Improved gap-repair cloning method that uses oligonucleotides to target cognate sequences

Yeast

Volumn 26, Issue 9, 2009, Pages 497-505

  Kitazono, Ana A ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

Gap filling cloning; In vivo cloning; Plasmid construction; pRS plasmids

Indexed keywords

OLIGONUCLEOTIDE; PLASMID DNA; PROTEIN CAK1; PROTEIN CDC28; PROTEIN CIN5; PROTEIN CLB2; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM COLONY; CONTROLLED STUDY; DNA FRAGMENTATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENOMIC FRAGMENT; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RESTRICTION SITE; SEQUENCE ANALYSIS; AMINO ACID SEQUENCE; EVALUATION; GENE VECTOR; GENETICS; METHODOLOGY; MOLECULAR GENETICS; YEAST;

ESCHERICHIA COLI;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; PLASMIDS; YEASTS;

EID: 70349914012     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1680     Document Type: Article

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