Purification and characterization of a chitinase from Serratia proteamaculans

World Journal of Microbiology and Biotechnology

Volumn 25, Issue 11, 2009, Pages 1955-1961

  Mehmood, Muhammad Aamer ^a,b   Xiao, Xiang ^b   Hafeez, Fauzia Yusuf ^a,c   Gai, Yingbao ^b   Wang, Fengping ^b  

^a NATIONAL INSTITUTE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING NIBGE   (Pakistan)

^b State Oceanic Administration   (Taiwan)

^c Department of Electrical Computer Engineering   (Pakistan)

Author keywords

Antifungal; Characterization; Chitinase; Crystalline chitin degradation; Serratia proteamaculans

Indexed keywords

AFFINITY COLUMN; ANTIFUNGAL; ASPERGILLUS NIGER; CATALYTIC DOMAINS; CHITIN-BINDING DOMAINS; CHITINASE; CHITINASE GENES; CHITINASES; COLLOIDAL CHITIN; CRYSTALLINE CHITIN DEGRADATION; ENDOCHITINASE; FUNGAL SPORES; FUSARIUM OXYSPORUMS; HYPHAL GROWTH; INCUBATION PERIODS; ISO-ELECTRIC POINTS; MODULAR STRUCTURES; N-TERMINALS; OPEN READING FRAME; PATHOGENIC FUNGI; PURIFIED ENZYME; RECOMBINANT ENZYMES; SERRATIA PROTEAMACULANS;

CHITIN; CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; CRYSTALLINE MATERIALS; DEGRADATION; ENZYMES; ESCHERICHIA COLI; FUNGI; GENE ENCODING; PURIFICATION;

ENZYME ACTIVITY;

ASPERGILLUS NIGER; ESCHERICHIA COLI; FUNGI; FUSARIUM OXYSPORUM; SERRATIA; SERRATIA PROTEAMACULANS;

EID: 70350430680     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-009-0094-3     Document Type: Article

References (31)

1. Bai Y, Souleimanov A, Smith DL (2002) An inducible activator produced by a Serratia proteamaculans strain and its soybean growth-promoting activity under greenhouse conditions. J Exp Bot 53(373):1495-1502.
2. Barboza-Corona JE, Nieto-Mazzocco E, Velazquez-Robledo R, Salcedo-Hernandez R, Bautista M, Jimenez B, Ibarra JE (2003) Cloning, sequencing, and expression of the chitinase gene chiA74 from Bacillus thuringiensis. Appl Environ Microbiol 69(2):1023-1029.
3. Brurberg MB, Nes IF, Eijsink VG (1996) Comparative studies of chitinases A and B from Serratia marcescens. Microbiology 142(7):1581-1589.
4. Chernin L, Ismailov Z, Haran S, Chet I (1995) Chitinolytic Enterobacter agglomerans antagonistic to fungal plant pathogens. Appl Environ Microbiol 61(5):1720-1726.
5. Chernin LS, De la Fuente L, Sobolev V, Haran S, Vorgias CE, Oppenheim AB, Chet I (1997) Molecular cloning, structural analysis, and expression in Escherichia coli of a chitinase gene from Enterobacter agglomerans. Appl Environ Microbiol 63(3):834-839.
6. Chet I, Inbar J (1994) Biological control of fungal pathogens. Appl Biochem Biotechnol 48(1):37-43.
7. Frankowski J, Lorito M, Scala F, Schmid R, Berg G, Bahl H (2001) Purification and properties of two chitinolytic enzymes of Serratia plymuthica HRO-C48. Arch Microbiol 176(6):421-426.
8. Ghosh M (2006) Antifungal properties of haem peroxidase from Acorus calamus. Annals Bot 98(6):1145-1153.
9. Gomes RC, Semedo LT, Soares RM, Linhares LF, Ulhoa CJ, Alviano CS, Coelho RR (2001) Purification of a thermostable endochitinase from Streptomyces RC1071 isolated from a cerrado soil and its antagonism against phytopathogenic fungi. J Appl Microbiol 90(4):653-661.
10. Hardt M, Laine RA (2004) Mutation of active site residues in the chitin-binding domain ChBDChiA1 from chitinase A1 of Bacillus circulans alters substrate specificity: Use of a green fluorescent protein binding assay. Arch Biochem Biophys 426(2):286-297.
11. Hashimoto M, Honda Y, Nikaidou N, Fukamizo T, Watanabe T (2000) Site-directed mutagenesis of Asp280 suggests substrate-assisted catalysis of chitinase A1 from Bacillus circulans WL-12. J Biosci Bioeng 89(1):100-102.
12. Horn SJ, Sikorski P, Cederkvist JB, Vaaje-Kolstad G, Sorlie M, Synstad B, Vriend G, Varum KM, Eijsink VG (2006a) Costs and benefits of processivity in enzymatic degradation of recalcitrant polysaccharides. Proc Natl Acad Sci USA 103(48):18089-18094.
13. Horn SJ, Sorbotten A, Synstad B, Sikorski P, Sorlie M, Varum KM, Eijsink VG (2006b) Endo/exo mechanism and processivity of family 18 chitinases produced by Serratia marcescens. FEBS J 273(3):491-503.
14. Jones JD, Grady KL, Suslow TV, Bedbrook JR (1986a) Isolation and characterization of genes encoding two chitinase enzymes from Serratia marcescens. EMBO J 5(3):467-473.
15. Jones JDG, Grady KL, Suslow TV, Bedrook JR (1986b) Isolation and characterization of genes encoding two chitinases enzymes from Serratia marcescens. EMBO J 5:467-473.
16. Kirubakaran SI, Sakthivel N (2007) Cloning and overexpression of antifungal barley chitinase gene in Escherichia coli. Protein Expr Purif 52(1):159-166.
17. Kobayashi DY, Reedy RM, Bick J, Oudemans PV (2002) Characterization of a chitinase gene from Stenotrophomonas maltophilia strain 34S1 and its involvement in biological control. Appl Environ Microbiol 68(3):1047-1054.
18. Li YC, Chang CT, Hsiao ES, Hsu JS, Huang JW, Tzen JT (2003) Purification and characterization of an antifungal chitinase in jelly fig (Ficus awkeotsang) achenes. Plant Cell Physiol 44(11):1162-1167.
19. Li Q, Xiao X, Wang F (2007) Screening of genes involved in chitinase production in Aeromonas caviae CB101 via transposon mutagenesis. J Appl Microbiol 102(3):640-649.
20. Miller G (1959) Use of dinitrosalicylic acid reagent for detection of reducing sugar. Anal Chem 31:426-428.
21. Neu HC, Heppel LA (1965) The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem 240(9):3685-3692.
22. Pan H, Wei Y, Xin F, Zhou M, Zhang S (2006) Characterization and biocontrol ability of fusion chitinase in Escherichia coli carrying chitinase cDNA from Trichothecium roseum. Z Naturforsch [C] 61(5-6):397-404.
23. Reyes-Ramírez A, Escudero-Abarca BI, Aguilar-Uscanga G, Hayward-Jones PM, Barboza-Corona JE (2004) Antifungal activity of Bacillus thuringiensis chitinase and its potential for the biocontrol of phytopathogenic fungi in soybean seeds. J Food Sci 69:131-134.
24. Shimahara K, Takiguchi Y (1988) Preparation of crustacean chitin. Methods Enzymol 161:417-423.
25. Suzuki K, Sugawara N, Suzuki M, Uchiyama T, Katouno F, Nikaidou N, Watanabe T (2002) Chitinases A, B, and C1 of Serratia marcescens 2170 produced by recombinant Escherichia coli: Enzymatic properties and synergism on chitin degradation. Biosci Biotechnol Biochem 66(5):1075-1083.
26. Uchiyama T, Katouno F, Nikaidou N, Nonaka T, Sugiyama J, Watanabe T (2001) Roles of the exposed aromatic residues in crystalline chitin hydrolysis by chitinase A from Serratia marcescens 2170. J Biol Chem 276(44):41343-41349.
27. Vaaje-Kolstad G, Horn SJ, van Aalten DM, Synstad B, Eijsink VG (2005) The non-catalytic chitin-binding protein CBP21 from Serratia marcescens is essential for chitin degradation. J Biol Chem 280(31):28492-28497.
28. Wang FP, Li Q, Zhou Y, Li MG, Xiao X (2003) The C-terminal module of Chi1 from Aeromonas caviae CB101 has a function in substrate binding and hydrolysis. Proteins 53(4):908-916.
29. Watanabe T, Kimura K, Sumiya T, Nikaidou N, Suzuki K, Suzuki M, Taiyoji M, Ferrer S, Regue M (1997) Genetic analysis of the chitinase system of Serratia marcescens 2170. J Bacteriol 179(22):7111-7117.
30. Wu ML, Chuang YC, Chen JP, Chen CS, Chang MC (2001) Identification and characterization of the three chitin-binding domains within the multidomain chitinase Chi92 from Aeromonas hydrophila JP101. Appl Environ Microbiol 67(11):5100-5106.
31. Zhang Z, Yuen GY, Sarath G, Penheiter AR (2000) Chitinases from the plant disease biocontrol agent, Stenotrophomonas maltophilia C3. Biol Control 91(2):205-211.