Development and application of a duplex QPCR for river water samples to monitor the myxozoan parasite Parvicapsula minibicornis

Diseases of Aquatic Organisms

Volumn 86, Issue 1, 2009, Pages 39-50

  Hallett, Sascha L ^a   Bartholomew, Jerri L ^a  

^a OREGON STATE UNIVERSITY   (United States)

Author keywords

Klamath River Salmonids; Monitoring program; Myxozoa; Parvicapsula minibicornis; Quantitative PCR; River water samples

Indexed keywords

RIBOSOME RNA;

ABUNDANCE; BIOASSAY; DNA; ENDEMIC SPECIES; GENE; HEALTH IMPACT; HEALTH MONITORING; HOST-PARASITE INTERACTION; INHIBITION; LIFE CYCLE; PARASITIC DISEASE; POLYMERASE CHAIN REACTION; PROBE; PROTOZOAN; QUANTITATIVE ANALYSIS; RIVER WATER; RNA; SALMONID; SAMPLING; SEASONAL VARIATION; SPATIAL DISTRIBUTION; SPORE; TRIBUTARY;

ANIMAL; ANIMAL PARASITOSIS; ARTICLE; FISH DISEASE; GENETICS; MICROBIOLOGY; MYXOZOA; PARASITOLOGY; PHYSIOLOGY; POLYMERASE CHAIN REACTION; PREVALENCE; RIVER; SALMONID; SEASON; SENSITIVITY AND SPECIFICITY;

ANIMALS; FISH DISEASES; MYXOZOA; PARASITIC DISEASES, ANIMAL; POLYMERASE CHAIN REACTION; PREVALENCE; RIVERS; RNA, RIBOSOMAL; SALMONIDAE; SEASONS; SENSITIVITY AND SPECIFICITY;

KLAMATH RIVER; NORTH AMERICA; PACIFIC NORTHWEST; UNITED STATES;

BACTERIA (MICROORGANISMS); MYXOZOA; PARVICAPSULA MINIBICORNIS; SALMONIDAE;

EID: 70349472762     PISSN: 01775103     EISSN: 16161580     Source Type: Journal    
DOI: 10.3354/dao02104     Document Type: Article

References (31)

1. Atkinson SD, Jones SMR, Bartholomew JL (2007) Parvicap- sula minibicornis: a myxozoan species complex? In: Coluzzi M (ed) Parassitologia. 7th Int Symp Fish Parasites. Lombardo Editore, Rome, p 139
2. Audemard C, Reece KS, Burreson EM (2004) Real-time PCR for detection and quantification of the protistan parasite Perkinsus marinus in environmental waters. Appl Environ Microbiol 70:6611-6618
3. Audemard C, Ragone Calvo LM, Paynter KT, Reece KS, Burreson EM (2006) Real-time PCR investigation of parasite ecology: in situ determination of oyster parasite Perkinsus marinus transmission dynamics in lower Chesapeake Bay.Parasitology 132:827-842
4. Bartholomew JL, Rohovec JS, Fryer JL (1989) Ceratomyxa shasta, a myxosporean parasite of salmonids. Fish Disease Leaflet 80, US Fish and Wildlife Service Bartholomew JL, Whipple MJ, Stevens DG, Fryer JL (1997) The life cycle of Ceratomyxa shasta, a myxosporean parasite of salmonids, requires a freshwater polychaete as an alternate host. J Parasitol 83:859-868
5. Bartholomew JL, Atkinson SD, Hallett SL (2006) Involvement of Manayunkia speciosa (Annelida: Polychaeta: Sabelli- dae) in the life cycle of Parvicapsula minibicornis, a myxo- zoan parasite of Pacific salmon. J Parasitol 92:742-748
6. Bartholomew JL, Atkinson SD, Hallett SL, Zielinski CM, Foott JS (2007) Distribution and abundance of the salmonid parasite Parvicapsula minibicornis (Myxozoa) in the Klamath River Basin (Oregon-California, USA). Dis Aquat Org78:137-146
7. Behets J, Declerck P, Delaedt Y, Verelst L, Ollevier F (2007) A duplex real-time PCR assay for the quantitative detection of Naegleria fowleri in water samples. Water Res 41: 118-126
8. Bustin SA (ed) (2004) A-Z of quantitative PCR. International University Line, La Jolla, CA
9. Bustin SA, Nolan T (2004) Data analysis and interpretation. In: Bustin, SA (ed) (2004) A-Z of quantitative PCR. International University Line, La Jolla, CA, p 439-492
10. Conrad JF, Decew M (1966) First report of Ceratomyxa in juvenile salmonids in Oregon. Prog Fish-Cult 28:238
11. Foott JS, Harmon R, Stone R (2004a) FY2003 investigational report: abundance of Ceratomyxa shasta in Iron Gate and Copco Reservoirs. US Fish and Wildlife Service California- Nevada Fish Health Center, Anderson, CA
12. Foott JS, Harmon R, Stone R (2004b) Effect of water temperature on non-specific immune function and ceratomyxosis in juvenile Chinook salmon and steelhead from the Kla- math River. Calif Fish Game 90:71-84
13. Foott JS, Stone R, Wiseman E, True K, Nichols K (2007) Longevity of Ceratomyxa shasta and Parvicapsula mini bicornis actinospore infectivity in the Klamath River. J Aquat Anim Health 19:77-83
14. Guy RA, Payment P, Krull UJ, Horgen PA (2003) Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage. Appl Environ Microbiol 69:5178-5185
15. Hallett SL, Bartholomew JL (2006) Application of a real-time PCR assay to detect and quantify the myxozoan parasite Ceratomyxa shasta in river water samples. Dis Aquat Org 71:109-118
16. Haugland RA, Siefring SC, Wymer LJ, Brenner KP, Dufour AP (2005) Comparison of Enterococcus measurements in freshwater at two recreational beaches by quantitative polymerase chain reaction and membrane filter culture analysis. Water Res 39:559-568
17. Jiang J, Alderisio KA, Singh A, Xiao L (2005) Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors. Appl Environ Microbiol 71:1135-1141
18. Jones SRM, Prosperi-Porta G, Dawe SC, Barnes DP (2003) Distribution, prevalence, and severity of Parvicapsula minibicornis infections among anadromous salmonids in the Fraser River, British Columbia, Canada. Dis Aquat Org 54:49-52
19. Kent ML, Whitaker DJ, Dawe SC (1997) Parvicapsula minibi- cornis n. sp. (Myxozoa, Myxosporea) from the kidney of sockeye salmon (Oncorhynchus nerka) from British Columbia, Canada. J Parasitol 83:1153-1156
20. McBeath AJA, Penston MJ, Snow M, Cook PF, Bricknell IR, Cunningham CO (2006) Development and application of real-time PCR for specific detection of Lepeophtheirus salmonis and Caligus elongatus larvae in Scottish plankton samples. Dis Aquat Org 73:141-150
21. National Research Council (2004) Endangered and threatened fish in the Klamath River basin: causes of decline and strategies for recovery. The National Academies Press, Washington, DC
22. Nichols K, Foott JS (2006) FY2004 investigational report: health monitoring of juvenile Klamath River Chinook salmon. US Fish & Wildlife Service California-Nevada Fish Health Center, Anderson, CA
23. Nichols K, True K (2007) FY 2006 investigational report: monitoring incidence and severity of Ceratomyxa shasta and Parvicapsula minibicornis infections in juvenile Chinook salmon (Oncorhynchus tshawytscha) and coho salmon (Oncorhynchus kisutch) in the Klamath River, 2006. US Fish & Wildlife Service California-Nevada Fish Health Center, Anderson, CA
24. Nichols K, True K, Wiseman E, Foott JS (2007) FY2005 investigational report: incidence of Ceratomyxa shasta and Parvicapsula minibicornis infections by QPCR and histology in juvenile Klamath River Chinook salmon. US Fish & Wildlife Service California-Nevada Fish Health Center, Anderson, CA
25. Phillips JM (2004) Real-time RT-PCR: what lies beneath the surface. In: Bustin SA (ed) A-Z of quantitative PCR. International University Line, La Jolla, CA, p 47-85
26. Schafer WE (1968) Studies on the epizootiology of the myxosporidian Ceratomyxa shasta Noble. Calif Fish Game 54: 90-99
27. St-Hilaire S, Boichuk M, Barnes D, Higgins M and others (2002) Epizootiology of Parvicapsula minibicornis in Fraser River sockeye salmon, Oncorhynchus nerka (Walbaum). J Fish Dis 25:107-120
28. Stocking RW, Holt RA, Foott JS, Bartholomew JL (2006) Spatial and temporal occurrence of the salmonid parasite Ceratomyxa shasta (Myxozoa) in the Oregon-California Kla- math River basin. J Aquat Anim Health 18:194-202
29. Stone R, Foott JS, Fogerty R (2008) FY2006 investigationalreport: comparative susceptibility to infection and disease from Ceratomyxa shasta and Parvicapsula minibicornis in Klamath River basin juvenile Chinook, coho, and steel- head populations. US Fish & Wildlife Service California- Nevada Fish Health Center, Anderson, CA
30. Swango KL, Timken MD, Chong MD, Buoncristiani MR (2006) A quantitative PCR assay for the assessment of DNA degradation in forensic samples. Forensic Sci Int 158:14-26
31. True K, Purcell M, Foott JS (2009) Development and validation of a quantitative PCR to detect Parvicapsula minibicornis and comparison to histologically ranked infection of juvenile Chinook salmon, Oncorhynchus tshawytscha (Walbaum), from the Klamath River, USA. J Fish Dis 32:183-192