Biochemical and functional characterization of a metalloprotease from the thermophilic fungus thermoascus aurantiacus

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 19, 2009, Pages 9210-9217

  Merheb Dini, Carolina ^a   Cabral, Hamilton ^b   Leite, Rodrigo S R ^a   Zanphorlin, Letícia M ^a   Okamoto, Débora N ^a   Rodriguez, Gustavo O Bonilla ^a   Juliano, Luiz ^c   Arantes, Eliane C ^b   Gomes, Eleni ^a   Da Silva, Roberto ^a  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Fluorescent peptides; Metalloprotease; N terminal sequence; Solid state fermentation; Thermoascus aurantiacus; Thermostability

Indexed keywords

CASEIN; METALLOPROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HEAT; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; THERMOASCUS;

AMINO ACID SEQUENCE; CASEINS; ENZYME STABILITY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HOT TEMPERATURE; KINETICS; METALLOPROTEASES; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; SUBSTRATE SPECIFICITY; THERMOASCUS;

FUNGI; THERMOASCUS; THERMOASCUS AURANTIACUS;

EID: 70349902496     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf9017977     Document Type: Article

References (40)

1. Andrade, V. S.; Sarubbo, L. A.; Fukushima, K.; Miyaji, M.; Nishimura, K.; Campos-Takaki, G. M. Production of extracellular protease by Mucor Circinelloides using D-glucose as carbon source/ substrate. Braz. J. Microbiol. 2002, 33, 106-110.
2. Gomes, E.; Guez, M. A. U.; Martin, N.; Da-Silva, R. Enzimas temioestáveis: fontes, produção e aplicação industrial. Quim. Nova 2001, 30, 136-145.
3. (3) Merheb, C. W.; Cabral, H.; Gomes, E.; Da-Silva, R. Partial characterization of protease from a thermophilic fungus, Thermoascus aurantiacus, and its hydrolytic activity on bovine casein. Food Chem. 2007, 104, 127-131. (Pubitemid 46562547)
4. Hadj-Ali, N.; Agrebi, R.; Ghorbel-Frikha, B.; Sellami-Kamoun, A.; Kanoun, S.; Nasri, M. Biochemical and molecular characterization of a detergent stable alkaline serine-protease from a newly isolated Bacillus lkheniformis NH1. Enzyme Microb. Technol, 2007, 40, 515-523.
5. Johnvesly, B.; Manjunath, B. R.; Naik, G. R. Pigeon pea waste as a novel, inexpensive, substrate for production of a thermostable alkaline protease from thermoalkalophilic Bacillus sp. JB-99. Bioresour. Technol. 2002, 82, 61-64.
6. Li, D. C.; Yang, Y. J.; Shen, C. Y. Protease production by the thermophilic fungus Thermomyces lanuginosus. Mycol. Res. 1997, 101, 18-22.
7. Hashimoto, H.; Iwaasa, T.; Yokotsuka, T. Some properties of acid protease from the thermophilic fungus, Penicillium duponti K1014. Appl. Microbiol. 1973, 25, 578-583.
8. Ong, P. S.; Gaucher, G. M. Production, purification and characterization of thermomycolase, the extracellular serine protease of the thermophilic fungus Malbranchea pulchella var. sulfurea. Can. J. Microbiol. 1916, 22, 165-176.
9. Sumantha, A.; et al. Production and partial purification of a neutral metaloprotease by fungal mixed substrate fermentation. Food Technol. Biotechnol. 2005, 43, 313-319.
10. Kembhavi, A. A.; Kulkarni, A.; Pant, A. Salt-tolerant and thermostable alkaline protease from Bacillus subtilis NCIM N° 64. Appl. Biochem. Biotechnol. 1993, 38, 83-92.
11. (11) Germano, S.; Pandey, A.; Osaka, C. A.; Rocha, S. N.; Soccol, C. R. Characterization and stability of protease from Penicillium sp. produced by solid-state fermentation. Enzyme Microb. Technol. 2003, 32, 246-251. (Pubitemid 36050900)
12. Hartree, E. F. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal. Biochem. 1912, 48, 422-427.
13. Laemmli, U. K. Cleavage of structural protein during the assembly of head of bacteriophage T4. Nature 1970, 227, 680-685.
14. Sambrook, J.; Russell, D. W. Molecular Cloning: A laboratory manual, 3; Cold Spring Harbor Laboratory Press: Woodbury, NY, 2001.
15. Blum, H.; Bier, H.; Gross, H. J. Improved silver staining of plantproteins, RNA and DNA in Polyacrylamide gels. Eletrophoresis. 1987, 8, 93-99.
16. Leatherbarrow, R. J. Grafit Version 5.0; Erithacus Software Ltd.: Staines, U.K., 1992.
17. Hirata, Y.; Cezari, M. H. S.; Nakaie, C., R.; Boschcov, P.; Ito, A. S.; Juliano, A. M.; Juliano, L. Internally quenched fluorogenic protease substrates: Solid-phase synthesis and fluorescence spectroscopy of peptides containing ortho-aminobenzoyl/dinitrophenyl groups as donor-acceptor pairs. Lett. Peptide Sci. 1994, J, 299-308.
18. Schecheter, I.; Berger, A. On the size of the active site in proteases. I. Papain. Biochem. Biophys. Res. Commun. 1967, 27, 157-162.
19. (19) Klemencic, I.; Carmona, A. K.; Cezari, M. H. S.; Juliano, M. A.; Juliano, L.; Guncar, G.; Turk, D.; Krizaj, I.; Turk, V.; Turk, B. Biochemical characterization of human cathepsin X revealed that the enzyme is an exopeptidase, acting as carboxymonopeptidase or carboxydipeptidase. Eur. J. Biochem. 2000, 267, 5404-5412. (Pubitemid 30679256)
20. (20) Angelo, P. F.; Lima, A. R.; Alves, F. M.; Blaber, S. I.; Scarisbrick, I. A.; Blaber, M.; Juliano, L.; Juliano, M. A. Substrate specificity of human kallikrein 6: salt and glycosaminoglycan activation effects. J. Biol. Chem. 2006, 281, 3116-3126. (Pubitemid 43845921)
21. Chakrabarti, S. K.; Matsumura, N.; Rami, R. S. Purification and characterization of an extracellular alkaline serine protease from Aspergillus terreus (IJIRA 6.2). Curr. Microbiol. 2000, 40, 239-244.
22. Tunga, R.; Shrivastava, B.; Banerjee, R. Purification and characterization of a protease from solid state cultures of Aspergillus parasitueus. Process Biochem. 2003, 38, 1553-1558.
23. Barata, R. A.; Andrade, M. H. G.; Rodrigues, R. D.; Castro, I. M. Purification and characterization of an extracellular trypsin-like protease of Fusarium oxysporum var. lini. J. Biosci. Bioeng. 2002, 94, 304-308.
24. Hatanaka, T.; Uesugi, J. A. Y.; Iwabuchi, M. Purification, characterization cloning, and sequencing of metalloendopeptidase from Streptomyces septatus TH-2. Arch. Biochem. Biophys. 2005, 434, 289-298.
25. Ko, H.-S.; Yokoyama, Y.; Ohno, N.; Okadome, M.; Amachi, S.; Shinoyama, H.; Fujii, T. Purification and characterization of intracellular and extracellular, thermostable and alkali-tolerant alcohol oxidases produced by a thermophilic fungus, Thermoascus aurantiacus NBRC 31693. J. Biosci. Bioeng. 2005, 99, 348-353.
26. Parry, N. J.; Beever, D. E.; Owen, E.; Nerinckx, W.; Claeyssens, M.; Van Beeumen, J.; Bhat, M. K. Biochemical characterization and mode of action of a thermostable endoglucanase purified from Thermoascus aurantiacus. Arch. Biochem. Biophys. 2002, 404, 243-253.
27. (27) Da-Silva, R.; Lago, W. S.; Merheb, C. W.; Macchione, M. M.; Park, Y. K.; Gomes, E. Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacus miehe. Braz. J. Microbiol. 2005, 36, 235-241. (Pubitemid 43297988)
28. Sookkheo, B.; Sinchaikul, S.; Phutrakul, S.; Chen, S.-T. Purification and characterization of the highly thermostable protease from Bacillus stearothermophilus TLS33. Protein Expression Purif 2000, 20, 142-151.
29. Nascimento, W. C. A.; Martins, M. L. L. Production and properties of an extracellular protease from thermophilic Bacillus sp. Braz. J. Microbiol 2004, 35, 91-96.
30. Rocco, A. G.; Mollica, L.; Ricchiuto, P.; Baptista, A. M.; Gianazza, E.; Eberini, I. Characterization of the protein unfolding processes induced by urea and temperature. Biophys. J. 2008, 94, 2241-2251.
31. Vieille, C.; Zeikus, G. J. Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability. Microbiol. Mol. Biol. Rev. 2001, 65, 1-43.
32. Wang, S.-L.; Yang, C.-H.; Liang, T.-W.; Yen, Y.-H. Optimization of conditions for protease production by Chryseobacterium taeanense TKU001. Bioresour. Technol. 2008, 99, 3700-3707.
33. Li, J.; Yang, J.; Huang, X.; Zhang, K.-Q. Purification and characterization of an extracellular serine protease from Clonostachys rosea and its potential as a pathogenic factor. Process Biochem. 2006, 41, 925-929.
34. Romero, F. J.; Garcia, L. A.; Salas, J. A.; Diaz, M.; Quirós, L. M. Production, purification and partial characterization of two extracellular proteases from Serratia mareescens grown in whey. Process Biochem. 2001, 36, 507-515.
35. (35) Setyorini, E.; Takenaka, S.; Murakami, S.; Aoki, K. Purification and characterization of two novel halotolerant extracellular proteases from Bacillus subtilis strain FP-133. Biosci. Biotechnol. Biochem. 2006, 70, 433-440. (Pubitemid 43289425)
36. (36) Zhao, H. Effect of ions and other compatible solutes on enzyme activity, and its implication for biocatalysis using ionic liquids. J. Mol. Catal. B: Enzym. 2005, 37, 12-25. (Pubitemid 41674643)
37. Rao, M. B.; Tanksale, A. M.; Ghatge, M. S.; Deshpande, V. V. Molecular and Biotechnological Aspects of Microbial Proteases. Microbiol. Mol. Biol. Rev. 1998, 3, 597-635.
38. (38) Bersanetti, P. A.; Park, H.-Y.; Bae, K. S.; Son, K.-H.; Shin, D.-H.; Hirata, I. Y.; Juliano, M. A.; Carmona, A. K.; Juliano, L. Characterization of arazyme, an exocellular metalloprotease isolated from Serratia proteamaculans culture medium. Enzyme Microb. Technol. 2005, 37, 574-581. (Pubitemid 41306244)
39. (39) Altschul, S. F.; Madden, T. L.; Schäffer, A. A.; Zhang, J.; Zhang, Z.; Miller, W.; Lipman, D. J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25, 3389-3402. (Pubitemid 27359211)
40. Loftus, B. J.; et al. The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans. Science 2005, 307, 1321-1324.