Henson PM, editors. The molecular and cellular biology of wound repair. New York: Plenum Press, 1988:3-33.
27. Silverstein P, Maxwell P, Duckett, L. Enzymatic Debridement. In: Boswick JA, editor. The art and science of burn care. Rockville: Aspen Publishers, 1987:75-81.
28. Brown D, Garner W, Young VL. Skin grafting: dermal components in inhibition of wound contraction. South Med J 1990;83:789-95.
29. Wainwright DJ. Use of an acellular allograft dermal matrix (AlloDerm) in the management of full-thickness burns. Burns 1995;21:243-8.