Chromatographic analysis of conformationally changed insulin and its cytotoxic effect on PC12 cells

Journal of Health Science

Volumn 55, Issue 5, 2009, Pages 825-831

  Iwasa, Sumiko ^a   Enomoto, Akina ^a   Onoue, Satomi ^a,b   Nakai, Minako ^a   Yajima, Takehiko ^a   Fukushima, Takeshi ^a  

^a TOHO UNIVERSITY   (Japan)

^b UNIVERSITY OF SHIZUOKA   (Japan)

Author keywords

Aggregate; Capillary electrophoresis; Fibril; High performance liquid chromatography; Insulin; Pheochromocytoma cell

Indexed keywords

INSULIN; LACTATE DEHYDROGENASE;

ANIMAL CELL; ARTICLE; CAPILLARY ELECTROPHORESIS; CELL STRAIN; CONFORMATIONAL TRANSITION; CYTOTOXICITY; DRUG CONFORMATION; ENZYME ACTIVITY; NONHUMAN; QUALITY CONTROL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

RATTUS;

EID: 70350230379     PISSN: 13449702     EISSN: 13475207     Source Type: Journal    
DOI: 10.1248/jhs.55.825     Document Type: Article

References (15)

1. Ahmad, A., Uversky, V. N., Hong, D. and Fink, A. L. (2005) Early events in the fibrillation of monomeric insulin. J. Biol. Chem., 280, 42669-42675. (Pubitemid 43049225)
2. Jiménez, J. L., Nettleton, E. J., Bouchard, M., Robinson, C. V., Dobson, C. M. and Saibil, H. R. (2002) The protofilament structure of insulin amyloid fibrils. Proc. Natl. Acad. Sci. U.S.A., 99, 9196-9201. (Pubitemid 34764590)
3. Harrison, R. S., Sharpe, P. C., Singh, Y. and Fairlie, D. P. (2007) Amyloid peptides and proteins in review. Rev. Physiol. Biochem. Pharmacol., 159, 1-77.
4. Grudzielanek, S., Velkova, A., Shukla, A., Smirnovas, V., Tatarek-Nossol, M., Rehage, H., Kapurniotu, A. and Winter, R. (2007) Cytotoxicity of insulin within its self-assembly and amyloidogenic pathways. J. Mol. Biol., 370, 372-384. (Pubitemid 46829214)
5. Jansen, R., Grudzielanek, S., Dzwolak, W. and Winter, R. (2004) High pressure promotes circularly shaped insulin amyloid. J. Mol. Biol., 338, 203-206. (Pubitemid 38447079)
6. Grudzielanek, S., Jansen, R. and Winter, R. (2005) Solvational tuning of the unfolding, aggregation and amyloidogenesis of insulin. J. Mol. Biol., 351, 879-894. (Pubitemid 41111903)
7. Onoue, S., Ohshima, K., Debari, K., Koh, K., Shioda, S., Iwasa, S., Kashimoto, K. and Yajima, T. (2004) Mishandling of the therapeutic peptide glucagon generates cytotoxic amyloidogenic fibrils. Pharm. Res., 21, 1274-1283. (Pubitemid 38954214)
8. Onoue, S., Iwasa, S., Kojima, T., Katoh, F., Debari, K., Koh, K., Matsuda, Y. and Yajima, T. (2006) Structural transition of glucagon in the concentrated solution observed by electrophoretic and spectroscopic techniques. J. Chromatogr. A, 1109, 167-173. (Pubitemid 43358038)
9. Naiki, H., Higuchi, K., Hosokawa, M. and Takeda, T. (1989) Fluorometric-determination of amyloid fibrils in vitro using the fluorescent dye, thioflavine T. Anal. Biochem., 177, 244-249. (Pubitemid 19088253)
10. Liu, X. D., Kaczmarski, K., Cavazzini, A., Szabelski, P., Zhou, D. M. and Guiochon, G. (2002) Modeling of preparative reversed-phase HPLC of insulin. Biotechnol. Prog., 18, 796-806. (Pubitemid 34853391)
11. 25-35- induced oxidative stress and the ensuing apoptosis. J. Neurosci. Res., 81, 572-580.
12. Stephenson, R. C. and Clarke, S. (1989) Succinimide formation from aspartyl and asparaginyl peptides as a model for the spontaneous degradation of proteins. J. Biol. Chem., 264, 6164-6170.
13. Shimizu, T., Watanabe, A., Ogawara, M., Mori, H. and Shirasawa, T. (2000) Isoaspartate formation and neurodegeneration in Alzheimer's disease. Arch. Biochem. Biophys., 381, 225-234. (Pubitemid 32260308)
14. Nakamura, T., Sakai, M., Sadakane, Y., Haga, T., Goto, Y., Kinouchi, T., Saito, T. and Fujii, N. (2008) Differential rate constants of racemization of aspartyl and asparaginyl residues in human alpha Acrystallin mutants. Biochim. Biophys. Acta, 1784, 1192-1199.
15. Sadakane, Y., Yamazaki, T., Nakagomi, K., Akizawa, T., Fujii, N., Tanimura, T., Kaneda, M. and Hatanaka, Y. (2003) Quantification of the isomerization of Asp residue in recombinant human alpha A-crystallin by reversed-phase HPLC. J. Pharm. Biomed. Anal., 30, 1825-1833. (Pubitemid 35453986)