Evaluation of extraction and purification methods for obtaining PCR-amplifiable DNA from aged refuse for microbial community analysis

World Journal of Microbiology and Biotechnology

Volumn 25, Issue 11, 2009, Pages 2043-2051

  He, Yan ^a,b   Zhao, Youcai ^a   Zhou, Gongming ^a   Huang, Minsheng ^b  

^a TONGJI UNIVERSITY   (China)

^b EAST CHINA NORMAL UNIVERSITY   (China)

Author keywords

Aged refuse; DGGE; DNA extraction; PCR

Indexed keywords

16S RDNA; AGED REFUSE; ARCHAEAL; DENATURING GRADIENT GEL ELECTROPHORESIS; DGGE; DNA EXTRACTION; DNA YIELDS; FRAGMENT SIZES; GENETIC DIVERSITY; HIGH QUALITY; MICROBIAL COMMUNITY ANALYSIS; ORTHOGONAL EXPERIMENT; PCR; PRE-TREATMENT; PRE-WASHING; PROTEINASE K; PURIFICATION METHOD; SEPHADEX; SPIN COLUMN;

DEXTRAN; DIFFUSERS (OPTICAL); DNA; ELECTROPHORESIS; GENES; ION EXCHANGE; PURIFICATION; SOILS; SPIN DYNAMICS;

NUCLEIC ACIDS;

ARCHAEA; BACTERIA (MICROORGANISMS);

EID: 70350426874     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-009-0106-3     Document Type: Article

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