Cloning, sequence analysis, and expression of gene alyPI encoding an alginate lyase from marine bacterium Pseudoalteromonas sp. CY24

Canadian Journal of Microbiology

Volumn 55, Issue 9, 2009, Pages 1113-1118

  Duan, Gaofei ^a   Han, Feng ^a   Yu, Wengong ^a  

^a OCEAN UNIVERSITY OF CHINA   (China)

Author keywords

Alginate lyase; Cloning; Expression; Pseudoalteromonas

Indexed keywords

ALGINATE LYASE; AMINO ACID RESIDUES; ELECTROPHORETIC HOMOGENEITY; ENCODING GENES; EXPRESSION; HIS-TAG; MARINE BACTERIUM; OPEN READING FRAME; PCR TECHNIQUES; PSEUDOALTEROMONAS; SEQUENCE ANALYSIS; SIGNAL PEPTIDE; SODIUM ALGINATES;

AFFINITY CHROMATOGRAPHY; AMINATION; AMINES; AMINO ACIDS; CALCIUM; CHROMATOGRAPHIC ANALYSIS; CLONING; ENCODING (SYMBOLS); ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENE ENCODING; MANGANESE; MANGANESE COMPOUNDS; ORGANIC ACIDS; SODIUM; SODIUM CHLORIDE;

ALGINATE;

ALGINATE LYASE; ALGINIC ACID; AMINO ACID; BACTERIAL PROTEIN; NICKEL; POLYHISTIDINE TAG; SEPHAROSE; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

AMINO ACID; BACTERIUM; BIOASSAY; CATION; CLONE; ELECTROKINESIS; ENZYME ACTIVITY; GENE EXPRESSION; HOMOGENEITY; PEPTIDE; PH; POLYMERASE CHAIN REACTION; SODIUM CHLORIDE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL GENE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DEGRADATION; ENZYME ACTIVITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE EXPRESSION; GENE SEQUENCE; HOMOZYGOSITY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOALTEROMONAS;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA, BACTERIAL; ENZYME ACTIVATORS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; POLYSACCHARIDE-LYASES; PROTEIN SORTING SIGNALS; PSEUDOALTEROMONAS; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOALTEROMONAS; PSEUDOALTEROMONAS SP.;

EID: 70349766916     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W09-051     Document Type: Article

References (19)

1. Baron, A.J., Wong, T.Y., Hicks, S.J., Gacesa, P., Willcock, D., and McPherson, M.J. 1994. Alginate lyase from Klebsiella pneumoniae, subsp. aerogenes: gene cloning, sequence analysis and high-level production in Escherichia coli. Gene, 143(1): 61-66. doi:10.1016/0378-1119(94)90605-X. PMID:8200539.
2. Boyd, A., Ghosh, M., May, T.B., Shinabarger, D., Keogh, R., and Chakrabarty, A.M. 1993. Sequence of the algL gene of Pseudomonas aeruginosa and purification of its alginate lyase product. Gene, 131(1): 1-8. doi:10.1016/0378-1119(93)90662-M. PMID: 8370530.
3. Chavagnat, F., Duez, C., Guinand, M., Potin, P., Barbeyron, T., Henrissat, B., et al. 1996. Cloning, sequencing and overexpression in Escherichia coli of the alginatelyase-encoding aly gene of Pseudomonas alginovora: identification of three classes of alginate lyases. Biochem. J. 319(Pt. 2): 575-583. PMID:8912697.
4. Gacesa, P. 1988. Alginates. Carbohydr. Polym. 8(3): 161-182. doi:10.1016/0144-8617(88)90001-X.
5. Gacesa, P. 1992. Enzymic degradation of alginates. Int. J. Biochem. 24(4): 545-552. doi:10.1016/0020-711X(92)90325-U. PMID:1516726.
6. Govan, J.R., Fyfe, J.A., and Jarman, T.R. 1981. Isolation of alginate-producing mutants of Pseudomonas fluorescens, Pseudomonas putida and Pseudomonas mendocina. J. Gen. Microbiol. 125(1): 217-220. PMID:6801192.
7. Han, F., Gong, Q.H., Song, K., Li, J.B., and Yu, W.G. 2004. Cloning, sequence analysis and expression of gene alyVI encoding alginate lyase from marine bacterium Vibrio sp. QY101. DNA Seq. 15(5-6): 344-350. doi:10.1080/10425170400019300. PMID:15621659.
8. Kawamoto, H., Horibe, A., Miki, Y., Kimura, T., Tanaka, K., Nakagawa, T., et al. 2006. Cloning and sequencing analysis of alginate lyase genes from the marine bacterium Vibrio sp. O2. Mar. Biotechnol. (NY), 8(5): 481-490. doi:10.1007/s10126-005-6157-z. PMID:16810458.
9. Kim, D.E., Lee, E.Y., and Kim, H.S. 2009. Cloning and characterization of alginate lyase from a marine bacterium Streptomyces sp. ALG-5. Mar. Biotechnol. (NY), 11(1): 10-16. doi:10.1007/s10126-008-9114-9. PMID:18553118.
10. Kobayashi, T., Uchimura, K., Miyazaki, M., Nogi, Y., and Horikoshi, K. 2009. A new high-alkaline alginate lyase from a deepsea bacterium Agarivorans sp. Extremophiles, 13(1): 121-129. doi:10.1007/s00792-008-0201-7. PMID:19002649.
11. Ma, C., Lu, X., Shi, C., Li, J., Gu, Y., Ma, Y., et al. 2007. Molecular cloning and characterization of a novel beta-agarase, AgaB, from marine Pseudoalteromonas sp. CY24. J. Biol. Chem. 282(6): 3747-3754. doi:10.1074/jbc.M607888200. PMID:17166842.
12. Maki, H., Mori, A., Fujiyama, K., Kinoshita, S., and Yoshida, T. 1993. Cloning, sequence analysis and expression in Escherichia coli of a gene encoding an alginate lyase from Pseudomonas sp. OS-ALG-9. J. Gen. Microbiol. 139(5): 987-993. PMID:8336113.
13. Malissard, M., Duez, C., Guinand, M., Vacheron, M.J., Michel, G., Marty, N., et al. 1993. Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate. FEMS Microbiol. Lett. 110(1): 101-106. doi:10.1111/j.1574-6968.1993.tb06302.x. PMID:8319887.
14. Matsubara, Y., Kawada, R., Iwasaki, K., Kimura, Y., Oda, T., and Muramatsu, T. 2000. Cloning and sequence analysis of a gene (aly PG) encoding poly(alpha-L-guluronate)lyase from Corynebacterium sp. strain ALY-1. J. Biosci. Bioeng. 89(2): 199-202. doi:10.1016/S1389-1723(00)88738-1. PMID:16232727.
15. Sawabe, T., Takahashi, H., Ezura, Y., and Gacesa, P. 2001. Cloning, sequence analysis and expression of Pseudoalteromonas elyakovii IAM 14594 gene (alyPEEC) encoding the extracellular alginate lyase. Carbohydr. Res. 335(1): 11-21. doi:10.1016/S0008-6215(01)00198-7. PMID:11553350.
16. Sugimura, I.I., I, Sawabe, T., and Ezura, Y. 2000. Cloning and sequence analysis of Vibrio halioticoli genes encoding three types of polyguluronate lyase. Mar. Biotechnol. (NY), 2(1): 65-73. doi:10.1007/s101269900010. PMID:10804241.
17. Wong, T.Y., Preston, L.A., and Schiller, N.L. 2000. ALGINATE LYASE: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and applications. Annu. Rev. Microbiol. 54(1): 289-340. doi:10.1146/annurev.micro.54.1.289. PMID:11018131.
18. Yamasaki, M., Ogura, K., Hashimoto, W., Mikami, B., and Murata, K. 2005. A structural basis for depolymerization of alginate by polysaccharide lyase family-7. J. Mol. Biol. 352(1): 11-21. doi:10.1016/j.jmb.2005.06.075. PMID:16081095.
19. Yoon, H.J., Hashimoto, W., Miyake, O., Okamoto, M., Mikami, B., and Murata, K. 2000. Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp. A1 alginate lyases. Protein Expr. Purif. 19(1): 84-90. doi:10.1006/prep.2000.1226. PMID:10833394.