Evaluation of a Multiplex PCR System for Simultaneous Detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in Foods and in Food Subjected to Freezing

Foodborne Pathogens and Disease

Volumn 6, Issue 1, 2009, Pages 81-89

  Kawasaki, Susumu ^a   Fratamico, Pina M ^b   Horikoshi, Naoko ^c   Okada, Yukio ^c   Takeshita, Kazuko ^c   Sameshima, Takashi ^c   Kawamoto, Shinichi ^a  

^a NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

^b EASTERN REGIONAL RESEARCH CENTER   (United States)

^c Basic Research Laboratory   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ESCHERICHIA COLI O157; FOOD CONTAMINATION; FOOD CONTROL; FOOD FREEZING; HUMAN; ISOLATION AND PURIFICATION; LISTERIA MONOCYTOGENES; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SAFETY; SALMONELLA; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; STANDARD; VALIDATION STUDY;

CONSUMER PRODUCT SAFETY; ESCHERICHIA COLI O157; FOOD CONTAMINATION; FOOD MICROBIOLOGY; FROZEN FOODS; HUMANS; LISTERIA MONOCYTOGENES; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SALMONELLA; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY;

EID: 70350297697     PISSN: 15353141     EISSN: 15567125     Source Type: Journal    
DOI: 10.1089/fpd.2008.0153     Document Type: Article

References (16)

1. Buzby JC. Children and microbial foodborne illness. Food Review 2000; 24: 32–37.
2. Croci L, Delibato E, Volpe G, et al. Comparison of PCR, electrochemical enzyme-linked immunosorbent assays, and the standard culture method for detecting salmonella in meat products. Appl Environ Microbiol 2004; 70:1393–1396.
3. Fukushima H, Katsube K, Hata Y, et al. Rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time PCR. Appl Environ Microbiol 2007; 73: 92–100.
4. Kawasaki S, Horikoshi N, Okada Y, et al. Multiplex PCR for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in meat samples. J Food Prot 2005; 68:551–556.
5. Kimura B, Kawasaki S, Fujii T, et al. Evaluation of TaqMan PCR assay for detecting Salmonella in raw meat and shrimp. J Food Prot 1999; 62:329–335.
6. McLauchlin J. The relationship between Listeria and listeriosis. Food Control 1996; 7:187–193.
7. Mead PS, Slutsker L, Dietz V, et al. Food-related illness and death in the United States. Emerg Infect Dis 1999; 5: 607–625.
8. Oberst RD, Hays MP, Bohra LK, et al. PCR-based DNA amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5′ nuclease (TaqMan) assay. Appl Environ Microbiol 1998; 64:3389–3396.
9. Renault T, LeDeuff RM, Lipart C, et al. Development of a PCR procedure for the detection of a herpes-like virus infecting oysters in France. J Virol Methods 2000; 88: 41–50.
10. Schlosser W, Hogue A, Ebel E, et al. Analysis of Salmonella serotypes from selected carcasses and raw ground products sampled prior to implementation of the pathogen reduction; hazard analysis and critical control point final rule in the US. Int J Food Microbiol 2000; 30:107–111.
11. Sharma VK, Dean-Nystrom EA, and Casey TA. Semi-automated fluorogenic PCR assays (TaqMan) for rapid detection of Escherichia coli O157:H7 and other Shiga toxigenic E. coli. Mol Cell Probes 1999; 13:291–302.
12. Thomas EJ, King RK, Burchal J, et al. Sensitive and specific detection of Listeria monocytogenes in milk and ground beef with the polymerase chain reaction. Appl Environ Microbiol 1991; 57:2576–2580.
13. Uyttendaele M, Grangette C, Rogerie F, et al. Influence of cold stress on the preliminary enrichment time needed for detection of enterohemorrhagic Escherichia coli in ground beef by PCR. Appl Environ Microbiol 1998; 64:1640–1643.
14. Wells EV, Boulton M, Hall W, et al. Reptile-associated salmonellosis in preschool-aged children in Michigan, January 2001–June 2003. Clin Infect Dis 2004; 39:687–691.
15. Wolffs PFG, Glencross K, Thibaudeau R, et al. Direct quantification and detection of salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR. Appl Environ Microbiol 2006; 72: 3896–3900.
16. Wolffs P, Knutsson R, Norling B, et al. Rapid quantification of Yersinia enterocolitica in pork samples by a novel sample preparation method, flotation, prior to real-time PCR. J Clin Microbiol 2004; 42:1042–1047.