An SOS Inhibitor that Binds to Free RecA Protein: The PsiB Protein

Molecular Cell

Volumn 36, Issue 1, 2009, Pages 121-130

  Petrova, Vessela ^a   Chitteni Pattu, Sindhu ^a   Drees, Julia C ^a   Inman, Ross B ^a   Cox, Michael M ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

DNA

Indexed keywords

BACTERIAL PROTEIN; LEXA PROTEIN; NUCLEOPROTEIN; PSIB PROTEIN; RAD51 PROTEIN; RECA PROTEIN; RECOMBINASE; SINGLE STRANDED DNA; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIUM CONJUGATION; CONTROLLED STUDY; DNA BINDING; DNA STRAND; ENZYME REGULATION; NONHUMAN; PLASMID; PROTEIN BINDING; PROTEIN CLEAVAGE;

ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEINS; CONJUGATION, GENETIC; CROSSING OVER, GENETIC; DNA; DNA, CIRCULAR; DNA, SINGLE-STRANDED; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLUORESCENCE POLARIZATION; MODELS, GENETIC; POLY T; PROTEIN BINDING; REC A RECOMBINASES; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES; SOS RESPONSE (GENETICS);

BACTERIA (MICROORGANISMS);

EID: 70349775652     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcel.2009.07.026     Document Type: Article

References (49)

1. Aguero M.E., Aron L., DeLuca A.G., Timmis K.N., and Cabello F.C. A plasmid-encoded outer membrane protein, TraT, enhances resistance of Escherichia coli to phagocytosis. Infect. Immun. 46 (1984) 740-746
2. Anderson D.G., and Kowalczykowski S.C. The translocating RecBCD enzyme stimulates recombination by directing RecA protein onto ssDNA in a chi-regulated manner. Cell 90 (1997) 77-86
3. Bagdasarian M., Bailone A., Angulo J.F., Scholz P., and Devoret R. PsiB, an anti-SOS protein, is transiently expressed by the F sex factor during its transmission to an Escherichia coli K-12 recipient. Mol. Microbiol. 6 (1992) 885-893
4. Bagdasarian M., Bailone A., Bagdasarian M.M., Manning P.A., Lurz R., Timmis K.N., and Devoret R. An inhibitor of SOS induction, specified by a plasmid locus in Escherichia coli. Proc. Natl. Acad. Sci. USA 83 (1986) 5723-5726
5. Bagdasarian M., D'Ari R., Filipowicz W., and George J. Suppression of induction of SOS functions in an Escherichia coli tif-1 mutant by plasmid R100.1. J. Bacteriol. 141 (1980) 464-469
6. Bailone A., Backman A., Sommer S., Celerier J., Bagdasarian M.M., Bagdasarian M., and Devoret R. PsiB polypeptide prevents activation of RecA protein in Escherichia coli. Mol. Gen. Genet. 214 (1988) 389-395
7. Busygina V., Sehorn M.G., Shi I.Y., Tsubouchi H., Roeder G.S., and Sung P. Hed1 regulates Rad51-mediated recombination via a novel mechanism. Genes Dev. 22 (2008) 786-795
8. Centore R.C., Lestini R., and Sandler S.J. XthA (Exonuclease III) regulates loading of RecA onto DNA substrates in log phase Escherichia coli cells. Mol. Microbiol. 67 (2008) 88-101
9. Cox M.M. The bacterial RecA protein as a motor protein. Annu. Rev. Microbiol. 57 (2003) 551-577
10. Drees J.C., Chitteni-Pattu S., McCaslin D.R., Inman R.B., and Cox M.M. Inhibition of RecA protein function by the RdgC protein from Escherichia coli. J. Biol. Chem. 281 (2006) 4708-4717
11. Drees J.C., Lusetti S.L., Chitteni-Pattu S., Inman R.B., and Cox M.M. A RecA filament capping mechanism for RecX protein. Mol. Cell 15 (2004) 789-798
12. Dutreix M., Backman A., Celerier J., Bagdasarian M.M., Sommer S., Bailone A., Devoret R., and Bagdasarian M. Identification of psiB genes of plasmids F and R6-5. Molecular basis for psiB enhanced expression in plasmid R6-5. Nucleic Acids Res. 16 (1988) 10669-10679
13. Edelhoch H. Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry 6 (1967) 1948-1954
14. Eggler A.L., Lusetti S.L., and Cox M.M. The C terminus of the Escherichia coli RecA protein modulates the DNA binding competition with single-stranded DNA-binding protein. J. Biol. Chem. 278 (2003) 16389-16396
15. Frost L.S., Ippen-Ihler K., and Skurray R.A. Analysis of the sequence and gene products of the transfer region of the F sex factor. Microbiol. Rev. 58 (1994) 162-210
16. Grassucci R.A., Taylor D.J., and Frank J. Preparation of macromolecular complexes for cryo-electron microscopy. Nat. Protoc. 2 (2007) 3239-3246
17. Gruenig M.C., Renzette N., Long E., Chitteni-Pattu S., Inman R.B., Cox M.M., and Sandler S.J. RecA-mediated SOS induction requires an extended filament conformation but no ATP hydrolysis. Mol. Microbiol. 69 (2008) 1165-1179
18. Kanamoto T., Hellman U., Heldin C.H., and Souchelnytskyi S. Functional proteomics of transforming growth factor-beta1-stimulated Mv1Lu epithelial cells: Rad51 as a target of TGFbeta1-dependent regulation of DNA repair. EMBO J. 21 (2002) 1219-1230
19. Kowalczykowski S.C., and Krupp R.A. Effects of Escherichia coli SSB protein on the single-stranded DNA-dependent ATPase activity of Escherichia coli RecA protein: evidence that SSB protein facilitates the binding of RecA protein to regions of secondary structure within single-stranded DNA. J. Mol. Biol. 193 (1987) 97-113
20. Krejci L., Damborsky J., Thomsen B., Duno M., and Bendixen C. Molecular dissection of interactions between Rad51 and members of the recombination-repair group. Mol. Cell. Biol. 21 (2001) 966-976
21. Lavery P.E., and Kowalczykowski S.C. Properties of recA441 protein-catalyzed DNA strand exchange can be attributed to an enhanced ability to compete with SSB protein. J. Biol. Chem. 265 (1990) 4004-4010
22. Lindsley J.E., and Cox M.M. Dissociation pathway for RecA nucleoprotein filaments formed on linear duplex DNA. J. Mol. Biol. 205 (1989) 695-711
23. Lindsley J.E., and Cox M.M. Assembly and disassembly of RecA protein filaments occurs at opposite filament ends: relationship to DNA strand exchange. J. Biol. Chem. 265 (1990) 9043-9054
24. Little J.W. Mechanism of specific LexA cleavage-autodigestion and the role of RecA coprotease. Biochimie 73 (1991) 411-422
25. Little J.W., Edmiston S.H., Pacelli L.Z., and Mount D.W. Cleavage of the Escherichia coli LexA protein by the RecA protease. Proc. Natl. Acad. Sci. USA 77 (1980) 3225-3229
26. Lohman T.M., and Overman L.B. Two binding modes in Escherichia coli single strand binding protein-single stranded DNA complexes: modulation by NaCl concentration. J. Biol. Chem. 260 (1985) 3594-3603
27. Lopper M., Boonsombat R., Sandier S.J., and Keck J.L. A hand-off mechanism for primosome assembly in replication restart. Mol. Cell 26 (2007) 781-793
28. Lujan S.A., Guogas L.M., Ragonese H., Matson S.W., and Redinbo M.R. Disrupting antibiotic resistance propagation by inhibiting the conjugative DNA relaxase. Proc. Natl. Acad. Sci. USA 104 (2007) 12282-12287
29. Lusetti S.L., Drees J.C., Stohl E.A., Seifert H.S., and Cox M.M. The DinI and RecX proteins are competing modulators of RecA function. J. Biol. Chem. 279 (2004) 55073-55079
30. Lusetti S.L., Voloshin O.N., Inman R.B., Camerini-Otero R.D., and Cox M.M. The DinI protein stabilizes RecA protein filaments. J. Biol. Chem. 279 (2004) 30037-30046
31. Lusetti S.L., Wood E.A., Fleming C.D., Modica M.J., Korth J., Abbott L., Dwyer D.W., Roca A.I., Inman R.B., and Cox M.M. C-terminal deletions of the Escherichia coli RecA protein-characterization of in vivo and in vitro effects. J. Biol. Chem. 278 (2003) 16372-16380
32. Marcandier S., Granger-Schnarr M., and Cozzone A.J. Phosphorylation of Escherichia coli proteins during the SOS response. Int. J. Biochem. 26 (1994) 387-396
33. Marmorstein L.Y., Ouchi T., and Aaronson S.A. The BRCA2 gene product functionally interacts with p53and RAD51. Proc. Natl. Acad. Sci. USA 95 (1998) 13869-13874
34. Masai H., and Arai K. Frpo: a novel single-stranded DNA promoter for transcription and for primer RNA synthesis of DNA replication. Cell 89 (1997) 897-907
35. Messing J. New M13 vectors for cloning. Methods Enzymol. 101 (1983) 20-78
36. Morimatsu K., and Kowalczykowski S.C. RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair. Mol. Cell 11 (2003) 1337-1347
37. Morrical S.W., Lee J., and Cox M.M. Continuous association of Escherichia coli single-stranded DNA binding protein with stable complexes of RecA protein and single-stranded DNA. Biochemistry 25 (1986) 1482-1494
38. Neuendorf S.K., and Cox M.M. Exchange of RecA protein between adjacent RecA protein-single-stranded DNA complexes. J. Biol. Chem. 261 (1986) 8276-8282
39. Petrushenko Z.M., Budkevich T.V., Shalak V.F., Negrutskii B.S., and El'skaya A.V. Novel complexes of mammalian translation elongation factor eEF1A.GDP with uncharged tRNA and aminoacyl-tRNA synthetase: implications for tRNA channeling. Eur. J. Biochem. 269 (2002) 4811-4818
40. Roca A.I., and Cox M.M. RecA protein: structure, function, and role in recombinational DNA repair. Prog. Nucleic Acid Res. Mol. Biol. 56 (1997) 129-223
41. Sakai A., and Cox M.M. RecFOR and RecOR as distinct RecA loading pathways. J. Biol. Chem. 284 (2009) 3264-3272
42. Slilaty S.N., and Little J.W. Lysine-156 and serine-119 are required for LexA repressor cleavage: a possible mechanism. Proc. Natl. Acad. Sci. USA 84 (1987) 3987-3991
43. Sommer S., Knezevic J., Bailone A., and Devoret R. Induction of only one SOS operon, umuDC, is required for SOS mutagenesis in Escherichia coli. Mol. Gen. Genet. 239 (1993) 137-144
44. Stohl E.A., Brockman J.P., Burkle K.L., Morimatsu K., Kowalczykowski S.C., and Siefert H.S. Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo. J. Biol. Chem. 278 (2003) 2278-2285
45. Tanaka K., Hiramoto T., Fukuda T., and Miyagawa K. A novel human rad54 homologue, Rad54B, associates with Rad51. J. Biol. Chem. 275 (2000) 26316-26321
46. Veaute X., Delmas P., Selva M., Jeusset J., Le Cam E., Matic I., Fabre F., and Petit M.A. UvrD helicase, unlike Rep helicase, dismantles RecA nucleoprotein filaments in Escherichia coli. EMBO J. 24 (2005) 180-189
47. Walker G.C., Smith B.T., and Sutton M.D. The SOS response to DNA damage. In: Storz G., and Hengge-Aronis R. (Eds). Bacterial Stress Responses (2000), American Society of Microbiology, Washington, D.C. 131-144
48. Wolkow C.A., DeBoy R.T., and Craig N.L. Conjugating plasmids are preferred targets for Tn7. Genes Dev. 10 (1996) 2145-2157
49. Yuan Z.M., Huang Y., Ishiko T., Nakada S., Utsugisawa T., Kharbanda S., Wang R., Sung P., Shinohara A., Weichselbaum R., and Kufe D. Regulation of Rad51 function by c-Abl in response to DNA damage. J. Biol. Chem. 273 (1998) 3799-3802