Activin/nodal inhibition alone accelerates highly efficient neural conversion from human embryonic stem cells and imposes a caudal positional identity

PLoS ONE

Volumn 4, Issue 10, 2009, Pages

  Patani, Rickie ^a   Compston, Alastair ^a   Puddifoot, Clare A ^b   Wyllie, David J A ^b   Hardingham, Giles E ^b   Allen, Nicholas D ^c   Chandran, Siddharthan ^a,b  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

^c CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

4 [4 (1,3 BENZODIOXOL 5 YL) 5 (2 PYRIDINYL) 1H IMIDAZOL 2 YL]BENZAMIDE; ACTIVIN; CALCIUM ION; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR MUSASHI; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR OTX; TRANSCRIPTION FACTOR OTX1; TRANSCRIPTION FACTOR OTX2; TRANSCRIPTION FACTOR PAX6; TRANSCRIPTION FACTOR PAX7; TRANSCRIPTION FACTOR SOX1; UNCLASSIFIED DRUG; 1,3 DIOXOLANE DERIVATIVE; 4 (5 BENZO(1,3)DIOXOL 5 YL 4 PYRIDIN 2 YL 1H IMIDAZOL 2 YL)BENZAMIDE; 4-(5-BENZO(1,3)DIOXOL-5-YL-4-PYRIDIN-2-YL-1H-IMIDAZOL-2-YL)BENZAMIDE; BENZAMIDE DERIVATIVE; PROTEIN NODAL;

ARTICLE; CALCIUM CELL LEVEL; CELL CULTURE; CELL POPULATION; CELL TRANSFORMATION; CONTROLLED STUDY; DOWN REGULATION; EMBRYONIC STEM CELL; ENZYME INHIBITION; EPIDERMIS CELL; HUMAN; HUMAN CELL; MESODERM; NERVE CELL; UPREGULATION; CELL ADHESION; CELL DIFFERENTIATION; CELL LINE; CYTOLOGY; IMMUNOHISTOCHEMISTRY; METABOLISM; MORPHOGENESIS; PATCH CLAMP; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; STEM CELL;

ACTIVINS; BENZAMIDES; BODY PATTERNING; CELL ADHESION; CELL DIFFERENTIATION; CELL LINE; DIOXOLES; EMBRYONIC STEM CELLS; HUMANS; IMMUNOHISTOCHEMISTRY; NEURONS; NODAL PROTEIN; PATCH-CLAMP TECHNIQUES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; STEM CELLS;

EID: 70350191753     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0007327     Document Type: Article

References (26)

1. Joannides AJ, Fiore-Heriche C, Battersby AA, Athauda-Arachchi P, Bouhon IA, et al. (2007) A scaleable and defined system for generating neural stem cells from human embryonic stem cells. Stem Cells 25: 731-737.
2. Chambers SM, Fasano CA, Papapetrou EP, Tomishima M, Sadelain M, et al. (2009) Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol.
3. Zhang SC, Wernig M, Duncan ID, Brustle O, Thomson JA (2001) In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat Biotechnol 19: 1129-1133.
4. Itsykson P, Ilouz N, Turetsky T, Goldstein RS, Pera MF, et al. (2005) Derivation of neural precursors from human embryonic stem cells in the presence of noggin. Mol Cell Neurosci 30: 24-36.
5. Erceg S, Lainez S, Ronaghi M, Stojkovic P, Perez-Arago MA, et al. (2008) Differentiation of human embryonic stem cells to regional specific neural precursors in chemically defined medium conditions. PLoS ONE 3: e2122.
6. Vallier L, Pedersen R (2008) Differentiation of human embryonic stem cells in adherent and in chemically defined culture conditions. Curr Protoc Stem Cell Biol Chapter 1: Unit 1D 4 1-1D 4 7.
7. Pebay A, Wong RC, Pitson SM, Wolvetang EJ, Peh GS, et al. (2005) Essential roles of sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells. Stem Cells 23: 1541-1548.
8. Ying QL, Smith AG (2003) Defined conditions for neural commitment and differentiation. Methods Enzymol 365: 327-341.
9. Bouhon IA, Kato H, Chandran S, Allen ND (2005) Neural differentiation of mouse embryonic stem cells in chemically defined medium. Brain Res Bull 68: 62-75.
10. Watanabe K, Kamiya D, Nishiyama A, Katayama T, Nozaki S, et al. (2005) Directed differentiation of telencephalic precursors from embryonic stem cells. Nat Neurosci 8: 288-296.
11. Ikeda H, Osakada F, Watanabe K, Mizuseki K, Haraguchi T, et al. (2005) Generation of Rx+/Pax6+ neural retinal precursors from embryonic stem cells. Proc Natl Acad Sci U S A 102: 11331-11336.
12. Vallier L, Reynolds D, Pedersen RA (2004) Nodal inhibits differentiation of human embryonic stem cells along the neuroectodermal default pathway. Dev Biol 275: 403-421.
13. Vallier L, Alexander M, Pedersen RA (2005) Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells. J Cell Sci 118: 4495-4509.
14. Camus A, Perea-Gomez A, Moreau A, Collignon J (2006) Absence of Nodal signaling promotes precocious neural differentiation in the mouse embryo. Dev Biol 295: 743-755.
15. Smith JR, Vallier L, Lupo G, Alexander M, Harris WA, et al. (2008) Inhibition of Activin/Nodal signaling promotes specification of human embryonic stem cells into neuroectoderm. Dev Biol 313: 107-117.
16. Stern CD (2001) Initial patterning of the central nervous system: how many organizers? Nat Rev Neurosci 2: 92-98.
17. Varlet I, Collignon J, Robertson EJ (1997) nodal expression in the primitive endoderm is required for specification of the anterior axis during mouse gastrulation. Development 124: 1033-1044.
18. Thomas P, Beddington R (1996) Anterior primitive endoderm may be responsible for patterning the anterior neural plate in the mouse embryo. Curr Biol 6: 1487-1496.
19. Matsuo I, Kuratani S, Kimura C, Takeda N, Aizawa S (1995) Mouse Otx2 functions in the formation and patterning of rostral head. Genes Dev 9: 2646-2658.
20. Gajovic S, St-Onge L, Yokota Y, Gruss P (1997) Retinoic acid mediates Pax6 expression during in vitro differentiation of embryonic stem cells. Differentiation 62: 187-192.
21. Di Giorgio FP, Boulting GL, Bobrowicz S, Eggan KC (2008) Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation. Cell Stem Cell 3: 637-648.
22. Harrington AE, Morris-Triggs SA, Ruotolo BT, Robinson CV, Ohnuma S, et al. (2006) Structural basis for the inhibition of activin signalling by follistatin. EMBO J 25: 1035-1045.
23. Baxter AW, Wyllie DJ (2006) Phosphatidylinositol 3 kinase activation and AMPA receptor subunit trafficking underlie the potentiation of miniature EPSC amplitudes triggered by the activation of L-type calcium channels. J Neurosci 26: 5456-5469.
24. Soriano FX, Martel MA, Papadia S, Vaslin A, Baxter P, et al. (2008) Specific targeting of pro-death NMDA receptor signals with differing reliance on the NR2B PDZ ligand. J Neurosci 28: 10696-10710.
25. Hardingham GE, Chawla S, Johnson CM, Bading H (1997) Distinct functions of nuclear and cytoplasmic calcium in the control of gene expression. Nature 385: 260-265.
26. Minta A, Kao JP, Tsien RY (1989) Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem 264: 8171-8178.