Cloning and overexpression of antifungal barley chitinase gene in Escherichia coli

Protein Expression and Purification

Volumn 52, Issue 1, 2007, Pages 159-166

  Kirubakaran, S Isaac ^a   Sakthivel, N ^a  

^a PONDICHERRY UNIVERSITY   (India)

Author keywords

Antifungal activity; Barley chitinase; Escherichia coli expression; Pathogenesis related proteins

Indexed keywords

ANTIFUNGAL AGENT; CHITINASE; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

ARTICLE; BARLEY; BOTRYTIS; DRUG EFFECT; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MOLECULAR CLONING; PLANT DISEASE; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ANTIFUNGAL AGENTS; BOTRYTIS; CHITINASE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HORDEUM; MICROBIAL SENSITIVITY TESTS; PLANT DISEASES; PLANT PROTEINS; RECOMBINANT PROTEINS;

ALTERNARIA; BIPOLARIS ORYZAE; BOTRYOTINIA FUCKELIANA; COCHLIOBOLUS LUNATUS; ESCHERICHIA COLI; HORDEUM VULGARE SUBSP. VULGARE; HYPHOMYCETES; NICOTIANA TABACUM; PESTALOTIA; PUNCTUM BLANDIANUM; THANATEPHORUS CUCUMERIS; TRIFOLIUM;

EID: 33845960213     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.08.012     Document Type: Article

References (35)

1. Bell J.N., Ryder T.B., Wingate V.P.M., Bailey J.A., and Lamb C.J. Differential accumulation of plant defense gene transcripts in a compatible plant-pathogen interaction. Mol. Cell. Biol. 6 (1986) 1615-1623
2. Benhamou N., Joosten M.H.A.J., and De Wit P.J.G.M. Subcellular localization of chitinase and of potential substrate in tomato root tissue infected by Fusarium oxysporum f sp. radicis-lycopersici. Plant Physiol. 92 (1990) 1108-1120
3. Bol J.F., Linthorst H.J.M., and Cornelissen B.J.C. Plant pathogenesis-related proteins induced by virus infection. Annu. Rev. Phytopathol. 28 (1990) 113-138
4. Boller T. Ethylene and the regulation of antifungal hydrolases in plants. Oxf. Surv. Plant Mo1. Cell Biol. 5 (1988) 145-174
5. Bowles D. Defense-related proteins in higher plants. Annu. Rev. Biochem. 59 (1990) 873-907
6. Bowyer P. Plant disease caused by fungi: phytopathogenicity. In: Oliver R.P., and Schweizer M. (Eds). Molecular Fungal Biology (1999), Cambridge University Press, Cambridge
7. Brunner F., Stintzi A., Fritig B., and Legrand M. Substrate specificities of tobacco chitinases. Plant J. 14 (1998) 225-234
8. Chen Z.Y., Brown R.L., Lax A.R., Cleveland T.E., and Russin J.S. Inhibition of plant-pathogenic fungi by a corn trypsin inhibitor overexpressed in Escherichia coli. Appl. Environ. Microbiol. 65 (1999) 1320-1324
9. Cohen-Kupiec R., and Chet I. The molecular biology of chitin digestion. Curr. Opin. Biotechnol. 9 (1998) 270-277
10. Collinge D.B., Kragh K.M., Mikkelesen J.D., Nielsen K.K., Rasmussen U., and Vad K. Plant chitinase. Plant J. 3 (1993) 31-40
11. Dore I., Legrand M., Cornelissen B.J.C., and Bol J.F. Subcellular localization of acidic and basic PR proteins in tobacco mosaic virus infected tobacco. Arch. Virol. 120 (1991) 97-107
12. Hahlbrock K., and Griscbach H. Enzymic controls in the biosynthesis of lignin and flavonoids. Ann. Rev. Plant. Physiol. 30 (1979) 105-130
13. Huynh Q., Hironaka C., Levine E., Smith C., Borgmeyer J., and Shah D. Antifungal proteins from plants: Purification, molecular cloning and antifungal properties of chitinases from maize seed. J. Biol. Chem. 267 (1992) 6635-6640
14. Ignatius S.M.J., Huang J.K., Chopra R.K., and Muthukrishnan S. Isolation and characterization of barley chitinase genomic clone-expression in powdery mildew infected barley. J. Plant Biochem. Biotechnol. 3 (1994) 91-95
15. Johnson L.F., and Curl E.A. Methods for research on the ecology of soil-borne plant pathogens (1972), Burgess Publishing Company, Minnesota p. 241
16. Keefe D., Hinz U., and Meins F. The effect of ethylene on the cell-type specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves. Planta 182 (1990) 43-51
17. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
18. Leah R., Tommerup H., Svendsen I., and Mundy J. Biochemical and molecular characterization of three barley seed proteins with antifungal activity. J. Biol. Chem. 66 (1991) 1464-1573
19. Linthorst H.J.M. Pathogenesis-related proteins of plants. Crit. Rev. Plant Sci. 10 (1991) 123-150
20. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
21. Marston F.A.O. The purification of eukaryotic polypeptides synthesized in Escherichia coli. Biochem. J. 240 (1986) 1-12
22. Melchers L.S., Ponstein A.S., Sela-Buurlage M.B., Vloemans S.A., and Cornelissen B.J.C. In vitro anti-microbial activities of defense proteins and biotechnology. In: Fritig B., and Legrand M. (Eds). Mechanisms of Plant Defense (1993), Kluwer Academic Publishers, Dordrecht, The Netherlands 401-410
23. Mincoff P.C., Garcia Cortez D.A., Ueda-Nakamura T., Vataru Nakamura C., and Filho B.P.D. Isolation and characterization of a 30 kDa antifungal protein from seeds of Sorghum bicolor. Res. Microbiol. 157 (2006) 326-332
24. Neuhaus J.M., Fritig B., Linthorst H.J.M., and Meins F.J. A revised nomenclature for chitinase genes. Plant Mol. Biol. Rep. 14 (1996) 102-104
25. Roberts W.K., and Selitrennikoff C.P. Plant and bacterial chitinases differ in antifungal activity. J. Gen. Microbiol. 134 (1988) 169-176
26. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual (1989), Cold Spring Harbor, Cold Spring Harbor Press, New York
27. Schein C.H. Production of soluble recombinant proteins in bacteria. Bio/Technology 7 (1989) 1141-1149
28. Schickler H., and Chet I. Heterologous chitinase gene expression to improve plant defense against phytopathogenic fungi. J. Ind. Microbiol. Biotechnol. 19 (1997) 196-201
29. Schultze M., Staehelin C., Brunner F., Genetet I., Legrand M., Fritig B., Kondorosi E., and Kondorosi A. Plant chitinase/lysozyme isoforms show distinct substrate specificity and cleavage site preference towards lipochitooligosaccharide Nod signals. Plant J. 16 (1998) 571-580
30. Seetharaman K., Whitehead E., Keller N.P., Waniskar R.D., and Rooney L.W. In vitro activity of sorghum seed antifungal proteins against grain mold pathogens. J. Agric. Food Chem. 45 (1997) 3666-3671
31. Shirano Y., and Shibata D. Low temperature cultivation of Escherichia coli carrying a rice lipoxygenase L-2 cDNA produces a soluble and active enzyme at a high level. FEBS Lett. 271 (1990) 128-130
32. Subroto T., Sufiati S., and Beintema J.J. Papaya (Carica papaya) lysozyme is a member of the family 19 (basic, class II) chitinases. J. Mol. Evol. 49 (1999) 819-821
33. Towbin H., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 79 (1979) 4350-4354
34. Verburg J.G., and Huynh Q.K. Purification and characterization of an antifungal chitinase from Arapidopsis thaliana. Plant Physiol. 95 (1991) 450-455
35. Ye X., and Ng T.B. A chitinase with antifungal activity from the mung bean. Protein Express. Purif. 40 (2005) 230-236