Overexpression, purification and characterization of organic solvent stable lipase from bacillus licheniformis RSP-09

Journal of Molecular Microbiology and Biotechnology

Volumn 17, Issue 3, 2009, Pages 118-123

  Madan, Bhawna ^a   Mishra, Prashant ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

Bacillus licheniformis; Lipase; Organic solvent tolerance; Overexpression; Purification

Indexed keywords

BACILLUS LICHENIFORMIS RSP 09 LIPASE; BACILLUS PUMILUS B26 LIPASE; BACTERIAL ENZYME; EDETIC ACID; POLYHISTIDINE TAG; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS LICHENIFORMIS; BACILLUS PUMILUS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; ION PAIR CHROMATOGRAPHY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE SENSITIVITY;

BACILLUS; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DETERGENTS; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; KINETICS; LIPASE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SOLVENTS; SUBSTRATE SPECIFICITY; TEMPERATURE;

BACILLUS LICHENIFORMIS; BACILLUS PUMILUS; ESCHERICHIA COLI;

EID: 70349485840     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000208523     Document Type: Article

References (27)

1. Arpigny JS, Jaeger KE: Bacterial lipolytic enzymes: classification and properties. Biochem J 1999; 343: 177-183.
2. Alvarez-Macarie E, Baratti J: Short chain flavor ester synthesis by a new esterase from Bacillus licheniformis . J Mol Catalysis B: Enzym 2000; 10: 377-383.
3. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds): Current Protocols in Molecular Biology, ed 2. New York, Wiley, 1987, vol 1.
4. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254.
5. Castro-Ochoa LD, Rodrýguez-Gomez C, Valerio- Alfaro G, Ros RO: Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11. Enzyme Microb Tech 2005; 37: 648-654.
6. Chand S, Mishra P: Research and applications of microbial enzymes - India's contribution. Adv Biochem Eng/Biotechnol 2003; 85: 95-124.
7. Chen S, Bingzhao S: Purification and properties of enantioselective lipase from a newly isolated Bacillus cereus C71. Process Biochem 2007; 42: 988-994. (Pubitemid 46702766)
8. Furth AJ: Removing unbound detergent from hydrophobic proteins. Anal Biochem 1980; 109: 207-215.
9. Jaeger KE, Eggert T: Lipases for biotechnology. Curr Opin Biotechnol 2002; 13: 390-397.
10. Khyami-Horani H: Thermotolerant strain of Bacillus licheniformis producing lipase. World J Microb Biot 1996; 12: 399-401.
11. Kim HK, Choi HJ, Kim MH, Sohn CB, Oh TK: Expression and characterization of Ca(2+)- independent lipase from Bacillus pumilus B26. Biochim Biophys Acta 2002; 1583: 205-212.
12. Klibanov AM: Improving enzymes by using them in organic solvents. Nature 2001; 409: 241-245.
13. Lima VMG, Krieger N, Mitchell DA, Baratti JC, de Filippis I, Fontana JD: Evaluation of the potential for use in biocatalysis of a lipase from the wild strain of Bacillus megaterium . J Mol Catalysis B: Enzym 2004; 31: 53-61.
14. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685.
15. Ma J, Zhang Z, Wang B, Kong X, Wang Y, Cao S, Feng YN: Overexpression and characterization of a lipase from Bacillus subtilis . Protein Expr Purif 2006; 45: 22-29.
16. Nthangeni MB, Patterton H, Tonder A, Vergeer WP, Litthauer D: Over-expression and properties of a purified recombinant Bacillus licheniformis lipase: a comparative report on Bacillus lipases. Enzyme Microb Technol 2001; 28: 705-712.
17. Reetz MT: Lipases as practical biocatalysts. Current Opin Chem Biol 2002; 6: 145-150.
18. Reetz MT, Jaeger KE: Overexpression, immobilization and biotechnological applications of Pseudomonas lipases. Chem Phys Lipids 1998; 93: 3-14.
19. Rosenau F, Tommassen J, Jaeger KE: Lipase-specific foldases. Chem Biol Chem 2004; 5: 152-161.
20. Sambrook J, Fritsch E, Maniatis T: Molecular Cloning: A Laboratory Manual, ed 2. Cold Spring Harbor, Cold Spring Harbor Laboratory Press, 1989.
21. Sareen R, Bornscheuer UT, Mishra P: Synthesis of kyotorphin precursor by an organic solvent- stable protease from Bacillus licheniformis RSP-09-37. J Mol Catalysis [B] 2004a;32: 1-5.
22. Sareen R, Bornscheuer UT, Mishra P: A microtiter plate assay for the determination of the synthetic activity of protease. Anal Biochem 2004b;333: 193-195.
23. Sareen R, Bornscheuer UT, Mishra P: Cloning, functional expression and characterization of an alkaline protease from Bacillus licheniformis . Biotechnol Lett 2005; 27: 1901-1907.
24. Sareen R, Mishra P: Purification and characterization of organic solvent stable protease from Bacillus licheniformis RSP-09-37. Appl Microbiol Biotechnol 2008; 79: 399-405.
25. Sulong MR, Abdul Rahman RN, Salleh AB, Basri M: A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene. Protein Expr Purif 2006; 49: 190-195.
26. Veith B, Herzberg C, Steckel S, Feesche J, Maurer KH, Ehrenreich P, Baumer S, Henne A, Liesegang H, Merkl R, Ehrenreich A, Gottschalk G: The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential. J Mol Microbiol Biotechnol 2004; 7: 204-211.
27. Winkler UK, Stuckmann M: Glycogen, hyaluronate and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens . J Bacteriol 1979; 138: 663-670.