A functional comparison of the TET aminopeptidases of P. furiosus and B. subtilis with a protein-engineered variant recombining the former's structure with the latter's active site

Enzyme and Microbial Technology

Volumn 46, Issue 1, 2010, Pages 1-8

  Kapoor, Divya ^a   Singh, Balvinder ^a   Karthikeyan, Subramanian ^a   Guptasarma, Purnananda ^a  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

Author keywords

Active site transplantation; Enzyme characterization; Enzyme engineering; Protein engineering

Indexed keywords

ACTIVE SITE; ACTIVE SITE TRANSPLANTATION; AMINO PEPTIDASE; BACILLUS SUBTILIS; ENZYME CHARACTERIZATION; ENZYME ENGINEERING; GUIDED MUTATION; OPTIMAL FUNCTION; PROTEIN ENGINEERING; PYROCOCCUS FURIOSUS; SUBTILIS;

BACTERIOLOGY; BIOCHEMICAL ENGINEERING; GENETIC ENGINEERING;

ENZYMES;

AMINOPEPTIDASE; TETRAHEDRAL AMINOPEPTIDASE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL MUTATION; BACTERIUM MUTANT; CIRCULAR DICHROISM; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; GEL FILTRATION CHROMATOGRAPHY; LIGHT SCATTERING; MOLECULAR CLONING; NONHUMAN; PH; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PYROCOCCUS FURIOSUS; TEMPERATURE;

BACILLUS SUBTILIS; PYROCOCCUS FURIOSUS;

EID: 70350127725     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2009.09.003     Document Type: Article

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