Comparison of BD Directigen™ EZ RSV and Binax NOW® RSV tests for rapid detection of respiratory syncytial virus from nasopharyngeal aspirates in a pediatric population

Diagnostic Microbiology and Infectious Disease

Volumn 62, Issue 2, 2008, Pages 157-161

  Selvarangan, Rangaraj ^a,b   Abel, David ^a   Hamilton, Marilyn ^a,b  

^a CHILDREN S MERCY HOSPITAL   (United States)

^b UNIVERSITY OF MISSOURI KANSAS CITY SCHOOL OF MEDICINE   (United States)

Author keywords

BD Directigen EZ RSV; Binax NOW RSV; Children; Rapid assay; Respiratory syncytial virus; RSV

Indexed keywords

NUCLEIC ACID;

ARTICLE; ASSAY; CHROMATOGRAPHY; CONFIDENCE INTERVAL; CONTROLLED STUDY; DIAGNOSTIC TEST; HUMAN; INTERMETHOD COMPARISON; NASOPHARYNGEAL ASPIRATION; PEDIATRICS; PRIORITY JOURNAL; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TURNAROUND TIME; VIRUS CULTURE; VIRUS INFECTION;

CHILD; CHILD, PRESCHOOL; HUMANS; IMMUNOASSAY; NASOPHARYNX; PREDICTIVE VALUE OF TESTS; REAGENT KITS, DIAGNOSTIC; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TIME FACTORS; VIROLOGY; VIRUS CULTIVATION;

EID: 51649108024     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2008.05.005     Document Type: Article

References (21)

1. Abanses J.C., Dowd M.D., Simon S.D., and Sharma V. Impact of rapid influenza testing at triage on management of febrile infants and young children. Pediatr. Emerg. Care 22 (2006) 145-149
2. Aldous W.K., Gerber K., Taggart E.W., Thomas J., Tidwell D., and Daly J.A. A comparison of Binax NOW to viral culture and direct fluorescent assay testing for respiratory syncytial virus. Diagn. Microbiol. Infect. Dis. 49 (2004) 265-268
3. Barenfanger J., Drake C., Mueller T., Troutt T., O'Brien J., and Guttman K. R-Mix cells are faster, at least as sensitive and marginally more costly than conventional cell lines for the detection of respiratory viruses. J. Clin. Virol. 22 (2001) 101-110
4. Borek A.P., Clemens S.H., Gaskins V.K., Aird D.Z., and Valsamakis A. Respiratory syncytial virus detection by Remel Xpect, Binax Now RSV, Direct Immunofluorescent staining and Tissue culture. J. Clin. Microbiol. 44 (2006) 1105-1107
5. Dunn J.J., Woolstenhulme R.D., Langer J., and Carroll K.C. Sensitivity of respiratory virus culture when screening with R-mix fresh cells. J. Clin. Microbiol. 42 (2004) 79-82
6. Falsey A.R., Hennessey P.A., Formica M.A., Cox C., and Walsh E.E. Respiratory syncytial virus infection in elderly and high-risk adults. N. Engl. J. Med. 352 (2005) 1749-1759
7. Halstead D.C., Todd S., and Fritch G. Evaluation of five methods for respiratory syncytial virus detection. J. Clin. Microbiol. 28 (1990) 1021-1025
8. Jafri H., Ramilo O., Makari D., Charsha-May D., and Romero J. Diagnostic virology practices for respiratory syncytial virus (RSV) and influenza virus among children in the hospital setting: a national survey. Pediatr. Infect. Dis. J. 26 (2007) 956-958
9. Mackie P.L., McCormick E.M., and Williams C. Evaluation of Binax NOW RSV as an acute point-of-care screening test in a paediatric accident and emergency unit. Commun. Dis. Public Health 7 (2004) 328-330
10. Ohm-Smith M.J., Nassos P.S., and Haller B.L. Evaluation of the Binax NOW, BD Directigen, and BD Directigen EZ assays for detection of respiratory syncytial virus. J. Clin. Microbiol. 42 (2004) 2996-2999
11. Paramore L.C., Ciuryla V., Ciesla G., and Liu L. Economic impact of respiratory syncytial virus-related illness in the US: an analysis of national databases. Pharmacoeconomics. 22 (2004) 275-284
12. Reina J., Gonzalez G.M., Ruiz D.G., Padilla E., Ballesteros F., Mari M., and Munar M. Prospective evaluation of a dot-blot enzyme immunoassay (Directigen RSV) for the antigenic detection of respiratory syncytial virus from nasopharyngeal aspirates of paediatric patients. Clin. Microbiol. Infect. 10 (2004) 967-971
13. Sharma V., Dowd M.D., Slaughter A.J., and Simon S.D. Effect of rapid diagnosis of influenza virus type a on the emergency department management of febrile infants and toddlers. Arch. Pediatr. Adolesc. Med. 156 (2002) 41-43
14. Shay D.K., Holman R.C., Newman R.D., Liu L.L., Stout J.W., and Anderson L.J. Bronchiolitis-associated hospitalizations among US children, 1980-1996. JAMA 282 (1999) 1440-1446
15. Slinger R., Milk R., Gaboury I., and Diaz-Mitoma F. Evaluation of the QuickLab RSV test, a new rapid lateral-flow immunoassay for detection of respiratory syncytial virus antigen. J. Clin. Microbiol. 42 (2004) 3731-3733
16. St George K., Patel N.M., Hartwig R.A., Scholl D.R., Jollick Jr. J.A., Kauffmann L.M., Evans M.R., and Rinaldo Jr. C.R. Rapid and sensitive detection of respiratory virus infections for directed antiviral treatment using R-Mix cultures. J. Clin. Virol. 24 (2002) 107-115
17. Thompson W.W., Shay D.K., Weintraub E., Brammer L., Cox N., Anderson L.J., and Fukuda K. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 289 (2003) 179-186
18. Titus M.O., and Wright S.W. Prevalence of serious bacterial infections in febrile infants with respiratory syncytial virus infection. Pediatrics 112 (2003) 282-284
19. Williams K.M., Jackson M.A., and Hamilton M. Rapid Diagnostic Testing for URIs in children: impact on physician decision making and costs. Infect. Med. 19 (2002) 109-111
20. Woo P.C., Chiu S.S., Seto W.H., and Peiris M. Cost-effectiveness of rapid diagnosis of viral respiratory tract infections in pediatric patients. J. Clin. Microbiol. 35 (1997) 1579-1581
21. Zheng X., Quianzon S., Mu Y., and Katz B.Z. Comparison of two new rapid antigen detection assays for respiratory syncytial virus with another assay and shell vial culture. J. Clin. Virol. 31 (2004) 130-133