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Volumn 1216, Issue 45, 2009, Pages 7851-7864
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Engineering of a two-step purification strategy for a panel of monoclonal immunoglobulin M directed against undifferentiated human embryonic stem cells
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Author keywords
Adsorption isotherm; Adsorption kinetics; Anion exchange chromatography; Benzonase; IgM; Number of binding sites; PEG; Precipitation
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Indexed keywords
ADSORPTION KINETICS;
ANION-EXCHANGE CHROMATOGRAPHY;
BENZONASE;
IGM;
NUMBER OF BINDING SITES;
PEG;
PRECIPITATION;
ADSORPTION;
ADSORPTION ISOTHERMS;
BINDING ENERGY;
BINDING SITES;
CELL CULTURE;
CHROMATOGRAPHIC ANALYSIS;
CHROMATOGRAPHY;
IONS;
MONOCLONAL ANTIBODIES;
NUCLEIC ACIDS;
POLYETHYLENE OXIDES;
PURIFICATION;
RESINS;
POLYETHYLENE GLYCOLS;
DNA;
ENDONUCLEASE;
IMMUNOGLOBULIN M;
MACROGOL;
MONOCLONAL ANTIBODY;
POLYMER;
RESIN;
ANION EXCHANGE CHROMATOGRAPHY;
ARTICLE;
BINDING KINETICS;
CELL DIFFERENTIATION;
CONCENTRATION (PARAMETERS);
CONTROLLED STUDY;
DNA DEGRADATION;
EMBRYO;
EMBRYONIC STEM CELL;
ENZYME LINKED IMMUNOSORBENT ASSAY;
FLOW CYTOMETRY;
GEL PERMEATION CHROMATOGRAPHY;
HUMAN;
HUMAN CELL;
HYBRIDOMA CELL CULTURE;
ISOELECTRIC FOCUSING;
PH;
POLYACRYLAMIDE GEL ELECTROPHORESIS;
POROSITY;
PRECIPITATION;
PRIORITY JOURNAL;
PROCESS OPTIMIZATION;
PROTEIN PURIFICATION;
SUPERNATANT;
ANIMALS;
ANTIBODIES, MONOCLONAL;
CELL DIFFERENTIATION;
CHROMATOGRAPHY, ION EXCHANGE;
CYTOTOXICITY TESTS, IMMUNOLOGIC;
EMBRYONIC STEM CELLS;
FRACTIONAL PRECIPITATION;
HUMANS;
HYBRIDOMAS;
IMMUNOGLOBULIN M;
MICE;
POLYETHYLENE GLYCOLS;
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EID: 70349925848
PISSN: 00219673
EISSN: None
Source Type: Journal
DOI: 10.1016/j.chroma.2009.09.059 Document Type: Article |
Times cited : (22)
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References (49)
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