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Volumn 184, Issue 2, 2009, Pages 294-302

Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: Differential expression of neuronal and glial protein markers

Author keywords

Adult neuron culture; Amyloid precursor protein; Glia; Neuron; Transfection

Indexed keywords

CELL MARKER; GLIAL FIBRILLARY ACIDIC PROTEIN; GREEN FLUORESCENT PROTEIN; LUCIFERASE; NEURON SPECIFIC ENOLASE; TAU PROTEIN;

EID: 70350038033     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2009.08.018     Document Type: Article
Times cited : (23)

References (34)
  • 2
    • 34447526608 scopus 로고    scopus 로고
    • Neuronal differentiation is accompanied by increased levels of SNAP-25 protein in fetal rat primary cortical neurons: implications in neuronal plasticity and Alzheimer's disease
    • Bailey J.A., and Lahiri D.K. Neuronal differentiation is accompanied by increased levels of SNAP-25 protein in fetal rat primary cortical neurons: implications in neuronal plasticity and Alzheimer's disease. Ann NY Acad Sci 1086 (2006) 54-65
    • (2006) Ann NY Acad Sci , vol.1086 , pp. 54-65
    • Bailey, J.A.1    Lahiri, D.K.2
  • 3
    • 0019216477 scopus 로고
    • Trophic interactions between astroglial cells and hippocampal neurons in culture
    • Banker G.A. Trophic interactions between astroglial cells and hippocampal neurons in culture. Science 209 (1980) 809-810
    • (1980) Science , vol.209 , pp. 809-810
    • Banker, G.A.1
  • 4
    • 0017350927 scopus 로고
    • Rat hippocampal neurons in dispersed cell culture
    • Banker G.A., and Cowan W.M. Rat hippocampal neurons in dispersed cell culture. Brain Res 126 (1977) 397-442
    • (1977) Brain Res , vol.126 , pp. 397-442
    • Banker, G.A.1    Cowan, W.M.2
  • 6
    • 0029954687 scopus 로고    scopus 로고
    • A new syntaxin family member implicated in targeting of intracellular transport vesicles
    • Bock J.B., Lin R.C., and Scheller R.H. A new syntaxin family member implicated in targeting of intracellular transport vesicles. J Biol Chem 271 (1996) 17961-17965
    • (1996) J Biol Chem , vol.271 , pp. 17961-17965
    • Bock, J.B.1    Lin, R.C.2    Scheller, R.H.3
  • 7
    • 39749173021 scopus 로고    scopus 로고
    • Added astroglia promote greater synapse density and higher activity in neuronal networks
    • Boehler M.D., Wheeler B.C., and Brewer G.J. Added astroglia promote greater synapse density and higher activity in neuronal networks. Neuron Glia Biol 3 (2007) 127-140
    • (2007) Neuron Glia Biol , vol.3 , pp. 127-140
    • Boehler, M.D.1    Wheeler, B.C.2    Brewer, G.J.3
  • 8
    • 0017184389 scopus 로고
    • A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    • Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 (1976) 248-254
    • (1976) Anal Biochem , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 9
    • 0030896414 scopus 로고    scopus 로고
    • Isolation and culture of adult rat hippocampal neurons
    • Brewer G.J. Isolation and culture of adult rat hippocampal neurons. J Neurosci Methods 71 (1997) 143-155
    • (1997) J Neurosci Methods , vol.71 , pp. 143-155
    • Brewer, G.J.1
  • 10
    • 34250718676 scopus 로고    scopus 로고
    • Isolation and culture of adult neurons and neurospheres
    • Brewer G.J., and Torricelli J.R. Isolation and culture of adult neurons and neurospheres. Nat Protoc 2 (2007) 1490-1498
    • (2007) Nat Protoc , vol.2 , pp. 1490-1498
    • Brewer, G.J.1    Torricelli, J.R.2
  • 11
    • 0027226219 scopus 로고
    • Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination
    • Brewer G.J., Torricelli J.R., Evege E.K., and Price P.J. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J Neurosci Res 35 (1993) 567-576
    • (1993) J Neurosci Res , vol.35 , pp. 567-576
    • Brewer, G.J.1    Torricelli, J.R.2    Evege, E.K.3    Price, P.J.4
  • 12
    • 0033561111 scopus 로고    scopus 로고
    • Markers of nerve tissue injury in the cerebrospinal fluid in patients with lumbar disc herniation and sciatica
    • Brisby H., Olmarker K., Rosengren L., Cederlund C.G., and Rydevik B. Markers of nerve tissue injury in the cerebrospinal fluid in patients with lumbar disc herniation and sciatica. Spine 24 (1999) 742-746
    • (1999) Spine , vol.24 , pp. 742-746
    • Brisby, H.1    Olmarker, K.2    Rosengren, L.3    Cederlund, C.G.4    Rydevik, B.5
  • 13
    • 33644770187 scopus 로고    scopus 로고
    • Structure and function of SNARE and SNARE-interacting proteins
    • Brunger A.T. Structure and function of SNARE and SNARE-interacting proteins. Q Rev Biophys 38 (2005) 1-47
    • (2005) Q Rev Biophys , vol.38 , pp. 1-47
    • Brunger, A.T.1
  • 14
    • 0027499070 scopus 로고
    • The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity
    • Crouch S.P., Kozlowski R., Slater K.J., and Fletcher J. The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. J Immunol Methods 160 (1993) 81-88
    • (1993) J Immunol Methods , vol.160 , pp. 81-88
    • Crouch, S.P.1    Kozlowski, R.2    Slater, K.J.3    Fletcher, J.4
  • 15
    • 70350031954 scopus 로고    scopus 로고
    • Efficient non-viral transfection of primary human adult chondrocytes in a high-throughput format
    • Haag J., Voigt R., Soeder S., and Aigner T. Efficient non-viral transfection of primary human adult chondrocytes in a high-throughput format. Osteoarthritis Cartilage (2008)
    • (2008) Osteoarthritis Cartilage
    • Haag, J.1    Voigt, R.2    Soeder, S.3    Aigner, T.4
  • 16
    • 58149512072 scopus 로고    scopus 로고
    • In-tube transfection improves the efficiency of gene transfer in primary neuronal cultures
    • Halterman M.W., Giuliano R., Dejesus C., and Schor N.F. In-tube transfection improves the efficiency of gene transfer in primary neuronal cultures. J Neurosci Methods 177 (2009) 348-354
    • (2009) J Neurosci Methods , vol.177 , pp. 348-354
    • Halterman, M.W.1    Giuliano, R.2    Dejesus, C.3    Schor, N.F.4
  • 17
    • 34548864370 scopus 로고    scopus 로고
    • Culturing hippocampal neurons
    • Kaech S., and Banker G. Culturing hippocampal neurons. Nat Protoc 1 (2006) 2406-2415
    • (2006) Nat Protoc , vol.1 , pp. 2406-2415
    • Kaech, S.1    Banker, G.2
  • 19
    • 0025967258 scopus 로고
    • The promoter activity of the gene encoding Alzheimer beta-amyloid precursor protein (APP) is regulated by two blocks of upstream sequences
    • Lahiri D.K., and Robakis N.K. The promoter activity of the gene encoding Alzheimer beta-amyloid precursor protein (APP) is regulated by two blocks of upstream sequences. Brain Res Mol Brain Res 9 (1991) 253-257
    • (1991) Brain Res Mol Brain Res , vol.9 , pp. 253-257
    • Lahiri, D.K.1    Robakis, N.K.2
  • 20
    • 0026704256 scopus 로고
    • Expression of tau protein in non-neuronal cells: microtubule binding and stabilization
    • Lee G., and Rook S.L. Expression of tau protein in non-neuronal cells: microtubule binding and stabilization. J Cell Sci 102 Pt 2 (1992) 227-237
    • (1992) J Cell Sci , vol.102 , Issue.PART 2 , pp. 227-237
    • Lee, G.1    Rook, S.L.2
  • 21
    • 0036127407 scopus 로고    scopus 로고
    • Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death
    • Lesuisse C., and Martin L.J. Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death. J Neurobiol 51 (2002) 9-23
    • (2002) J Neurobiol , vol.51 , pp. 9-23
    • Lesuisse, C.1    Martin, L.J.2
  • 22
    • 0032514633 scopus 로고    scopus 로고
    • Memory-enhancing effects of secreted forms of the beta-amyloid precursor protein in normal and amnestic mice
    • Meziane H., Dodart J.C., Mathis C., Little S., Clemens J., Paul S.M., and Ungerer A. Memory-enhancing effects of secreted forms of the beta-amyloid precursor protein in normal and amnestic mice. Proc Natl Acad Sci USA 95 (1998) 12683-12688
    • (1998) Proc Natl Acad Sci USA , vol.95 , pp. 12683-12688
    • Meziane, H.1    Dodart, J.C.2    Mathis, C.3    Little, S.4    Clemens, J.5    Paul, S.M.6    Ungerer, A.7
  • 23
    • 39549093321 scopus 로고    scopus 로고
    • Neurodegeneration and neuroinflammation in cdk5/p25-inducible mice: a model for hippocampal sclerosis and neocortical degeneration
    • Muyllaert D., Terwel D., Kremer A., Sennvik K., Borghgraef P., Devijver H., Dewachter I., and Van Leuven F. Neurodegeneration and neuroinflammation in cdk5/p25-inducible mice: a model for hippocampal sclerosis and neocortical degeneration. Am J Pathol 172 (2008) 470-485
    • (2008) Am J Pathol , vol.172 , pp. 470-485
    • Muyllaert, D.1    Terwel, D.2    Kremer, A.3    Sennvik, K.4    Borghgraef, P.5    Devijver, H.6    Dewachter, I.7    Van Leuven, F.8
  • 24
    • 0022850973 scopus 로고
    • Microtubule-associated proteins
    • Olmsted J.B. Microtubule-associated proteins. Annu Rev Cell Biol 2 (1986) 421-457
    • (1986) Annu Rev Cell Biol , vol.2 , pp. 421-457
    • Olmsted, J.B.1
  • 25
    • 0027538182 scopus 로고
    • Proliferation, differentiation, and long-term culture of primary hippocampal neurons
    • Ray J., Peterson D.A., Schinstine M., and Gage F.H. Proliferation, differentiation, and long-term culture of primary hippocampal neurons. Proc Natl Acad Sci USA 90 (1993) 3602-3606
    • (1993) Proc Natl Acad Sci USA , vol.90 , pp. 3602-3606
    • Ray, J.1    Peterson, D.A.2    Schinstine, M.3    Gage, F.H.4
  • 26
    • 0026535505 scopus 로고
    • Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system
    • Reynolds B.A., and Weiss S. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255 (1992) 1707-1710
    • (1992) Science , vol.255 , pp. 1707-1710
    • Reynolds, B.A.1    Weiss, S.2
  • 27
    • 0033019645 scopus 로고    scopus 로고
    • Ionic mechanism of isoflurane's actions on thalamocortical neurons
    • Ries C.R., and Puil E. Ionic mechanism of isoflurane's actions on thalamocortical neurons. J Neurophysiol 81 (1999) 1802-1809
    • (1999) J Neurophysiol , vol.81 , pp. 1802-1809
    • Ries, C.R.1    Puil, E.2
  • 28
    • 41149174313 scopus 로고    scopus 로고
    • Clinical anesthesia causes permanent damage to the fetal guinea pig brain
    • Rizzi S., Carter L.B., Ori C., and Jevtovic-Todorovic V. Clinical anesthesia causes permanent damage to the fetal guinea pig brain. Brain Pathol 18 (2008) 198-210
    • (2008) Brain Pathol , vol.18 , pp. 198-210
    • Rizzi, S.1    Carter, L.B.2    Ori, C.3    Jevtovic-Todorovic, V.4
  • 30
    • 6944231363 scopus 로고    scopus 로고
    • Gene structure and organization of the human beta-secretase (BACE) promoter
    • Sambamurti K., Kinsey R., Maloney B., Ge Y.W., and Lahiri D.K. Gene structure and organization of the human beta-secretase (BACE) promoter. FASEB J 18 (2004) 1034-1036
    • (2004) FASEB J , vol.18 , pp. 1034-1036
    • Sambamurti, K.1    Kinsey, R.2    Maloney, B.3    Ge, Y.W.4    Lahiri, D.K.5
  • 31
    • 70449526416 scopus 로고    scopus 로고
    • Activity-dependent survival of neurons in culture: a model of slow neurodegeneration
    • Schonfeld-Dado E., and Segal M. Activity-dependent survival of neurons in culture: a model of slow neurodegeneration. J Neural Transm (2009)
    • (2009) J Neural Transm
    • Schonfeld-Dado, E.1    Segal, M.2
  • 32
    • 0035066332 scopus 로고    scopus 로고
    • Alzheimer's disease: genes, proteins, and therapy
    • Selkoe D.J. Alzheimer's disease: genes, proteins, and therapy. Physiol Rev 81 (2001) 741-766
    • (2001) Physiol Rev , vol.81 , pp. 741-766
    • Selkoe, D.J.1
  • 33
    • 0032517141 scopus 로고    scopus 로고
    • Molecular cloning of the promoter of the gene encoding the Rhesus monkey beta-amyloid precursor protein: structural characterization and a comparative study with other species
    • Song W., and Lahiri D.K. Molecular cloning of the promoter of the gene encoding the Rhesus monkey beta-amyloid precursor protein: structural characterization and a comparative study with other species. Gene 217 (1998) 151-164
    • (1998) Gene , vol.217 , pp. 151-164
    • Song, W.1    Lahiri, D.K.2
  • 34
    • 0032032480 scopus 로고    scopus 로고
    • Astrocytes protect neurons from neurotoxic injury by serum glutamate
    • Ye Z.C., and Sontheimer H. Astrocytes protect neurons from neurotoxic injury by serum glutamate. Glia 22 (1998) 237-248
    • (1998) Glia , vol.22 , pp. 237-248
    • Ye, Z.C.1    Sontheimer, H.2


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