Engineering monomeric streptavidin and its ligands with infinite affinity in binding but reversibility in interaction

Proteins: Structure, Function and Bioinformatics

Volumn 77, Issue 2, 2009, Pages 404-412

  Wu, Sau Ching ^a   Ng, Kenneth K S ^a   Wong, Sui Lam ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

Bacillus subtilis; Biotin; Biotin ligase; Biotinylation tag; Cysteine; Disulfide bond; Immobilization; Protein engineering; Secretion; Staphylokinase

Indexed keywords

BIOTIN; CYSTEINE; LYSINE; STREPTAVIDIN;

ARTICLE; BIOTINYLATION; COVALENT BOND; DISULFIDE BOND; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BINDING SITES; BIOTIN; BIOTINYLATION; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; LIGANDS; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; STREPTAVIDIN; STRUCTURE-ACTIVITY RELATIONSHIP;

BACILLUS SUBTILIS;

EID: 70349464722     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.22446     Document Type: Article

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