A rapid and quantitative method for the detection of Leptospira species in human leptospirosis

FEMS Microbiology Letters

Volumn 249, Issue 1, 2005, Pages 139-147

  Merien, Fabrice ^a   Portnoi, Denis ^b   Bourhy, Pascale ^b   Charavay, Françoise ^a   Berlioz Arthaud, Alain ^a   Baranton, Guy ^b  

^a Institut Pasteur de Nouvelle Caledonie Noumea   (New Caledonia)

^b INSTITUT PASTEUR   (France)

Author keywords

Leptospira; Leptospirosis; Real time PCR

Indexed keywords

DNA;

ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BLOOD; CONTROLLED STUDY; DATA ANALYSIS; GENE AMPLIFICATION; GENOME ANALYSIS; HUMAN; LEPTOSPIRA; LEPTOSPIROSIS; MELTING POINT; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SERUM; SYMPTOMATOLOGY;

ADOLESCENT; ADULT; AGED; CHILD; DNA PRIMERS; DNA, BACTERIAL; FEMALE; HUMANS; LEPTOSPIRA; LEPTOSPIROSIS; MALE; MIDDLE AGED; ORGANIC CHEMICALS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

LEPTOSPIRA;

EID: 22144448856     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2005.06.011     Document Type: Article

References (18)

1. R. Plank, and D. Dean Overview of the epidemiology, microbiology, and pathogenesis of Leptospira spp. in humans Microb. Infect. 2 2000 1265 1276
2. P.N. Levett Leptospirosis Clin. Microbiol. Rev. 14 2001 296 326
3. J. Truccolo, O. Serais, F. Merien, and P. Perolat Following the course of human leptospirosis: evidence of a critical threshold for the vital prognosis using a quantitative PCR assay FEMS Microbiol. Lett. 204 2001 317 321
4. P.H. Yasuda, A.G. Steigerwalt, K.R. Sulzer, A.F. Kaufmann, F.C. Rogers, and D.J. Brenner Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species Int. J. Syst. Bacteriol. 37 1987 407 415
5. D.J. Brenner, A.F. Kaufmann, K.R. Sulzer, A.G. Steigerwalt, F.C. Rogers, and R.S. Weyant Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies Int. J. Syst. Bacteriol. 49 1999 839 858
6. F. Merien, P. Amouriaux, P. Perolat, G. Baranton, and I. Girons Saint Polymerase chain reaction for detection of Leptospira spp. in clinical samples J. Clin. Microbiol. 30 1992 2219 2224
7. D. Postic, N. Riquelme Sertour, F. Merien, P. Perolat, and G. Baranton Interest of partial 16S rDNA gene sequences to resolve heterogeneities between Leptospira collections: application to L. meyeri Res. Microbiol. 151 2000 333 341
8. J. Truccolo, F. Charavay, F. Merien, and P. Perolat Quantitative PCR to evaluate ampicillin, ofloxacin, and deoxycycline for treatment of experimental leptospirosis Antimicrob. Agents. Chemother. 46 2002 848 853
9. R. Boom, C.J.A. Sol, M.M.M. Salimans, C.L. Jansen, P.M.E. Wertheim-van Dillen, and J. van der Noordaa Rapid and simple method for purification of nucleic acids J. Clin. Microbiol. 28 1990 495 503
10. S.X. Ren, G. Fu, X.G. Jiang, R. Zeng, Y.G. Miao, H. Xu, Y.X. Zhang, H. Xiong, G. Lu, L. Lu, F. Lu, H.Q. Jiang, J. Jia, Y.F. Tu, J.X. Jiang, W.Y. Gu, Y.Q. Zhang, Z. Cai, H.H. Sheng, H.F. Yin, Y. Zhang, G.F. Zhu, M. Wan, H.L. Huang, Z. Qian, S.Y. Wang, W. Ma, Z.J. Yao, Y. Shen, B.Q. Qiang, Q.C. Xia, X.K. Guo, A. Danchin, I. Girons, R.L. Somerville, Y.M. Weng, M.H Shi, Z. Chen, J.G. Xu, and G.P. Zhao Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing Nature 422 2003 88 893
11. D.J. Brenner, A.C. McWhorter, J.K. Knutson, and A.G. Steigerwalt Leete Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds J. Clin. Microbiol. 15 1982 1133 1140
12. A. Berlioz-Arthaud, and T. Kiedrzynski Survey on leptospirosis in the Pacific Inform'Action 16 2003 5 8 (PPHSN quarterly bulletin, http://www.spc.int/phs )
13. F. Merien, G. Baranton, and P. Perolat Comparison of polymerase chain reaction with microagglutination test and culture for diagnosis of leptospirosis J. Infect. Dis. 172 1995 281 285
14. L.D. Smythe, I.L. Smith, G.A. Smith, M.F. Dohnt, M.L. Symonds, L.J. Barnett, and D.B. McKay A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp BMC Infect. Dis. 2 2002 13 19
15. A.E. Bal, C. Gravekamp, R.A. Hartskeerl, J. de Meza-Brewster, H. Korver, and W.J. Terpstra Detection of leptospires in urine by PCR for early diagnosis of leptospirosis J. Clin. Microbiol. 32 1994 1894 1898
16. F. Merien, P. Perolat, E. Mancel, D. Persan, and G. Baranton Detection of Leptospira DNA by polymerase chain reaction in aqueous humor of a patient with unilateral uveitis J. Infect. Dis. 168 1993 1335 1336
17. P.N. Levett, R.E. Morey, R.E. Galloway, D.E. Turner, A.G. Steigerwalt, and L.W. Mayer Detection of pathogenic leptospires by real-time quantitative PCR J. Med. Microbiol. 54 2005 45 49
18. M.A.A. Oliveira, O.L. Caballero, E. Neto, M.C. Koury, A.J. Romanha, J. Carvalho, R.A. Hartskeerl, and A.J.G. Simpson Dias Use of nondenaturing silver-stained polyacrylamide gel analysis of polymerase chain reaction amplification products for the differential diagnosis of Leptospira interrogans infection Diagn. Microbiol. Infect. Dis. 22 1995 343 348