Generation of functional ventricular heart muscle from mouse ventricular progenitor cells

Science

Volumn 326, Issue 5951, 2009, Pages 426-429

  Dornian, Ibrahim J ^a,b,f   Chiravuri, Murali ^a   Meer, Van Der Peter ^a,c   Feinberg, Adam W ^d   Shi, Xi ^a   Shao, Ying ^a   Wu, Sean M ^a,b   Parker, Kevin Kit ^b,d,e   Chien, Kenneth R ^a,b,f  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD STEM CELL INSTITUTE   (United States)

^c UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

^d HARVARD UNIVERSITY   (United States)

^e HARVARD UNIVERSITY   (United States)

^f Harvard University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RED FLUORESCENT PROTEIN;

DIFFERENTIATION; GENETIC ENGINEERING; GENOME; MAMMAL; MOLECULAR ANALYSIS; RODENT;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CELL EXPANSION; CELL LINEAGE; CONTROLLED STUDY; EMBRYO; EMBRYO DEVELOPMENT; EMBRYONIC STEM CELL; GENETIC TRANSCRIPTION; HEART MUSCLE CELL; HEART VENTRICLE WALL; MOUSE; MUSCLE FUNCTION; NONHUMAN; PRIORITY JOURNAL; STEM CELL;

ACTION POTENTIALS; ANIMALS; CELL CYCLE; CELL DIFFERENTIATION; CELL LINE; CELL LINEAGE; CELLS, CULTURED; EMBRYONIC STEM CELLS; GENE EXPRESSION; HEART; HEART VENTRICLES; MICE; MICE, TRANSGENIC; MUSCLE DEVELOPMENT; MYOCARDIAL CONTRACTION; MYOCYTES, CARDIAC; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; TISSUE ENGINEERING; VENTRICULAR FUNCTION;

MAMMALIA;

EID: 70350131483     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1177350     Document Type: Article

References (23)

1. M. Buckingham, S. Meilhac, S. Zaffran, Nat. Rev. Genet. 6, 826 (2005).
2. S. Martin-Puig, Z. Wang, K. R. Chien, Cell Stem Cell 2, 320 (2008).
3. E. Dodou, S. M. Xu, B. L. Black, Mech. Dev. 120, 1021 (2003).
4. Y. Oyang et al., Cell Stem Cell 1, 165 (2007).
5. Materials and methods are available as supporting material on Science Online.
6. C. L. Lien et al., Development 126, 75 (1999).
7. S. M. Wu et al., Cell 127, 1137 (2006).
8. E. Dodou, M. P. Verzi, J. P. Anderson, S. M. Xu, B. L. Black, Development 131, 3931 (2004).
9. M. P. Verzi, D. J. McCulley, S. De Val, E. Dodou, B. L. Black, Dev. Biol. 287, 134 (2005).
10. M. Reich et al, Nat. Genet. 38, 500 (2006).
11. B. G. Bruneau et al., Cell 106, 709 (2001).
12. A. D. Mori et al., Dev. Biol. 297, 566 (2006).
13. A. Barrallo-Gimeno, M. A. Nieto, Development 132, 3151 (2005).
14. M. J. Blanco et al, Development 134, 4073 (2007).
15. A. W. Feinberg et al., Science 317, 1366 (2007).
16. K. L. Laugwitz et al., Nature 433, 647 (2005).
17. A. Moretti et al., Cell 127, 1151 (2006).
18. C. L. Cai et al., Dev. Cell 5, 877 (2003).
19. L. Bu et al., Nature 460, 113 (2009).
20. K. Takahashi et al., Cell 131, 861 (2007).
21. J. Yu et al., Science 318, 1917 (2007); published online 19 November 2007 (10.1126/science.1151526).
22. J. Hanna et al., Cell 133, 250 (2008).
23. We thank L. Prickett and K. Folz-Donahue for expert help with flow cytometry. Financial support was provided by the de Gunzburg Family Foundation (to I.J.D.), the NIH fellowship support (T32 HL002807 to I.J.D. and M.C.), the Netherlands Organization for Scientific Research (Rubicon 825-07-011 to P.v.d.M.), and the Leducq Foundation (to K.R.C.). The authors have filed patent applications pertaining to the isolation and expansion of isl cells and the development and use of muscular thin film from CVPs as described herein. Microarray data has been uploaded to the National Center for Biotechnology Information-Gene Expression Omnibus database with accession number GSE17513.