Cytotoxicity of quahog parasite unknown (QPX) toward hard clam (Mercenaria mercenaria) haemocytes and interactions between different pathogen isolates and host strains

Parasitology

Volumn 136, Issue 11, 2009, Pages 1281-1289

  Perrigault, Mickael ^a   Allam, Bassem ^a  

^a STONY BROOK UNIVERSITY   (United States)

Author keywords

Cytotoxicity; Extracellular products; Haemocyte; Hard clam; Mercenaria mercenaria; Neutral red uptake; QPX; Thraustochytrid; Virulence factors

Indexed keywords

VIRULENCE FACTOR;

ARTICLE; BLOOD CELL; CELL PROLIFERATION; CLAM; CYTOTOXICITY; HOST PATHOGEN INTERACTION; HOST RESISTANCE; IN VITRO STUDY; LABORATORY; MERCENARIA; MERCENARIA MERCENARIA; NONHUMAN; PARASITE; PATHOGENESIS; PRIORITY JOURNAL; QUAHOG PARASITE UNKNOWN; VIRULENCE;

ANIMALS; HEMOCYTES; HOST-PARASITE INTERACTIONS; MERCENARIA; NEUTRAL RED; PARASITES; VIRULENCE FACTORS;

BIVALVIA; MERCENARIA MERCENARIA; THRAUSTOCHYTRIIDAE;

EID: 70350570418     PISSN: 00311820     EISSN: 14698161     Source Type: Journal    
DOI: 10.1017/S0031182009990606     Document Type: Article

References (41)

1. Allam, B., Paillard, C. and Ford, S. E. (2002). Pathogenicity of Vibrio tapetis, the etiological agent of brown ring disease in clams. Diseases of Aquatic Organisms 48, 221-231.
2. Anderson, R. S., Kraus, B. S., McGladdery, S. and Smolowitz, R. (2003). QPX, a pathogen of quahogs (hard clams), employs mucoid secretions to resist host antimicrobial agents. Journal of Shellfish Research 22, 205-208.
3. Anderson, R. S., Luskey, T. M. and Strauss, M. A. (2006). In vitro protease production by QPX (Abstract). Journal of Shellfish Research 25, 708.
4. Borenfreund, E. and Puerner, J. A. (1985a). A simple quantitative procedure using monolayer cultures for cytoxicity assays (HTD/NR-90). Journal of Tissue Culture Methods 9, 7-9.
5. Borenfreund, E. and Puerner, J. A. (1985b). Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicology Letters 24, 119-124.
6. Bower, S. M. (1987). Pathogenicity and host specificity of Labyrinthuloides haliotidis (Protozoa, Labyrinthomorpha), a parasite of juvenile abalone. Canadian Journal of Zoology 65, 2008-2012.
7. Buggé , D.M. and Allam, B. (2005). A fluorometric technique for the in vitro measurement of growth and viability in Quahog parasite unknown (QPX). Journal of Shellfish Research 24, 1013-1018.
8. Bushek, D. and Allen, S. K. (1996a). Host-parasite interactions among broadly distributed populations of the eastern oyster Crassostrea virginica and the protozoan Perkinsus marinus. Marine Ecology-Progress Series 139, 127-141.
9. Bushek, D. and Allen, S. K. (1996b). Races of Perkinsus marinus. Journal of Shellfish Research 15, 103-107.
10. Cajaraville, M. P., Olabarrieta, I. and Marigomez, I. (1996). In vitro activities in mussel hemocytes as biomarkers of environmental quality: A case study in the Abra Estuary (Biscay Bay). Ecotoxicology and Environmental Safety 35, 253-260.
11. Calvo, L. M. R., Ford, S. E., Kraeuter, J. N., Leavitt, D. F., Smolowitz, R. and Burreson, E. M. (2007). Influence of host genetic origin and geographic location on QPX disease in Northern quahogs (=hard clams), Mercenaria mercenaria. Journal of Shellfish Research 26, 109-119. (Pubitemid 46724635)
12. Calvo, L. M. R., Walker, J. G. and Burreson, E. M. (1998). Prevalence and distribution of QPX, Quahog Parasite Unknown, in hard clams Mercenaria mercenaria in Virginia, USA. Diseases of Aquatic Organisms 33, 209-219.
13. Choquet, G., Soudant, P., Lambert, C., Nicolas, J. L. and Paillard, C. (2003). Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis. Diseases of Aquatic Organisms 57, 109-116.
14. Chu, F. L. E., Volety, A. K., Hale, R. C. and Huang, Y. Q. (2002). Cellular responses and disease expression in oysters (Crassostrea virginica) exposed to suspended field - contaminated sediments. Marine Environmental Research 53, 17-35.
15. Coles, J. A., Farley, S. R. and Pipe, R. K. (1995). Alteration of the immune response of the common marine mussel Mytilus edulis resulting from exposure to cadmium. Diseases of Aquatic Organisms 22, 59-65.
16. Dahl, S., Perrigault, M. and Allam, B. (2008). Laboratory transmission studies of QPX disease in the hard clam: Interactions between different host strains and pathogen isolates. Aquaculture 280, 64-70.
17. Dove, A. D. M., Bowser, P. R. and Cerrato, R. M. (2004). Histological analysis of an outbreak of QPX disease in wild hard clams Mercenaria mercenaria in New York. Journal of Aquatic Animal Health 16, 246-250.
18. Earnhart, C. G., Vogelbein, M. A., Brown, G. D., Reece, K. S. and Kaattari, S. L. (2004). Supplementation of Perkinsus marinus cultures with host plasma or tissue homogenate enhances their infectivity. Applied and Environmental Microbiology 70, 421-431.
19. Ford, S. E. (2001). Pests, parasites, diseases and defense mechanisms of the hard clam, Mercenaria mercenaria. In Biology of the Hard Clam, (ed. Kraeuter, J. N. and Castagna, M.), pp. 591-628. Elsevier Science, Amsterdam.
20. The Netherlands. Ford, S. E., Kraeuter, J. N., Barber, R. D. and Mathis, G. (2002). Aquaculture-associated factors in QPX disease of hard clams: density and seed source. Aquaculture 208, 23-38.
21. Garreis, K. A., La Peyre, J. F. and Faisal, M. (1996). The effects of Perkinsus marinus extracellular products and purified proteases on oyster defence parameters in vitro. Fish and Shellfish Immunology 6, 581-597.
22. Grundy, M. M., Moore, M. N., Howell, S. M. and Ratclifie, N. A. (1996). Phagocytic reduction and effects on lysosomal membranes by polycyclic aromatic hydrocarbons, in haemocytes of Mytilus edulis. Aquatic Toxicology 34, 273-290.
23. Jain, R., Raghukumar, S., Tharanathan, R. and Bhosle, N. B. (2005). Extracellular polysaccharide production by thraustochytrid protists. Marine Biotechnology 7, 184-192.
24. Kleinschuster, S. J., Smolowitz, R. and Parent, J. (1998). In vitro life cycle and propagation of quahog parasite unknown. Journal of Shellfish Research 17, 75-78.
25. Lane, E. and Birkbeck, T. H. (2000). Species specificity of some bacterial pathogens of bivalve molluscs is correlated with their interaction with bivalve haemocytes. Journal of Fish Diseases 23, 275-279.
26. Le Sueur, L. D., Collares-Buzato, C. B., Kalapothakis, E. and Da Cruz-Hofing, M. A. (2005). In vitro Effect of the Phoneutria nigriventer spider venom on cell viability, paracellular barrier function and transcellular transport in cultured cell lines. Toxicon 46, 130-141.
27. Maas, P. A. Y., Kleinschuster, S. J., Dykstra, M. J., Smolowitz, R. and Parent, J. (1999). Molecular characterization of QPX (Quahog Parasite Unknown), a pathogen of Mercenaria mercenaria. Journal of Shellfish Research 18, 561-567.
28. MacIntyre, E. A., Earnhart, C. G. and Kaattari, S. L. (2003). Host oyster tissue extracts modulate in vitro protease expression and cellular differentiation in the protozoan parasite, Perkinsus marinus. Parasitology 126, 293-302.
29. Matozzo, V., Ballarin, L. and Marin, M. G. (2002). In vitro effects of tributyltin on functional responses of haemocytes in the clam Tapes philippinarum. Applied Organometallic Chemistry 16, 169-174.
30. Muehlstein, L. K., Porter, D. and Short, F. T. (1988). Labyrinthula sp., a marine slime-mold producing the symptoms of wasting disease in eelgrass, Zostera marina. Marine Biology 99, 465-472.
31. Perrigault, M., Buggé , D. M., Hao, C. C. and Allam, B. (2009). Modulatory effects of hard clam (Mercenaria mercenaria) tissue extracts on the in vitro growth of its pathogen QPX. Journal of Invertebrate Pathology 100, 1-8.
32. Pichardo, S., Jos, A., Zurita, J., Salguero, M., Camean, A. and Repetto, G. (2006). Toxic effects produced by microcystins from a natural cyanobacterial bloom and a Microcystis aeruginosa isolated strain on the fish cell lines RTG-2 and PLHC-1. Archives of Environmental Contamination and Toxicology 51, 86-96.
33. Pipe, R. K., Coles, J. A., Carissan, F. M. M. and Ramanathan, K. (1999). Copper induced immunomodulation in the marine mussel, Mytilus edulis. Aquatic Toxicology 46, 43-54.
34. Qian, H., Liu, Q. Q., Allam, B. and Collier, J. L. (2007). Molecular genetic variation within and among isolates of QPX (Thraustochytridae), a parasite of the hard clam Mercenaria mercenaria. Diseases of Aquatic Organisms 77, 159-168.
35. Ragan, M. A., MacCallum, G. S., Murphy, C. A., Cannone, J. J., Gutell, R. R. and McGladdery, S. E. (2000). Protistan parasite QPX of hard shell clam Mercenaria mercenaria is a member of Labyrinthulomycota. Diseases of Aquatic Organisms 42, 185-190.
36. Raghukumar, S. (2002). Ecology of the marine protists, the Labyrinthulomycetes (Thraustochytrids and Labyrinthulids). European Journal of Protistology 38, 127-145.
37. Reece, K. S., Bushek, D., Hudson, K. L. and Graves, J. E. (2001). Geographic distribution of Perkinsus marinus genetic strains along the Atlantic and Gulf coasts of the USA. Marine Biology 139, 1047-1055.
38. Smolowitz, R., Leavitt, D. and Perkins, F. (1998). Observations of a protistan disease similar to QPX in Mercenaria mercenaria (hard clams) from the coast of Massachusetts. Journal of Invertebrate Pathology 71, 9-25.
39. Stokes, N. A., Calvo, L. M. R., Reece, K. S. and Burreson, E. M. (2002). Molecular diagnostics, field validation, and phylogenetic analysis of Quahog Parasite Unknown (QPX), a pathogen of the hard clam Mercenaria mercenaria. Diseases of Aquatic Organisms 52, 233-247.
40. Whyte, S. K., Cawthorn, R. J. and McGladdery, S. E. (1994). QPX (Quahaug Parasite X), a pathogen of northern quahaug Mercenaria mercenaria from the gulf of St Lawrence, Canada. Diseases of Aquatic Organisms 19, 129-136.
41. Zaroogian, G., Anderson, S. and Voyer, R. A. (1992). Individual and combined cytotoxic effects of cadmium, copper, and nickel on brown cells of Mercenaria mercenaria. Ecotoxicology and Environmental Safety 24, 328-337.