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Volumn 52, Issue 3, 2002, Pages 233-247

Molecular diagnostics, field validation, and phylogenetic analysis of Quahog Parasite Unknown (QPX), a pathogen of the hard clam Mercenaria mercenaria

Author keywords

Disease; Hard clams; Molecular techniques; PCR; Probe; QPX

Indexed keywords

MOLECULAR ANALYSIS; MOLLUSC; PARASITIC DISEASE; PROTIST;

EID: 0037059215     PISSN: 01775103     EISSN: None     Source Type: Journal    
DOI: 10.3354/dao052233     Document Type: Article
Times cited : (59)

References (45)
  • 1
    • 84986513723 scopus 로고
    • Development of a diagnostic test for Mikrocytos roughleyi, the aetiological agent of Australian winter mortality of the commercial rock oyster, Saccostrea commercialis (Iredale & Roughley)
    • Adlard RD, Lester RJG (1995) Development of a diagnostic test for Mikrocytos roughleyi, the aetiological agent of Australian winter mortality of the commercial rock oyster, Saccostrea commercialis (Iredale & Roughley). J Fish Dis 18:609-614
    • (1995) J Fish Dis , vol.18 , pp. 609-614
    • Adlard, R.D.1    Lester, R.J.G.2
  • 3
    • 84986436981 scopus 로고
    • Molecular diagnosis of Marteilia sydneyi (Paramyxea) in Sydney rock oysters, Saccostrea commercialis (Angas)
    • Anderson TJ, Adlard RD, Lester RJG (1995) Molecular diagnosis of Marteilia sydneyi (Paramyxea) in Sydney rock oysters, Saccostrea commercialis (Angas). J Fish Dis 18:507-510
    • (1995) J Fish Dis , vol.18 , pp. 507-510
    • Anderson, T.J.1    Adlard, R.D.2    Lester, R.J.G.3
  • 4
    • 0034129096 scopus 로고    scopus 로고
    • Phylogenetic analysis of the small subunit ribosomal RNA of Marteilia refringens validates the existence of phylum Paramyxea (Desportes and Perkins, 1990)
    • Berthe FCJ, Le Roux F, Peyretaillade E, Peyret P, Rodriguez D, Gouy M, Vivarès CP (2000) Phylogenetic analysis of the small subunit ribosomal RNA of Marteilia refringens validates the existence of phylum Paramyxea (Desportes and Perkins, 1990). J Eukaryot Microbiol 47:288-293
    • (2000) J Eukaryot Microbiol , vol.47 , pp. 288-293
    • Berthe, F.C.J.1    Le Roux, F.2    Peyretaillade, E.3    Peyret, P.4    Rodriguez, D.5    Gouy, M.6    Vivarès, C.P.7
  • 5
    • 0001377249 scopus 로고
    • Labyrinthuloides haliotidis n.sp. (Protozoa: Labyrinthomorpha), a pathogenic parasite of small juvenile abalone in a British Columbia mariculture facility
    • Bower SM (1987) Labyrinthuloides haliotidis n.sp. (Protozoa: Labyrinthomorpha), a pathogenic parasite of small juvenile abalone in a British Columbia mariculture facility. Can J Zool 65:1996-2007
    • (1987) Can J Zool , vol.65 , pp. 1996-2007
    • Bower, S.M.1
  • 6
    • 0034726984 scopus 로고    scopus 로고
    • Development of a PCR assay for detection of the oyster pathogen Bonamia ostreae and support for its inclusion in the Haplosporidia
    • Carnegie RB, Barber BJ, Culloty SC, Figueras AJ, Distel DL (2000) Development of a PCR assay for detection of the oyster pathogen Bonamia ostreae and support for its inclusion in the Haplosporidia. Dis Aquat Org 42:199-206
    • (2000) Dis Aquat Org , vol.42 , pp. 199-206
    • Carnegie, R.B.1    Barber, B.J.2    Culloty, S.C.3    Figueras, A.J.4    Distel, D.L.5
  • 7
    • 0033845555 scopus 로고    scopus 로고
    • Detection of Bonamia ostreae based on small subunit ribosomal probe
    • Cochennec N, Le Roux F, Berthe F, Gerard A (2000) Detection of Bonamia ostreae based on small subunit ribosomal probe. J Invertebr Pathol 76:26-32.
    • (2000) J Invertebr Pathol , vol.76 , pp. 26-32
    • Cochennec, N.1    Le Roux, F.2    Berthe, F.3    Gerard, A.4
  • 8
    • 0033672383 scopus 로고    scopus 로고
    • Use of competitive PCR to detect and quantify Haplosporidium nelsoni infection (MSX disease) in the eastern oyster (Crassostrea virginica)
    • Day JM, Franklin DE, Brown BL (2000) Use of competitive PCR to detect and quantify Haplosporidium nelsoni infection (MSX disease) in the eastern oyster (Crassostrea virginica). Mar Biotechnol 2:456-465
    • (2000) Mar Biotechnol , vol.2 , pp. 456-465
    • Day, J.M.1    Franklin, D.E.2    Brown, B.L.3
  • 10
    • 0027364817 scopus 로고
    • Small subunit ribosomal RNA gene sequence of the parasitic protozoan Haplosporidium nelsoni provides a molecular probe for the oyster MSX disease
    • Fong D, Chan MMY, Rodriguez D, Chen CC, Liang Y, Littlewood DTJ, Ford SE (1993) Small subunit ribosomal RNA gene sequence of the parasitic protozoan Haplosporidium nelsoni provides a molecular probe for the oyster MSX disease. Mol Biochem Parasitol 62:139-143
    • (1993) Mol Biochem Parasitol , vol.62 , pp. 139-143
    • Fong, D.1    Chan, M.M.Y.2    Rodriguez, D.3    Chen, C.C.4    Liang, Y.5    Littlewood, D.T.J.6    Ford, S.E.7
  • 11
    • 0031417936 scopus 로고    scopus 로고
    • Evidence that QPX (Quahog Parasite Unknown) is not present in hatchery-produced hard clam seed
    • Ford SE, Smolowitz R, Ragone Calvo LM, Barber RD, Kraueter JN (1997) Evidence that QPX (Quahog Parasite Unknown) is not present in hatchery-produced hard clam seed. J Shellfish Res 16:519-521
    • (1997) J Shellfish Res , vol.16 , pp. 519-521
    • Ford, S.E.1    Smolowitz, R.2    Ragone Calvo, L.M.3    Barber, R.D.4    Kraueter, J.N.5
  • 12
    • 0034713370 scopus 로고    scopus 로고
    • Monitoring Piscirickettsia salmonis by denaturant gel electrophoresis and competitive PCR
    • Heath S, Pak S, Marshall S, Prager EM, Orrego C (2000) Monitoring Piscirickettsia salmonis by denaturant gel electrophoresis and competitive PCR. Dis Aquat Org 41:19-29
    • (2000) Dis Aquat Org , vol.41 , pp. 19-29
    • Heath, S.1    Pak, S.2    Marshall, S.3    Prager, E.M.4    Orrego, C.5
  • 13
    • 0032030211 scopus 로고    scopus 로고
    • Validation of polymerase chain reaction-based techniques for proxy detection of bacterial fish pathogens: Framework, problems, and possible solutions for environmental applications
    • Hiney MP, Smith PR (1998) Validation of polymerase chain reaction-based techniques for proxy detection of bacterial fish pathogens: Framework, problems, and possible solutions for environmental applications. Aquaculture 162:41-68
    • (1998) Aquaculture , vol.162 , pp. 41-68
    • Hiney, M.P.1    Smith, P.R.2
  • 15
    • 0001257255 scopus 로고
    • Ultrastructural observations on a Thraustochytrid fungus parasitic in the gills of squid (Ilex illecebrosus Lesueur)
    • Jones GM, O'Dor RK (1983) Ultrastructural observations on a Thraustochytrid fungus parasitic in the gills of squid (Ilex illecebrosus Lesueur). J Parasitol 69:903-911
    • (1983) J Parasitol , vol.69 , pp. 903-911
    • Jones, G.M.1    O'Dor, R.K.2
  • 16
    • 0034646369 scopus 로고    scopus 로고
    • Molecular detection of Marteilia sydneyi, pathogen of Sydney rock oysters
    • Kleeman SN, Adlard RD (2000) Molecular detection of Marteilia sydneyi, pathogen of Sydney rock oysters. Dis Aquat Org 40:137-146
    • (2000) Dis Aquat Org , vol.40 , pp. 137-146
    • Kleeman, S.N.1    Adlard, R.D.2
  • 17
    • 0031876107 scopus 로고    scopus 로고
    • In vitro life cycle and propagation of Quahog Parasite Unknown
    • Kleinschuster SJ, Smolowitz R, Parent J (1998) In vitro life cycle and propagation of Quahog Parasite Unknown. J Shellfish Res 17(1):75-78
    • (1998) J Shellfish Res , vol.17 , Issue.1 , pp. 75-78
    • Kleinschuster, S.J.1    Smolowitz, R.2    Parent, J.3
  • 18
    • 0029360565 scopus 로고
    • Characterization of the small subunit ribosomal RNA gene of the oyster parasite Haplosporidium costale
    • Ko YT, Ford SE, Fong D (1995) Characterization of the small subunit ribosomal RNA gene of the oyster parasite Haplosporidium costale. Mol Marine Biol Biotechnol 4:236-240
    • (1995) Mol Marine Biol Biotechnol , vol.4 , pp. 236-240
    • Ko, Y.T.1    Ford, S.E.2    Fong, D.3
  • 19
    • 0032810517 scopus 로고    scopus 로고
    • A PCR-ELISA method for direct detection of the oyster pathogen Haplosporidium nelsoni
    • Ko YT, Chan MMY, Ford SE, Fong D (1999) A PCR-ELISA method for direct detection of the oyster pathogen Haplosporidium nelsoni. Mar Biotechnol 1:147-154
    • (1999) Mar Biotechnol , vol.1 , pp. 147-154
    • Ko, Y.T.1    Chan, M.M.Y.2    Ford, S.E.3    Fong, D.4
  • 20
    • 0032885229 scopus 로고    scopus 로고
    • Discrimination between two Perkinsus spp. isolated from the softshell clam, Mya arenaria, by sequence analysis of two internal transcribed spacer regions and the 5.8S ribosomal RNA gene
    • Kotob SI, McLaughlin SM, van Berkum P, Faisal M (1999) Discrimination between two Perkinsus spp. isolated from the softshell clam, Mya arenaria, by sequence analysis of two internal transcribed spacer regions and the 5.8S ribosomal RNA gene. Parasitol 119:363-368
    • (1999) Parasitol , vol.119 , pp. 363-368
    • Kotob, S.I.1    McLaughlin, S.M.2    Van Berkum, P.3    Faisal, M.4
  • 21
    • 0033427819 scopus 로고    scopus 로고
    • DNA probes as potential tools for the detection of Marteilia refringens
    • Le Roux F, Audemard C, Barnaud A, Berthe F (1999) DNA probes as potential tools for the detection of Marteilia refringens. Mar Biotechnol 1:588-597
    • (1999) Mar Biotechnol , vol.1 , pp. 588-597
    • Le Roux, F.1    Audemard, C.2    Barnaud, A.3    Berthe, F.4
  • 23
    • 0033389702 scopus 로고    scopus 로고
    • Molecular characterization of QPX (Quahog Parasite Unknown), a pathogen of Mercenaria mercenaria
    • Maas PAY, Kleinschuster SJ, Dykstra MJ, Smolowitz R, Parent J (1999) Molecular characterization of QPX (Quahog Parasite Unknown), a pathogen of Mercenaria mercenaria. J Shellfish Res 18(2):561-567
    • (1999) J Shellfish Res , vol.18 , Issue.2 , pp. 561-567
    • Maas, P.A.Y.1    Kleinschuster, S.J.2    Dykstra, M.J.3    Smolowitz, R.4    Parent, J.5
  • 24
    • 0033837511 scopus 로고    scopus 로고
    • Quahog Parasite Unknown (QPX) in the northern quahog Mercenaria mercenaria (Linnaeus, 1758) and M. mercenaria var. notata from Atlantic Canada, survey results from three maritime provinces
    • MacCallum GS, McGladdery SE (2000) Quahog Parasite Unknown (QPX) in the northern quahog Mercenaria mercenaria (Linnaeus, 1758) and M. mercenaria var. notata from Atlantic Canada, survey results from three maritime provinces. J Shellfish Res 19:43-50
    • (2000) J Shellfish Res , vol.19 , pp. 43-50
    • MacCallum, G.S.1    McGladdery, S.E.2
  • 25
    • 0028991463 scopus 로고
    • A semiquantitative PCR assay for assessing Perkinsus marinus infections in the eastern oyster, Crassostrea virginica
    • Marsh AG, Gauthier JD, Vasta GR (1995) A semiquantitative PCR assay for assessing Perkinsus marinus infections in the eastern oyster, Crassostrea virginica. J Parasitol 81:577-583
    • (1995) J Parasitol , vol.81 , pp. 577-583
    • Marsh, A.G.1    Gauthier, J.D.2    Vasta, G.R.3
  • 26
    • 0000050751 scopus 로고
    • The yellow-spot disease of Tritonia diomedea Bergh, 1894 (Mollusca: Gastropoda: Nudibranchia): Encapsulation of the Thraustochytriaceous parasite by host amoebocyte
    • McLean N, Porter D (1982) The yellow-spot disease of Tritonia diomedea Bergh, 1894 (Mollusca: Gastropoda: Nudibranchia): Encapsulation of the Thraustochytriaceous parasite by host amoebocyte. J Parasitol 68:243-252
    • (1982) J Parasitol , vol.68 , pp. 243-252
    • McLean, N.1    Porter, D.2
  • 27
    • 0024241467 scopus 로고
    • The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions
    • Medlin L, Elwood HJ, Stickel S, Sogin ML (1988) The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71:491-499
    • (1988) Gene , vol.71 , pp. 491-499
    • Medlin, L.1    Elwood, H.J.2    Stickel, S.3    Sogin, M.L.4
  • 28
    • 0034141611 scopus 로고    scopus 로고
    • Genetic discrimination of sacculinid parasites (Cirripedia. Rhizocephala): Implication for control of introduced green crabs (Carcinus maenas)
    • Murphy NE, Goggin CL (2000) Genetic discrimination of sacculinid parasites (Cirripedia, Rhizocephala): Implication for control of introduced green crabs (Carcinus maenas). J Crustac Biol 20:153-157
    • (2000) J Crustac Biol , vol.20 , pp. 153-157
    • Murphy, N.E.1    Goggin, C.L.2
  • 29
    • 0001820309 scopus 로고
    • Phylum Labyrinthulomycota
    • Margulis L, Corliss JO, Melkonian M, Chapman DJ (eds). Jones and Bartlett Publishers, Boston
    • Porter D (1989) Phylum Labyrinthulomycota. In: Margulis L, Corliss JO, Melkonian M, Chapman DJ (eds) Handbook of Protoctista. Jones and Bartlett Publishers, Boston, p 388-397
    • (1989) Handbook of Protoctista , pp. 388-397
    • Porter, D.1
  • 31
    • 0032581319 scopus 로고    scopus 로고
    • Prevalence and distribution of QPX, Quahog Parasite Unknown, in hard clams Mercenaria mercenaria in Virginia, USA
    • Ragone Calvo LM, Walker JG, Burreson EM (1998) Prevalence and distribution of QPX, Quahog Parasite Unknown, in hard clams Mercenaria mercenaria in Virginia, USA. Dis Aquat Org 33:209-219
    • (1998) Dis Aquat Org , vol.33 , pp. 209-219
    • Ragone Calvo, L.M.1    Walker, J.G.2    Burreson, E.M.3
  • 32
    • 0030955515 scopus 로고    scopus 로고
    • Phylogenetic analysis of Perkinsus based on actin gene sequences
    • Reece KS, Siddall ME, Burreson EM, Graves JE (1997) Phylogenetic analysis of Perkinsus based on actin gene sequences. J Parasitol 83:417-423
    • (1997) J Parasitol , vol.83 , pp. 417-423
    • Reece, K.S.1    Siddall, M.E.2    Burreson, E.M.3    Graves, J.E.4
  • 33
    • 0000107324 scopus 로고    scopus 로고
    • Inter- and intra-specific genetic variation among Perkinsus species: Implications for species identification and development of molecular diagnostics
    • Reece KS, Brown GD, Hudson KL, Apakupakul K (2001) Inter- and intra-specific genetic variation among Perkinsus species: Implications for species identification and development of molecular diagnostics. J Shellfish Res 20:554
    • (2001) J Shellfish Res , vol.20 , pp. 554
    • Reece, K.S.1    Brown, G.D.2    Hudson, K.L.3    Apakupakul, K.4
  • 34
    • 0002794807 scopus 로고    scopus 로고
    • Observations of a protistan disease similar to QPX in Mercenaria mercenaria (hard clams) from the coast of Massachusetts
    • Smolowitz R, Leavitt D, Perkins F (1998) Observations of a protistan disease similar to QPX in Mercenaria mercenaria (hard clams) from the coast of Massachusetts. J Invertebr Pathol 71:9-25
    • (1998) J Invertebr Pathol , vol.71 , pp. 9-25
    • Smolowitz, R.1    Leavitt, D.2    Perkins, F.3
  • 35
    • 0029328316 scopus 로고
    • A sensitive and specific DNA probe for the oyster pathogen Haplosporidium nelsoni
    • Stokes NA, Burreson EM (1995) A sensitive and specific DNA probe for the oyster pathogen Haplosporidium nelsoni. J Eukaryot Microbiol 42(4):350-357
    • (1995) J Eukaryot Microbiol , vol.42 , Issue.4 , pp. 350-357
    • Stokes, N.A.1    Burreson, E.M.2
  • 36
    • 0034840161 scopus 로고    scopus 로고
    • Differential diagnosis of mixed Haplosporidium costale and Haplosporidium nelsoni infections in the eastern oyster, Crassostrea virginica, using DNA probes
    • Stokes NA, Burreson EM (2001) Differential diagnosis of mixed Haplosporidium costale and Haplosporidium nelsoni infections in the eastern oyster, Crassostrea virginica, using DNA probes. J Shellfish Res 20:207-213
    • (2001) J Shellfish Res , vol.20 , pp. 207-213
    • Stokes, N.A.1    Burreson, E.M.2
  • 37
    • 0028831292 scopus 로고
    • Detection of Haplosporidium nelsoni (Haplosporidia: Haplosporidiidae) in oysters by PCR amplification
    • Stokes NA, Siddall ME, Burreson EM (1995a) Detection of Haplosporidium nelsoni (Haplosporidia: Haplosporidiidae) in oysters by PCR amplification. Dis Aquat Org 23:145-152
    • (1995) Dis Aquat Org , vol.23 , pp. 145-152
    • Stokes, N.A.1    Siddall, M.E.2    Burreson, E.M.3
  • 38
    • 0029301912 scopus 로고
    • Small subunit ribosomal RNA gene sequence of Minchinia teredinis (Haplosporidia: Haplosporidiidae) and a specific DNA probe and PCR primers for its detection
    • Stokes NA, Siddall ME, Burreson EM (1995b) Small subunit ribosomal RNA gene sequence of Minchinia teredinis (Haplosporidia: Haplosporidiidae) and a specific DNA probe and PCR primers for its detection. J Invertebr Pathol 65:300-308
    • (1995) J Invertebr Pathol , vol.65 , pp. 300-308
    • Stokes, N.A.1    Siddall, M.E.2    Burreson, E.M.3
  • 39
    • 12244300562 scopus 로고    scopus 로고
    • Comparison of Haplosporidium nelsoni diagnostic techniques: Polymerase chain reaction outperforms histology
    • Stokes NA, Walker JG, Burreson EM (1996) Comparison of Haplosporidium nelsoni diagnostic techniques: Polymerase chain reaction outperforms histology. J Shellfish Res 15:498
    • (1996) J Shellfish Res , vol.15 , pp. 498
    • Stokes, N.A.1    Walker, J.G.2    Burreson, E.M.3
  • 41
    • 0027968068 scopus 로고
    • Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice
    • Thompson JD, Higgins DG, Gibson TJ (1994) Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680
    • (1994) Nucleic Acids Res , vol.22 , pp. 4673-4680
    • Thompson, J.D.1    Higgins, D.G.2    Gibson, T.J.3
  • 42
    • 0029146563 scopus 로고
    • Enhanced sensitivity for light and electron microscopic in situ hybridization with multiple simultaneous non-radioactive oligodeoxynucleotide probes
    • Trembleau A, Bloom FE (1995) Enhanced sensitivity for light and electron microscopic in situ hybridization with multiple simultaneous non-radioactive oligodeoxynucleotide probes. J Histochem Cytochem 43:829-841
    • (1995) J Histochem Cytochem , vol.43 , pp. 829-841
    • Trembleau, A.1    Bloom, F.E.2
  • 43
    • 0002806341 scopus 로고    scopus 로고
    • Extraction, amplification and sequencing of DNA from formaldehyde-fixed specimens
    • Vachot AM, Monnerot M (1996) Extraction, amplification and sequencing of DNA from formaldehyde-fixed specimens. Ancient Biomolecules 1:3-16
    • (1996) Ancient Biomolecules , vol.1 , pp. 3-16
    • Vachot, A.M.1    Monnerot, M.2
  • 44
    • 0028140635 scopus 로고
    • QPX (Quahaug Parasite X), a pathogen of northern quahaug Mercenaria mercenana from the Gulf of St. Lawrence, Canada
    • Whyte SK, Cawthorn RJ, McGladdery SE (1994) QPX (Quahaug Parasite X), a pathogen of northern quahaug Mercenaria mercenana from the Gulf of St. Lawrence, Canada. Dis Aquat Org 19:129-136
    • (1994) Dis Aquat Org , vol.19 , pp. 129-136
    • Whyte, S.K.1    Cawthorn, R.J.2    McGladdery, S.E.3
  • 45
    • 0033846961 scopus 로고    scopus 로고
    • A quantitative competitive polymerase chain reaction assay for the oyster pathogen Perkinsus marinus
    • Yarnall HA, Reece KS, Stokes NA, Burreson EM (2000) A quantitative competitive polymerase chain reaction assay for the oyster pathogen Perkinsus marinus. J Parasitol 86(4):827-837
    • (2000) J Parasitol , vol.86 , Issue.4 , pp. 827-837
    • Yarnall, H.A.1    Reece, K.S.2    Stokes, N.A.3    Burreson, E.M.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.