Delivery of short hairpin RNAs by transkingdom RNA interference modulates the classical ABCB1-mediated multidrug-resistant phenotype of cancer cells

Cell Cycle

Volumn 8, Issue 20, 2009, Pages 3349-3354

  Krühn, Andrea ^a   Wang, Aijin ^b   Fruehauf, Johannes H ^b,c   Lage, Hermann ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b Cequent Pharmaceuticals Inc   (United States)

^c BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

ABCB1; Cancer; E. coli; Gene therapy; MDR1; Multidrug resistance; RNA interference

Indexed keywords

DAUNORUBICIN; MESSENGER RNA; MULTIDRUG RESISTANCE PROTEIN 1; SHORT HAIRPIN RNA;

ANIMAL CELL; ARTICLE; CANCER CELL; CARCINOMA CELL; CONTROLLED STUDY; CYTOLYSIS; CYTOTOXICITY; DOWN REGULATION; DRUG ACCUMULATION; DRUG DELIVERY SYSTEM; DRUG RESISTANCE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE DELIVERY SYSTEM; GENE EXPRESSION; GENE SILENCING; HUMAN; HUMAN CELL; INTERNALIZATION; MOUSE; NONHUMAN; PHENOTYPE; PROTEIN EXPRESSION; RNA INTERFERENCE; TRANSKINGDOM RNA INTERFERENCE;

EID: 70350241634     PISSN: 15384101     EISSN: 15514005     Source Type: Journal    
DOI: 10.4161/cc.8.20.9845     Document Type: Article

References (16)

1. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001; 411:494-498 (Pubitemid 32494397)
2. Sanguino A, Lopez-Berestein G, Sood AK. Strategies for in vivo siRNA delivery in cancer. Mini Rev Med Chem 2008; 8:248-255 (Pubitemid 351611827)
3. Xiang S, Fruehauf J, Li CJ. Short hairpin RNA-expressing bacteria elicit RNA interference in mammals. Nat Biotechnol 2006; 24:697-702. (Pubitemid 43882121)
4. Lage H. An overview of cancer multidrug resistance: a still unsolved problem. Cell Mol Life Sci 2008; 65:3145-3167
5. Lage H. Therapeutic potential of RNA interference in drug-resistant cancers. Fut Oncol 2009; 5:169-185
6. Dietel M, Arps H, Lage H, Niendorf A. Membrane vesicle formation due to acquired mitoxantrone resistance in human gastric carcinoma cell line EPG85-257. Cancer Res 1990; 50:6100-6106 (Pubitemid 20331427)
7. Lage H. Molecular analysis of therapy resistance in gastric cancer. Dig Dis 2003; 21:326-338 (Pubitemid 38209790)
8. Stege A, Priebsch A, Nieth C, Lage H. Stable and complete overcoming of MDR1/P-glycoprotein-mediated multidrug resistance in human gastric carcinoma cells by RNA interference. Cancer Gene Ther 2004; 11:699-706. (Pubitemid 39488467)
9. Jagielski M, Zaleska M, Kaluzewski S, Polna I. Applicability of DAPI for the detection of Mycoplasms in cell cultures [Article in Polish]. Med Dosw Mikrobiol 1976; 28:161-173
10. Stein U, Walther W, Stege A, Kaszubiak A, Fichtner I, Lage H. Complete in vivo reversal of the multidrug resistance phenotype by jet-injection of anti-MDR1 short hairpin RNA-encoding plasmid DNA. Mol Ther 2008; 16:178-186
11. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001; 25:402-408 (Pubitemid 34164012)
12. Kaszubiak A, Kupstat A, Müller U, Hausmann R, Holm PS, Lage H. Regulation of MDR1 gene expression in multidrug-resistant cancer cells is independent from YB-1. Biochem Biophys Res Comm 2007; 357:295-301. (Pubitemid 46584653)
13. Kaszubiak A, Holm PS, Lage H. Overcoming the classical multidrug resistance phenotype by adenoviral delivery of anti-MDR1 short hairpin RNAs and ribozymes. Int J Oncol 2007; 31:419-430
14. Kowalski P, Surowiak P, Lage H. Reversal of different drug-resistant phenotypes by an autocatalytic multitarget multiribozyme directed against the transcripts of the ABCtransporters MDR1/P-gp, MRP2, and BCRP. Mol Ther 2005; 11:508-522 (Pubitemid 40353109)
15. Nieth C, Priebsch A, Stege A, Lage H. Modulation of the classical multidrug resistance (MDR) phenotype by RNA interference (RNAi). FEBS Lett 2003; 545:144-150
16. Keates AC, Fruehauf JH, Xiang S, Parker PD, Li CJ. Cequent Pharmaceuticals, Inc.: the biological pitcher for RNAi therapeutics. Pharmacogenomics 2007; 8:867-871