Quantification of three key enzymes involved in artemisinin biogenesis in Artemisia annua by polyclonal antisera-based ELISA

Plant Molecular Biology Reporter

Volumn 27, Issue 1, 2009, Pages 50-57

  Zeng, Qing Ping ^a   Zeng, Xiao Mei ^a   Yin, Lu Lu ^a   Yang, Rui Yi ^a   Feng, Li Ling ^a   Yang, Xue Qin ^a  

^a GUANGZHOU UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

Artemisinin biosynthesis; Gene expression; Immunoquantification; Translational regulation

Indexed keywords

AMORPHA; ARTEMISIA ANNUA;

EID: 70350000874     PISSN: 07359640     EISSN: 15729818     Source Type: Journal    
DOI: 10.1007/s11105-008-0056-1     Document Type: Article

References (33)

1. Bharel S, Gulati A, Abdin MZ, et al. Enzymatic synthesis of artemisinin from natural and synthetic precursors. J Nat Prod 1998;61:633-6. doi:10.1021/np970024s.
2. Bouwmeester HJ, Wallaart TE, Janssen MH, et al. Amorpha-4, 11-diene synthase catalyze the first probable step in artemisinin biosynthesis. Phytochemistry 1999;52:843-54. doi:10.1016/S0031-9422(99)00206-X.
3. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;7:248-54. doi:10.1016/0003-2697(76)90527-3.
4. Brown GD, Sy LK. In vivo transformations of artemisinic acid in Artemisia annua plants. Tetrahedron 2007;63:9548-66. doi:10.1016/j.tet.2007.06.062.
5. Cane DE. Biosynthesis of sesquiterpene. In: Porter JW, Spurgeon SL, editors. Biosynthesis of isoprenoid compounds, vols. 1 and 2. New York: Wiley; 1981. p. 283-374.
6. Covello PS, Teoh KH, Polichuk DR, et al. Functional genomics and the biosynthesis of artemisinin. Phytochemistry 2007;68:1864-71. doi:10.1016/j.phytochem.2007.02.016.
7. Dhingra V, Narasu ML. Purification and characterization of an enzyme involved in biochemical transformation of arteannuin B to artemisinin from Artemisia annua. Biochem Biophys Res Commun 2001;281:558-61. doi:10.1006/bbrc.2000.4197.
8. Dhingra V, Rajoli C, Narasu ML. Partial purification of proteins involved in the bioconversion of arteannuin B to artemisinin. Bioresour Technol 2000;73:279-82. doi:10.1016/S0960-8524(99)00152-2.
9. Ferreira JFS, Janick J. Immunoquantitative analysis of artemisinin from Artemisia annua using polyclonal antibodies. Phytochemistry 1996;41:97-104. doi:10.1016/0031-9422(95)00542-0.
10. Ferreira JFS, Simon JE, Janick J. Artemisia annua: botany, horticulture and pharmacology. Hortic Rev (Am Soc Hortic Sci) 1997;19:319-71.
11. Gechev T, Willekens H, van Montagu M, et al. Different responses of tobacco antioxidant enzymes to light and chilling stress. J Plant Physiol 2003;160:509-15. doi:10.1078/0176-1617-00753.
12. Hsu E. The history of qing hao in Chinese material medica. Trans R Soc Trop Med Hyg 2006;100:505-8. doi:10.1016/j.trstmh.2005.09.020.
13. Huang Y, Yin LL, Feng LL, et al. Cloning of amorpha-4,11-diene synthase gene from Artemisia annua and expression in Escherichia coli. J Guangzhou U Chin Med 2008;25:68-73.
14. Kindermans JM, Pilloy J, Olliaro P, et al. Ensuring sustained ACT production and reliable artemisinin supply. Malar J 2007;6:125-30. doi:10.1186/1475-2875-6-125.
15. Laughlin JC. Post-harvest drying treatment effects on antimalarial constituents of Artemisia annua L. Acta Hortic 2002;576:315-20.
16. Mercke P, Bengtsson M, Bouwmeester HJ, et al. Molecular cloning, expression, and characterization of amorpha-4, 11-diene synthase, a key enzyme of artemisinin biosynthesis in Artemisia annua L. Arch Biochem Biophys 2000;381:173-80. doi:10.1006/abbi.2000.1962.
17. Mishra NP, Mishra RK, Singhai GS. Changes in the activities of anti-oxidant enzymes during exposure of intact wheat leaves to strong visible light at different temperatures in the presence of protein synthesis inhibitors. Plant Physiol 1993;102:903-10.
18. Nair MSR, Basile DV. Bioconversion of arteannuin B to artemisinin. J Nat Prod 1993;56:1559-66. doi:10.1021/np50099a015.
19. O'Kane D, Gill V, Boyd P, et al. Chilling, oxidative stress and antioxidant responses in Arabidopsis thaliana callus. Planta 1996;198:371-7. doi:10.1007/BF00620053.
20. Ro DK, Paradise EM, Ouellet M, et al. Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature 2006;440:940-3. doi:10.1038/nature04640.
21. Sangwan RS, Agarwal K, Luthra R, et al. Biotransformation of arteannuic acid into arteannuin B and artemisinin in Artemisia annua. Phytochemistry 1993;34:1301-2. doi:10.1016/0031-9422(91)80019-W.
22. Teoh KH, Polichuk DR, Reed DW, et al. Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin. FEBS Lett 2006;580:1411-6. doi:10.1016/j.febslet.2006.01.065.
23. Wallaart TE, van Uden W, Lubberink HG, et al. Isolation and identification of dihydroartemisinic acid from Artemisia annua and its possible role in the biosynthesis of artemisinin. J Nat Prod 1999;62:430-3. doi:10.1021/np980370p.
24. Wallaart TE, Pras N, Beekman AC, et al. Seasonal variation of artemisinin and its biosynthetic precursors in plants of Artemisia annua of different geographical origin: proof for the existence of chemotypes. Planta Med 2000;66:57-62. doi:10.1055/s-2000-11115.
25. Wallaart TE, Bouwmeester HJ, Hille J, et al. Amorpha-4,11-diene synthase: cloning and functional expression of a key enzyme in the biosynthetic pathway of the novel antimalarial drug artemisinin. Planta 2001;212:460-5. doi:10.1007/s004250000428.
26. Wang Y, Xia ZQ, Zhou FY, et al. Studies on biosynthesis of artemisinin: the key intermediate-artemisinic acid in biosynthesis of artemisinin and arteannuin B. Acta Chimi Sin 1988;46:1152-3.
27. Xu XL, Yang RY, Yang XQ, et al. A quantitative assay of recombinant malarial lactate dehydrogenase as a platform for screening inhibitors from crude herbal extracts. Chin J Biotechnol 2007;23:593-7. doi:10.1016/S1872-2075(07)60038-7.
28. Yang RY, Zeng QP, Feng LL, et al. Quantitative transcript profiling reveals down-regulation of a sterol pathway relevant gene and overexpression of artemisinin biogenetic genes in transgenic Artemisia annua plants. Planta Med. 2008; in press.
29. Yin LL, Zhao C, Huang Y, et al. Abiotic stress-induced expression of artemisinin biosynthesis genes in Artemisia annua L. Chin App Environ Biol 2008;14:1-5.
30. Zeng QP, Qiu F, Yuan L. Production of artemisinin by genetically modified microbes. Biotechnol Lett 2008a;30:581-92. doi:10.1007/s10529-007-9596-y.
31. Zeng QP, Zhao C, Yin LL, et al. Artemisinin biosynthetic cDNA and novel EST cloning and quantification on low temperature-induced expression. Sci China Ser C 2008b;51:232-44. doi:10.1007/s11427-008-0032-x.
32. Zeng QP, Yang RY, Feng LL, et al. Genetic and environmental regulation and artificial metabolic manipulation for artemisinin biosynthesis. In: Richter FW, editor. Biotechnology: Research, Technology and Applications. New York: Nova Science; in press, 2008c.
33. Zhang YS, Teoh KH, Reed DW, et al. The molecular cloning of artemisinic aldehyde Δ11(13) reductase and its role in glandular trichome-dependent biosynthesis of artemisinin in Artemisia annua. J Biol Chem 2008;283:21501-8.