Evaluation of the Cepheid Respiratory Syncytial Virus and Influenza Virus A/B real-time PCR analyte specific reagent

Journal of Virological Methods

Volumn 162, Issue 1-2, 2009, Pages 88-90

  Sails, Andrew D ^a   Saunders, David ^a   Airs, Stephanie ^a   Roberts, David ^a   Eltringham, Gary ^a   Magee, John G ^a  

^a NEWCASTLE GENERAL HOSPITAL   (United Kingdom)

Author keywords

Detection; Evaluation; Influenza A B; Real time PCR; RSV

Indexed keywords

ADENOVIRUS; ARTICLE; BOCAVIRUS; CONTROLLED STUDY; CORONAVIRUS; HUMAN; HUMAN METAPNEUMOVIRUS; INFLUENZA VIRUS A; INFLUENZA VIRUS B; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PARAINFLUENZA VIRUS 1; PARAINFLUENZA VIRUS 2; PARAINFLUENZA VIRUS 4; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHINOVIRUS; SENSITIVITY AND SPECIFICITY; VIRAL RESPIRATORY TRACT INFECTION; VIRUS DETECTION;

HUMANS; INDICATORS AND REAGENTS; INFLUENZA A VIRUS; INFLUENZA B VIRUS; INFLUENZA, HUMAN; POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; RESPIRATORY TRACT INFECTIONS; SENSITIVITY AND SPECIFICITY;

ADENOVIRIDAE; BOCAVIRUS; CORONAVIRUS; ORTHOMYXOVIRIDAE; RESPIRATORY SYNCYTIAL VIRUS; RHINOVIRUS; RICE STRIPE VIRUS;

EID: 70349754214     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.07.020     Document Type: Article

References (19)

1. Anon., 2006. Virology Standard Operating Procedure 25.0. Real-Time Quadriplex PCR for the detection of Influenza. Health Protection Agency. http://www.hpa-standardmethods.org.uk/pdf_sops.asp.
2. Aslanzadeh J., Zheng X., Li H., Tetreault J., Ratkiewicz I., Meng S., Hamilton P., and Tang Y.W. Prospective evaluation of rapid antigen tests for diagnosis of respiratory syncytial virus and human metapneumovirus infections. J. Clin. Microbiol. 46 (2008) 1682-1685
3. Barkham T. BioRobot EZ1 workstation compares well with manual spin kits for extraction of viral RNA from sera and saves substantial staff time. J. Clin. Microbiol. 44 (2006) 1598
4. Boivin G., Côté S., Déry P., De Serres G., and Bergeron M.G. Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses. J. Clin. Microbiol. 42 (2004) 45-51
5. Brittain-Long R., Nord S., Olofsson S., Westin J., Anderson L.M., and Lindh M. Multiplex real-time PCR for detection of respiratory tract infections. J. Clin. Virol. 41 (2008) 53-56
6. Curran M.D., Ellis J.S., Wreghitt T.G., and Zambon M.C. Establishment of a UK National Influenza H5 Laboratory Network. J. Med. Microbiol. 56 (2007) 1263-1267
7. Dundas N., Leos N.K., Mitui M., Revell P., and Rogers B.B. Comparison of automated nucleic acid extraction methods with manual extraction. J. Mol. Diagn. 10 (2008) 311-316
8. Goodrich J.S., and Miller M.B. Comparison of Cepheid's analyte-specific reagents with BD directigen for detection of respiratory syncytial virus. J. Clin. Microbiol. 45 (2007) 604-606
9. Gueudin M., Vabret A., Petitjean J., Gouarin S., Brouard J., and Freymuth F. Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay. J. Virol. Methods 109 (2003) 39-45
10. Gunson R.N., Collins T.C., and Carman W.F. Real-time RT-PCR detection of 12 respiratory viral infections in four triplex reactions. J. Clin. Virol. 33 (2005) 341-344
11. Habib-Bein N.F., Beckwith III W.H., Mayo D., and Landry M.L. Comparison of SmartCycler real-time reverse transcription-PCR assay in a public health laboratory with direct immunofluorescence and cell culture assays in a medical center for detection of influenza A virus. J. Clin. Microbiol. 41 (2003) 3597-3601
12. Hymas W.C., and Hillyard D.R. Evaluation of Nanogen MGB Alert Detection Reagents in a multiplex real-time PCR for influenza virus types A and B and respiratory syncytial virus. J. Virol. Methods 156 (2009) 124-128
13. Kuypers J., Wright N., and Morrow R. Evaluation of quantitative and type-specific real-time RT-PCR assays for detection of respiratory syncytial virus in respiratory specimens from children. J. Clin. Virol. 31 (2004) 123-129
14. Leven M. Currently used nucleic acid amplification tests for the detection of viruses and atypicals in acute respiratory infections. J. Clin. Virol. 40 (2007) 259-276
15. Selvarangan R., Abel D., and Hamilton M. Comparison of BD Directigen EZ RSV and Binax NOW RSV tests for rapid detection of respiratory syncytial virus from nasopharyngeal aspirates in a pediatric population. Diagn. Microbiol. Infect. Dis. 62 (2008) 157-161
16. Smith A.B., Mock V., Melear R., Colarusso P., and Willis D.E. Rapid detection of influenza A and B viruses in clinical specimens by Light Cycler real time RT-PCR. J. Clin. Virol. 28 (2003) 51-58
17. Stone B., Burrows J., Schepetiuk S., Higgins G., Hampson A., Shaw R., and Kok T. Rapid detection and simultaneous subtype differentiation of influenza A viruses by real time PCR. J. Virol. Methods 117 (2004) 103-112
18. van Elden L.J.R., Nijhuis M., Schipper P., Schuurman R., and van Loon A.M. Simultaneous detection of influenza viruses A and B using real-time quantitative PCR. J. Clin. Microbiol. 39 (2001) 196-200
19. Woo P.C., Chiu S.S., Seto W.H., and Peiris M. Cost-effectiveness of rapid diagnosis of viral respiratory tract infections in pediatric patients. J. Clin. Microbiol. 35 (1997) 1579-1581