RecA gene sequence and Multilocus Sequence Typing for species-level resolution of Burkholderia cepacia complex isolates

Letters in Applied Microbiology

Volumn 49, Issue 5, 2009, Pages 580-588

  Cesarini, S ^a   Bevivino, A ^a   Tabacchioni, S ^a   Chiarini, L ^a   Dalmastri, C ^a  

^a ENEA C R CASACCIA   (Italy)

Author keywords

Burkholderia cepacia complex; Multilocus Sequence Typing; RecA based identification

Indexed keywords

ARBORIS; BURKHOLDERIA; BURKHOLDERIA CEPACIA COMPLEX; CYSTIC FIBROSIS; GENE SEQUENCES; MULTILOCUS SEQUENCE TYPING; RECA-BASED IDENTIFICATION;

RECA PROTEIN;

BACTERIUM; ENVIRONMENTAL MANAGEMENT; EPIDEMIOLOGY; GENE; HEALTH SERVICES; POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIUM ISOLATION; BURKHOLDERIA CEPACIA; CONTROLLED STUDY; GENE SEQUENCE; MULTILOCUS SEQUENCE TYPING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SEQUENCE ANALYSIS; SPECIES DIFFERENCE; UNINDEXED SEQUENCE;

BURKHOLDERIA; BURKHOLDERIA CENOCEPACIA; BURKHOLDERIA CEPACIA COMPLEX;

EID: 70350023185     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2009.02709.x     Document Type: Article

References (38)

1. Baldwin, A., Mahenthiralingam, E., Thickett, K.M., Honeybourne, D., Maiden, M.C., Govan, J.R.W., Speert, D.P., LiPuma, J.J. et al. (2005) Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex. J Clin Microbiol 43, 4665 4673.
2. Baldwin, A., Mahenthiralingam, E. Drevinek, P. (2007) Environmental Burkholderia cepacia complex isolates in human infections. Emerg Infect Dis 13, 458 461.
3. Bazzicalupo, M. Fani, R. (1996) The use of RAPD for generating specific DNA probes for microorganisms. In Methods in Molecular Biology Vol. 50 Species Diagnostic Protocols. PCR and other Nucleic Acid Methods ed. Clapp, J.P. pp. 155 175. Totowa, NJ : Humana Press Inc.
4. Bevivino, A., Dalmastri, C., Tabacchioni, S., Chiarini, L., Belli, M.L., Piana, S., Materazzo, A., Vandamme, P. et al. (2002) Burkholderia cepacia complex bacteria from clinical and environmental sources in Italy: genomovar status and distribution of traits related to virulence and transmissibility. J Clin Microbiol 40, 846 851.
5. Chiarini, L., Bevivino, A., Dalmastri, C., Tabacchioni, S. Visca, P. (2006) Burkholderia cepacia complex species: health hazards and biotechnological potential. Trends Microbiol 14, 277 286.
6. Coenye, T. LiPuma, J.J. (2007) Epidemiology, typing, and population genetics of Burkholderia species. In Burkholderia Molecular Microbiology and Genomics ed. Coenye, T. Vandamme, P. pp. 29 52. UK : Horizon Bioscience.
7. Coenye, T., Vandamme, P., Govan, J.R.W. LiPuma, J.J. (2001) Taxonomy and identification of the Burkholderia cepacia complex. J Clin Microbiol 39, 3427 3436.
8. Dalmastri, C., Pirone, L., Tabacchioni, S., Bevivino, A. Chiarini, L. (2005) Efficacy of species-specific recA PCR tests in the identification of Burkholderia cepacia complex environmental isolates. FEMS Microbiol Lett 246, 39 45.
9. Dalmastri, C., Baldwin, A., Tabacchioni, S., Bevivino, A., Mahenthiralingam, E., Chiarini, L. Dowson, C.G. (2007) Investigating Burkholderia cepacia complex populations recovered from Italian maize rhizosphere by multilocus sequence typing. Environ Microbiol 9, 1632 1639.
10. Fiore, A., Laevens, S., Bevivino, A., Dalmastri, C., Tabacchioni, S., Vandamme, P. Chiarini, L. (2001) Burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in Italy. Environ Microbiol 3, 137 143.
11. Govan, J.R.W. (2000) Infection control in cystic fibrosis: methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa and the Burkholderia cepacia complex. J R Soc Med 93 (Suppl. 38 40 45.
12. Govan, J.R.W. Deretic, V. (1996) Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol Rev 60, 539 574.
13. Govan, J.R., Brown, A.R. Jones, A.M. (2007) Evolving epidemiology of Pseudomonas aeruginosa and the Burkholderia cepacia complex in cystic fibrosis lung infection. Future Microbiol 2, 153 164.
14. Holmes, A., Nolan, R., Taylor, R., Finley, R., Riley, M., Jiang, R.Z., Steinbach, S. Goldstein, R. (1999) An epidemic of Burkholderia cepacia transmitted between patients with and without cystic fibrosis. J Infect Dis 179, 1197 1205.
15. Jolley, K.A., Chan, M.S. Maiden, M.C. (2004) mlstdbNet - distributed multi-locus sequence typing (MLST) databases. BMC Bioinformatics 5, 86.
16. Kim, J.Y., Sajjan, U.S., Krasan, G.P. LiPuma, J.J. (2005) Disruption of tight junctions during traversal of the respiratory epithelium by Burkholderia cenocepacia. Infect Immun 73, 7107 7112.
17. Lee, Y.A. Chan, C.W. (2007) Molecular typing and presence of genetic markers among strains of banana finger-tip rot pathogen, Burkholderia cenocepacia in Taiwan. Phytopathology 97, 195 201.
18. Lynch, K.H. Dennis, J.J. (2008) Development of a species-specific fur gene-based method for identification of the Burkholderia cepacia complex. J Clin Microbiol 46, 447 455.
19. LiPuma, J.J. (1998) Burkholderia cepacia. Management issues and new insights. Clin Chest Med 19, 473 486.
20. LiPuma, J.J., Spilker, T., Coenye, T. Gonzalez, C.F. (2002) An epidemic Burkholderia cepacia complex strain identified in soil. Lancet 359, 2002 2003.
21. Mahenthiralingam, E., Bischof, J., Byrne, S.K., Radomski, C., Davies, J.E., Av-Gay, Y. Vandamme, P. (2000) DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J Clin Microbiol 38, 3165 3173.
22. Mahenthiralingam, E., Baldwin, A. Vandamme, P. (2002) Burkholderia cepacia complex infection in patients with cystic fibrosis. J Med Microbiol 51, 533 538. (Pubitemid 34747981)
23. Mahenthiralingam, E., Urban, T.A. Goldberg, J.B. (2005) The multifarious, multireplicon Burkholderia cepacia complex. Nat Rev Microbiol 3, 144 156. (Pubitemid 40207595)
24. Mahenthiralingam, E., Baldwin, A. Dowson, C.G. (2008) Burkholderia cepacia complex bacteria: opportunistic pathogens with important natural biology. J Appl Microbiol 104, 1539 1551.
25. Manno, G., Dalmastri, C., Tabacchioni, S., Vandamme, P., Lorini, R., Minicucci, L., Romano, L., Giannattasio, A. et al. (2004) Epidemiology and clinical course of Burkholderia cepacia complex infections, particularly those caused by different Burkholderia cenocepacia strains, among patients attending an Italian cystic fibrosis center. J Clin Microbiol 42, 1491 1497.
26. Miller, S.C., LiPuma, J.J. Parke, J.L. (2002) Culture-based and non-growth-dependent detection of the Burkholderia cepacia complex in soil environments. Appl Environ Microbiol 68, 3750 3758.
27. Moore, J.E., Millar, B.C., Xu, J., Crowe, M., Redmond, A.O.B. Elborn, J.S. (2002) Misidentification of a genomovar of Burkholderia cepacia by recA restriction fragment length polymorphism. J Clin Pathol 55, 309 311.
28. Payne, G.W., Vandamme, P., Morgan, S.H., LiPuma, J.J., Coenye, T., Weightman, A.J., Jones, T.H. Mahenthiralingam, E. (2005) Development of a recA gene-based identification approach for the entire Burkholderia genus. Appl Environ Microbiol 71, 3917 3927.
29. Pirone, L., Chiarini, L., Dalmastri, C., Bevivino, A. Tabacchioni, S. (2005) Detection of cultured and uncultured Burkholderia cepacia complex bacteria naturally occurring in the maize rhizosphere. Environ Microbiol 7, 1734 1742.
30. Pirone, L., Bragonzi, A., Farcomeni, A., Paroni, M., Auriche, C., Conese, M., Chiarini, L., Dalmastri, C. et al. (2008) Burkholderia cenocepacia strains isolated from cystic fibrosis patients are apparently more invasive and more virulent than rhizosphere strains. Environ Microbiol 10, 2773 2784.
31. Vandamme, P., Holmes, B., Vancanneyet, M., Coenye, T., Hoste, B., Coopman, R., Reverts, H., Lauwers, S. et al. (1997) Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. Int J Syst Bacteriol 47, 1188 1200.
32. Vandamme, P., Henry, D., Coenye, T., Nuzla, S., Vancanneyt, M., LiPuma, J.J., Speert, D.P., Govan, J.R.W. et al. (2002) Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools. FEMS Immunol Med Microbiol 33, 143 149.
33. Vanlaere, E. (2008) Burkholderia cepacia complex: new developments in taxonomy, diagnosis and epidemiology. PhD thesis, Faculty of Sciences, Ghent University, Ghent, Belgium.
34. Vanlaere, E., LiPuma, J.J., Baldwin, A., Henry, D., De Brandt, E., Mahenthiralingam, E., Speert, D., Dowson, C.G. et al. (2008) Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. Int J Syst Evol Microbiol 58, 1580 1590.
35. Vanlaere, E., Baldwin, A., Gevers, D., Henry, D., De Brandt, E., LiPuma, J.J., Mahenthiralingam, E., Speert, D.P. et al. (2009) Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. Int J Syst Evol Microbiol 59, 102 111.
36. Vermis, K., Vandekerckhove, C., Nelis, H.J. Vandamme, P. (2002) Evaluation of restriction fragment length polymorphism analysis of 16S rDNA as a tool for genomovar characterisation within the Burkholderia cepacia complex. FEMS Microbiol Lett 214, 1 5.
37. Vermis, K., Brachkova, M., Vandamme, P. Nelis, H. (2003) Isolation of Burkholderia cepacia complex genomovars from waters. Syst Appl Microbiol 26, 595 600. (Pubitemid 37486518)
38. Vermis, K., Coenye, T., LiPuma, J.J., Mahenthiralingam, E., Nelis, H.J. Vandamme, P. (2004) Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov. Int J Syst Evol Microbiol 54, 689 691.