Heterophilic antibodies may be a cause of falsely low total IGF1 levels

European Journal of Endocrinology

Volumn 161, Issue 4, 2009, Pages 561-565

  Brugts, M P ^a   Luermans, J G L M ^b   Lentjes, E G W M ^c   Van Trooyen Van Vrouwerff, N J ^b   Van Der Horst, F A L ^b   Slee, P H Th J ^b   Lamberts, S W J ^a   Janssen, J A M L ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

^b ST ANTONIUS HOSPITAL   (Netherlands)

^c UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

GROWTH HORMONE RELEASING FACTOR; HETEROPHILE ANTIBODY; HETEROPHILIC BLOCKING TUBE; REAGENT; SOMATOMEDIN C; TESTOSTERONE; THYROTROPIN; THYROXINE; UNCLASSIFIED DRUG; AUTOANTIBODY; HORMONE; HUMAN GROWTH HORMONE; SOMATOMEDIN C RECEPTOR;

ADULT; ARTICLE; CASE REPORT; CLINICAL FEATURE; DIAGNOSTIC ERROR; FALSE POSITIVE RESULT; FREE THYROXINE INDEX; GROWTH HORMONE BLOOD LEVEL; GROWTH HORMONE DEFICIENCY; HORMONAL REGULATION; HUMAN; HYPOGONADISM; HYPOTHYROIDISM; MALE; MEASUREMENT ERROR; MEMORY DISORDER; OSTEOPENIA; PHYSICAL PERFORMANCE; PRIORITY JOURNAL; TESTOSTERONE BLOOD LEVEL; WEIGHT REDUCTION; BLOOD; ENZYME ACTIVATION; LABORATORY DIAGNOSIS; METABOLISM; MIDDLE AGED; PHYSIOLOGY;

AUTOANTIBODIES; ENZYME ACTIVATION; FALSE POSITIVE REACTIONS; HORMONES; HUMAN GROWTH HORMONE; HUMANS; INSULIN-LIKE GROWTH FACTOR I; MALE; MIDDLE AGED; RECEPTOR, IGF TYPE 1;

EID: 70349760824     PISSN: 08044643     EISSN: 1479683X     Source Type: Journal    
DOI: 10.1530/EJE-09-0316     Document Type: Article

References (29)

1. Furlanetto RW, Underwood LE, Van Wyk JJ & D'Ercole AJ. Estimation of somatomedin-C levels in normals and patients with pituitary disease by radioimmunoassay. Journal of Clinical Investigation 1977 60 648-657.
2. Clemmons DR. Commercial assays available for insulin-like growth factor I and their use in diagnosing growth hormone deficiency. Hormone Research 2001 55 73-79. (Pubitemid 33757957)
3. Rajaram S, Baylink DJ & Mohan S. Insulin-like growth factor-binding proteins in serum and other biological fluids: regulation and functions. Endocrine Reviews 1997 18 801-831. (Pubitemid 28009568)
4. Chestnut RE & Quarmby V. Evaluation of total IGF-I assay methods using samples from type I and type II diabetic patients. Journal of Immunological Methods 2002 259 11-24. (Pubitemid 33124380)
5. Clemmons DR. Quantitative measurement of IGF-I and its use in diagnosing and monitoring treatment of disorders of growth hormone secretion. Endocrine Development 2005 9 55-65.
6. Baum HB, Biller BM, Katznelson L, Oppenheim DS, Clemmons DR, Cannistraro KB, Schoenfeld DA, Best SA & Klibanski A. Assessment of growth hormone (GH) secretion in men with adult-onset GH deficiency compared with that in normal men - a Clinical Research Center Study. Journal of Clinical Endocrinology and Metabolism 1996 81 84-92. (Pubitemid 26035008)
7. Hoffman DM, Nguyen TV, O'Sullivan AJ, Baxter RC & Ho KK. Diagnosis of growth hormone deficiency in adults. Lancet 1994 344 482-483. (Pubitemid 24248279)
8. Barkan AL, Beitins IZ & Kelch RP. Plasma insulin-like growth factor-I/somatomedin-C in acromegaly: correlation with the degree of growth hormone hypersecretion. Journal of Clinical Endocrinology and Metabolism 1988 67 69-73. (Pubitemid 18177118)
9. Elmlinger MW, Kuhnel W, Weber MM & Ranke MB. Reference ranges for two automated chemiluminescent assays for serum insulin-like growth factor I (IGF-I) and IGF-binding protein 3 (IGFBP-3). Clinical Chemistry and Laboratory Medicine 2004 42 654-664. (Pubitemid 38979144)
10. Brugts MP, Ranke MB, Hofland LJ, van der Wansem K, Weber K, Frystyk J, Lamberts SW & Janssen JA. Normal values of circulating insulin-like growth factor-I bioactivity in the healthy population: comparison with five widely used IGF-I immunoassays. Journal of Clinical Endocrinology and Metabolism 2008 93 2539-2545.
11. Chen JW, Ledet T, Orskov H, Jessen N, Lund S, Whittaker J, De Meyts P, Larsen MB, Christiansen JS & Frystyk J. A highly sensitive and specific assay for determination of IGF-I bioactivity in human serum. American Journal of Physiology. Endocrinology and Metabolism 2003 284 E1149-E1155.
12. Ismail AA. A radical approach is needed to eliminate interference from endogenous antibodies in immunoassays. Clinical Chemistry 2005 51 25-26.
13. Selby C. Interference in immunoassay. Annals of Clinical Biochemistry 1999 36 704-721.
14. Emerson JF, Ngo G & Emerson SS. Screening for interference in immunoassays. Clinical Chemistry 2003 49 1163-1169.
15. Ramaeker D, Brannian J, Egland K, McCaul K & Hansen K. When is elevated testosterone not testosterone? When it is an immunoassay interfering antibody. Fertility and Sterility 2008 90 886-888.
16. 125I] testosterone in patient's serum: a problem for the laboratory and the patient. Clinical Chemistry 1996 42 2047-2048.
17. Covinsky M, Laterza O, Pfeifer JD, Farkas-Szallasi T & Scott MG. An IgM lambda antibody to Escherichia coli produces false-positive results in multiple immunometric assays. Clinical Chemistry 2000 46 1157-1161. (Pubitemid 30643319)
18. Ellis MJ & Livesey JH. Techniques for identifying heterophile antibody interference are assay specific: study of seven analytes on two automated immunoassay analyzers. Clinical Chemistry 2005 51 639-641.
19. Kricka LJ. Human anti-animal antibody interferences in immunological assays. Clinical Chemistry 1999 45 942-956.
20. Janssen JA, Blankestijn PJ, Docter R, Blijenberg BG, Splinter TA, van Toor H, Schalekamp MA, Lamberts SW & Krenning EP. Effects of immunoscintigraphy with monoclonal antibodies in assays of hormones and tumour markers. BMJ 1989 298 1511-1513. (Pubitemid 19144582)
21. van Toorenenbergen AW, Hooijkaas H, Heerenbrink GK & Dufour-van den Goorbergh DM. Heterophilic antibody interference in a tryptase immunoassay. Clinical Biochemistry 2008 41 331-334.
22. Kaplan IV & Levinson SS. When is a heterophile antibody not a heterophile antibody? When it is an antibody against a specific immunogen Clinical Chemistry 1999 45 616-618.
23. van Toorenenbergen AW, van Daele PL & Boonstra JG. False-elevated serum tryptase assay result caused by heterophilic antibodies. Journal of Allergy and Clinical Immunology 2005 116 1159-1160.
24. Andersen DC, Koch C, Jensen CH, Skjodt K, Brandt J & Teisner B. High prevalence of human anti-bovine IgG antibodies as the major cause of false positive reactions in two-site immunoassays based on monoclonal antibodies. Journal of Immunoassay and Immunochemistry 2004 25 17-30. (Pubitemid 38292783)
25. Ismail AA & Barth JH. Wrong biochemistry results. BMJ 2001 323 705-706.
26. Talapatra I, Tynnis DJ & Lloyd D. Assay interference of hormones leading to erroneous results: two case reports. Journal, Indian Academy of Clinical Medicine 2007 8 97-98. (Pubitemid 46353893)
27. Ismail AA, Walker PL, Cawood ML & Barth JH. Interference in immunoassay is an underestimated problem. Annals of Clinical Biochemistry 2002 39 366-373.
28. Bjerner J. Human anti-immunoglobulin antibodies interfering in immunometric assays. Scandinavian Journal of Clinical and Laboratory Investigation 2005 65 349-364. (Pubitemid 41134084)
29. Weber TH, Kapyaho KI & Tanner P. Endogenous interference in immunoassays in clinical chemistry. Scandinavian Journal of Clinical and Laboratory Investigation 1990 201 77-82. (Pubitemid 20271063)