Molecular analysis of Leptospira spp. isolated from humans by restriction fragment length polymorphism, real-time PCR and pulsed-field gel electrophoresis

FEMS Microbiology Letters

Volumn 300, Issue 2, 2009, Pages 174-179

  Turk, Nenad ^a   Milas, Zoran ^a   Mojcec, Vesna ^a   Ruzic Sabljic, Eva ^b   Staresina, Vilim ^a   Stritof, Zrinka ^a   Habus, Josipa ^a   Postic, Daniele ^c  

^a UNIVERSITY OF ZAGREB   (Croatia)

^b UNIVERSITY OF LJUBLJANA   (Slovenia)

^c INSTITUT PASTEUR   (France)

Author keywords

Leptospira; Leptospirosis; Pulsed field gel electrophoresis; Real time PCR; Restriction fragment length polymorphism

Indexed keywords

GENOMIC DNA;

ARTICLE; BACTERIAL GENETICS; BACTERIAL STRAIN; CROATIA; GENE IDENTIFICATION; GENETIC ANALYSIS; LEPTOSPIRA; NONHUMAN; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

BACTERIAL TYPING TECHNIQUES; CLUSTER ANALYSIS; CROATIA; DNA FINGERPRINTING; DNA, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; GENOTYPE; HUMANS; LEPTOSPIRA; LEPTOSPIROSIS; MOLECULAR EPIDEMIOLOGY; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SEROTYPING;

LEPTOSPIRA; LEPTOSPIRA BORGPETERSENII; LEPTOSPIRA INTERROGANS; LEPTOSPIRA KIRSCHNERI;

EID: 70350238526     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2009.01776.x     Document Type: Article

References (25)

1. Brenner DJ, Kaufmann AF, Sulzer KR, Steigerwalt AG, Rogers FC Weyant RS (1999) Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. Int J Syst Bacteriol 49 : 839 858.
2. Collares-Pereira M, Korver H, Cao Thi BV, Santos-Reis M, Bellenger E, Baranton G Terpstra WJ (2000) Analysis of Leptospira isolates from mainland Portugal and the Azores islands. FEMS Microbiol Lett 185 : 181 187.
3. Dikken H Kmety E (1978) Serological typing methods of leptospires. Methods in Microbiology, Vol. 11 (Bergan T Norris JR, eds), pp. 259 307. Academic Press, New York.
4. Ellinghausen HC McCullough WG (1965) Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am J Vet Res 26 : 45 51.
5. Ellis WA (1995) International committee on systematic bacteriology subcommittee on the taxonomy of Leptospira. Minutes of meetings. Int J Syst Bacteriol 45 : 872 874.
6. Faine S, Adler B, Bolin C Pérolat P (1999) Leptospira and Leptospirosis, 2nd edn. MediScience, Melbourne, Australia.
7. Galloway R Levett PN (2008) Evaluation of a modified pulsed-field gel electrophoresis approach for the identification of Leptospira serovars. Am J Trop Med Hyg 78 : 628 632.
8. Herrmann JL, Baril C, Bellenger E, Pérolat P, Baranton G Saint Girons I (1991) Genome conservation in isolates of Leptospira interrogans. J Bacteriol 173 : 7582 7588.
9. Herrmann JL, Bellenger E, Pérolat P, Baranton G Saint Girons I (1992) Pulsed-field gel electrophoresis of NotI digests of leptospiral DNA; a new rapid method of serovar identification. J Clin Microbiol 30 : 1696 1702.
10. Hookey JV (1993) Characterization of Leptospiraceae by 16S restriction fragment length polymorphisms. J Gen Microbiol 139 : 1681 1689.
11. Johnson RC Harris WG (1967) Differentiation of pathogenic and saprophytic leptospires. Growth at low temperatures. J Bacteriol 94 : 27 31.
12. Korthof G (1932) Experimentelles Schlammfieber beim Menschen. Zbl Bakt Hyg., I Abt 125 : 429 434.
13. Levett PN (2004) Leptospirosis: a forgotten zoonosis? Clin Applied Immunol Rev 4 : 435 448.
14. Levett PN, Morey RE, Galloway RL Steigerwalt AG (2006) Leptospira broomii sp. Nov., isolated from humans with leptospirosis. Int J Syst Evol Micr 56 : 671 673.
15. Lewett PN, Morey RE, Galloway RE, Turner DE, Steigerwalt AG Mayer LW (2005) Detection of pathogenic leptospires by real-time quantitative PCR. J Med Microbiol 54 : 45 49.
16. Mérien F, Amouriaux P, Pérolat P, Baranton G Saint Girons I (1992) Polymerase chain reaction for detection of Leptospira spp. in clinical samples. J Clin Microbiol 30 : 2219 2224.
17. Merien F, Portnoi D, Bourhy P, Charavay F, Berlioz-Arthaud A Baranton G (2005) A rapid and quantitative method for the detection of Leptospira species in human leptospirosis. FEMS Microbiol Lett 249 : 139 147.
18. Pérolat P, Grimont F, Regnault B, Grimont PAD, Fournie E, Thevenet H Baranton G (1990) rRNA gene restriction patterns of Leptospira: a molecular typing system. Res Microbiol 141 : 159 171. (Pubitemid 20118717)
19. Pérolat P, Lecuyer I, Postic D Baranton G (1993) Diversity of ribosomal DNA fingerprints of Leptospira serovars provides a database for subtyping and species assignation. Res Microbiol 144 : 5 15.
20. Pérolat P, Merien F, Ellis WA Baranton G (1994) Characterization of Leptospira isolates from serovar hardjo by ribotyping, arbitrarily primed PCR and mapped restriction site polymorphism. J Clin Microbiol 32 : 1949 1957.
21. Ramadass P, Jarvis BDW, Corner CJ, Penny D Marshall RB (1992) Genetic characterization of pathogenic Leptospira species by DNA hybridization. Int J Syst Bacteriol 42 : 215 219.
22. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH Swaminathan B (1995) Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33 : 2233 2239.
23. Turk N, Milas Z, Margaletic J, Staresina V, Slavica A, Riquelme-Sertour N, Bellenger E, Baranton G Postic D (2003) Molecular characterisation of Leptospira spp. strains isolated from small rodents in Croatia. Epidemiol Infect 130 : 159 166.
24. Yasuda PH, Steigerwalt AG, Sulzer KR, Kaufmann AF, Rogers F Brenner DJ (1987) Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species. Int J Syst Bacteriol 37 : 407 415.
25. Zuerner RL Bolin CA (1990) Nucleic acid probe characterizes Leptospira interrogans serovars by restriction fragment length polymorphisms. Vet Microbiol 24 : 355 366.