A fluorometric technique for the in vitro measurement of growth and viability in quahog parasite unknown (QPX)

Journal of Shellfish Research

Volumn 24, Issue 4, 2005, Pages 1013-1018

  Buggé, Deenie M ^a   Allam, Bassem ^a  

^a STONY BROOK UNIVERSITY   (United States)

Author keywords

Clam; Fluorescein diacetate; In vitro; Mercenaria mercenaria; Northern quahog

Indexed keywords

BIVALVE; FLUOROMETRY; HOST-PARASITE INTERACTION; MEASUREMENT METHOD; PARASITE; PATHOGEN;

BIVALVIA; MERCENARIA MERCENARIA; PROTISTA;

EID: 33746913081     PISSN: 07308000     EISSN: None     Source Type: Journal    
DOI: 10.2983/0730-8000(2005)24[1013:AFTFTI]2.0.CO;2     Document Type: Article

References (29)

1. Anderson, R. S. & A. E. Beaven. 2001. A comparative study of anti-Perkinus marinus activity in bivalve sera. J. Shellfish Res. 20(3):1011-1017.
2. Anderson, R. S., B. S. Kraus, S. McGladdery & R. Smolowitz. 2003. QPX, A pathogen of quahogs (hard clams), employs mucoid secretions to resist host antimicrobial agents. J. Shellfish Res. 22(1):205-208.
3. Argüello-Garciá, R., M. Cruz-Soto, L. Romero-Montoya & L. Ortega-Pierres. 2004. Variability and variation in drug susceptibility among Giardia duodenalis isolates and clones exposed to 5-nitroimidazoles and benzimidazoles in vitro. J. Antimicrob. Chemother. 54:711-721.
4. Bernhard, J. M., S. G. Newkirk & S. Bowser. 1995. Towards a non-terminal viability assay for foraminiferan protists. J. Eukaryot. Microbiol. 42(4):357-367.
5. Bogaerts, P., J. Bohatier & F. Bonnemoy. 2001. Use of the ciliated protozoan Tetrahymena pyriformis for the assessment of toxicity and quantitative structure activity relationships of xenobiotics: Comparison with the microtox test. Ecotoxicol. Environ. Saf. 49:293-301.
6. Brothers, C., E. Marks, III & R. Smolowitz. 2000. Conditions affecting the growth and zoosporulation of the protistan parasite QPX in culture. Biol. Bull. 199:200-201.
7. Dayeh, V. A., S. L. Chow, K. Schirmer, D. H. Lynn & N. C. Bols. 2004. Evaluating the toxicity of Triton X-100 to protozoan, fish, and mammalian cells using fluorescent dyes as indicators of cell viability. Ecotoxicol Environ. Saf. 57:375-382.
8. Dove, A. D. M., P. R. Bowser & R. M. Cerrato. 2004. Histological analysis of an outbreak of QPX disease in wild hard clams Mercenaria mercenaria in New York. J. Aquat. Anim. Health 16:246-250.
9. Drinnan, R. E. & E. B. Henderson. 1963. 1962 mortalities and a possible disease organism in Neguac quahaugs. Biological Station at St. Andrews, Annual Report B11, New Brunswick.
10. Dungan, C. F. & R. M. Hamilton. 1995. Use of a tetrazolium-based cell proliferation assay to measure effects of in vitro conditions on Perkinsus marinus (Apicomplexa) proliferation. J. Eukaryot. Microbiol. 42(4):379-388.
11. Elandalloussi, L. M., R. Afonso, P. A. Nunes & M. L. Cancela. 2003. Effect of desferrioxamine and 2,2′-bipyridyl on the proliferation of Perkinsus atlanticus. Biomol. Eng. 20(4-6):349-354.
12. Ford, S. E., J. N. Kraeuter, R. D. Barber & G. Mathis. 2002. Aquaculture-associated factors in QPX disease of hard clams: density and seed source. Aquaculture 208:23-38.
13. Gauthier, J. D. & G. R. Vasta. 1994. Inhibition of in vitro replication of the oyster parasite Perkinsus marinus by the natural iron chelators transferrin, lactoferrin, and desferrioxamine. Dev. Comp. Immunol. 18(4):277-286.
14. Gauthier, J. D. & G. R. Vasta. 1995. In vitro culture of the eastern oyster parasite Perkinsus marinus: Optimization of the methodology. J. Invertebr. Pathol. 66:156-168.
15. Gauthier, J. D., B. Feig & G. R. Vasta. 1995. Effect of fetal bovine serum glycoproteins on the in vitro proliferation of the oyster parasite Perkinsus marinus: development of a fully defined medium. J. Eukaryot. Microbiol. 42(3):307-313.
16. Gauthier, J. D. & G. R. Vasta. 2002. Effects of plasma from bivalve mollusk species on the in vitro proliferation of the protistan parasite Perkinus marinus. J. Exp. Zool. 292:221-230.
17. Gauthier, J. D. & G. R. Vasta. 1993. Continuous in vitro culture of the eastern oyster parasite Perkinsus marinus. J. Invertebr. Pathol. 62:321-323.
18. Kleinschuster, S. J., R. Smolowitz & J. Parent. 1998. In vitro life cycle and propagation of quahog parasite unknown. J. Shellfish Res. 17(1):75-78.
19. La Peyre, J. F., M. Faisal & E. M. Burreson. 1993. In vitro propagation of the protozoan Perkinsus marinus, a pathogen of the eastern oyster Crassostrea virginica. J. Eukaryot. Microbiol. 40(3):304-310.
20. La Peyre, J. F. 1996. Propagation and in vitro studies of Perkinsus marinus. J. Shellfish Res. 15(1):89-101.
21. La Peyre, J. F. & M. Faisal. 1997. Development of a protein-free chemically defined culture medium for the propogation of the oyster pathogen Perkinsus marinus. Parasite 4:67-73.
22. Murphy, A. M. & T. J. Cowles. 1997. Effects of darkness on multi-excitation in vivo fluorescence and survival in a marine diatom. Limnol. Oceanogr. 42(6):1444-1453.
23. O'Farrell, C. L., J. F. La Peyre, K. T. Paynter & E. M. Burreson. 2000. Osmotic tolerance and volume regulation in in vitro cultures of the oyster pathogen Perkinsus marinus. J. Shellfish Res. 19(1):139-145.
24. Ragone Calvo, L. M., J. G. Walker & E. M. Burreson. 1998. Prevalence and distribution of QPX, quahog parasite unknown, in hard clams Mercenaria mercenaria in Virginia, USA. Dis. Aquatic Org. 33:209-219.
25. Schupp, D. G. & S. L. Erlandsen. 1987. A new method to determine Giardia cyst viability: correlation of fluorescein diacetate and propidium iodide staining with animal infectivity. Appl. Environ. Microbiol. 53(4):704-707.
26. Smolowitz, R., D. Leavitt & F. Perkins. 1998. Observations of a protistan disease similar to QPX in Mercenaria mercenaria (hard clams) from the coast of Massachusetts. J. Invertebr. Pathol. 71:9-25.
27. Stokes, N. A., L. M. Ragone Calvo, K. S. Reece & R. E. Burreson. 2002. Molecular diagnostics, field validation, and phylogenetic analysis of Quahog Parasite Unknown (QPX), a pathogen of the hard clam Mercenaria mercenaria. Dis. Aquatic Org. 52:233-247.
28. Volety, A. K. & W. S. Fisher. 2000. In vitro killing of Perkinsus marinus by hemocytes of Crassostrea virginica. J. Shellfish Res. 19(2):827-834.
29. Whyte, S. K., R. J. Cawthorn & S. E. McGladdery. 1994. QPX (Quahog Parasite X), a pathogen of northern quahog Mercenaria mercenaria from the Gulf of St. Lawrence, Canada. Dis. Aquatic Org. 19:129-136.