Characterization of the SRP68/72 interface of human signal recognition particle by systematic site-directed mutagenesiss

Protein Science

Volumn 18, Issue 10, 2009, Pages 2183-2195

  Iakhiaeva, Elena ^a   Hinck, Cynthia S ^b   Hinck, Andrew P ^b   Zwieb, Christian ^a  

^a UNIVERSITY OF TEXAS HEALTH CENTER   (United States)

^b Mays Cancer Center at UT Health San Antonio   (United States)

Author keywords

Protein sorting; Protein protein interactions; Signal recognition particle; SRP

Indexed keywords

AMINO ACID; ASPARTIC ACID; HETERODIMER; SIGNAL RECOGNITION PARTICLE; SIGNAL RECOGNITION PARTICLE 68; SIGNAL RECOGNITION PARTICLE 72; TETRATRICOPEPTIDE REPEAT PROTEIN; UNCLASSIFIED DRUG; UREA;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DENATURATION; ESCHERICHIA COLI; HUMAN; IONIC STRENGTH; MUTAGENESIS; PREDICTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE; RECOGNITION; SIGNAL TRANSDUCTION; TETRATRICOPEPTIDE REPEAT;

BINDING SITES; HUMANS; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; MUTATION; PROTEIN BINDING; SIGNAL RECOGNITION PARTICLE; UREA;

ESCHERICHIA COLI; EUKARYOTA;

EID: 70349454222     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.232     Document Type: Article

References (34)

1. Doudna JA, Batey RT (2004) Structural insights into the signal recognition particle. Annu Rev Biochem 73:539-557. (Pubitemid 39050379)
2. Halic M, Beckmann R (2005) The signal recognition particle and its interactions during protein targeting. Curr Opin Struct Biol 15:116-125. (Pubitemid 40249518)
3. Luirink J, Sinning I (2004) SRP-mediated protein targeting: structure and function revisited. Biochim Biophys Acta 1694:17-35. (Pubitemid 39535059)
4. Shan SO, Walter P (2005) Co-translational protein targeting by the signal recognition particle. FEBS Lett 579:921-926. (Pubitemid 40361866)
5. Walter P, Blobel G (1983) Disassembly and reconstitution of signal recognition particle. Cell 34:525-533.
6. Andrews DW, Walter P, Ottensmeyer FP (1987) Evidence for an extended 7SL RNA structure in the signal recognition particle. EMBO J 6:3471-3477.
7. Weichenrieder O, Wild K, Strub K, Cusack S (2000) Structure and assembly of the Alu domain of the mammalian signal recognition particle. Nature 408:167-173. (Pubitemid 30839411)
8. Halic M, Becker T, Pool MR, Spahn CM, Grassucci RA, Frank J, Beckmann R (2004) Structure of the signal recognition particle interacting with the elongation-arrested ribosome. Nature 427:808-814. (Pubitemid 38297736)
9. Walter P, Ibrahimi I, Blobel G (1981) Translocation of proteins across the endoplasmic reticulum: signal recognition protein (srp) binds to in-vitro assembled polysomes synthesizing secretory protein. J Cell Biol 91:545-550. (Pubitemid 12215642)
10. Andersen ES, Rosenblad MA, Larsen N, Westergaard JC, Burks J, Wower IK, Wower J, Gorodkin J, Samuelsson T, Zwieb C (2006) The tmRDB and SRPDB resources. Nucl Acids Res 34:D163-D168.
11. Zwieb C, Eichler J (2002) Getting on target: the archaeal signal recognition particle. Archaea 1:27-34. (Pubitemid 38871628)
12. Zwieb C, Van Nues RW, Rosenblad MA, Brown JD, Samuelsson T (2005) A nomenclature for all signal recognition particle RNAs. RNA 11:7-13. (Pubitemid 40041568)
13. Politz JC, Yarovoi S, Kilroy SM, Gowda K, Zwieb C, Pederson T (2000) Signal recognition particle components in the nucleolus. Proc Natl Acad Sci USA 97:55-60. (Pubitemid 30055783)
14. Brown JD, Hann BC, Medzihradszky KF, Niwa M, Burlingame AL, Walter P (1994) Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression. EMBO J 13:4390-4400. (Pubitemid 24295211)
15. Van Nues RW, Leung E, Mcdonald JC, Dantuluru I, Brown JD (2008) Roles for Srp72p in assembly, nuclear export and function of the signal recognition particle. RNA Biol 5:73-83. (Pubitemid 351881375)
16. Scoulica E, Krause E, Meese K, Dobberstein B (1987) Disassembly and domain structure of the proteins in the signal-recognition particle. Eur J Biochem 163:519-528. (Pubitemid 17036349)
17. Lustig Y, Goldshmidt H, Uliel S, Michaeli S (2005) The Trypanosoma brucei signal recognition particle lacks the Alu-domain-binding proteins: purification and functional analysis of its binding proteins by RNAi. J Cell Sci 118:4551-4562. (Pubitemid 41556351)
18. Lakkaraju AK, Luyet PP, Parone P, Falguieres T, Strub K (2007) Inefficient targeting to the endoplasmic reticulum by the signal recognition particle elicits selective defects in post-ER membrane trafficking. Exp Cell Res 313:834-847. (Pubitemid 46198825)
19. Yin J, Iakhiaeva E, Menichelli E, Zwieb C (2007) Identification of the RNA binding regions of SRP68/72 and SRP72 by systematic mutagenesis of human SRP RNA. RNA Biol 4:154-159. (Pubitemid 351237916)
20. Menichelli E, Isel C, Oubridge C, Nagai K (2007) Protein- induced conformational changes of RNA during the assembly of human signal recognition particle. J Mol Biol 367:187-203. (Pubitemid 46274718)
21. Iakhiaeva E, Wower J, Wower IK, Zwieb C (2008) The 5e motif of eukaryotic signal recognition particle RNA contains a conserved adenosine for the binding of SRP72. RNA 14:1143-1153. (Pubitemid 351717494)
22. Iakhiaeva E, Bhuiyan SH, Yin J, Zwieb C (2006)Protein SRP68 of human signal recognition particle: identification of the RNA and SRP72 binding domains. Protein Sci 12:467-468. (Pubitemid 43800002)
23. Iakhiaeva E, Yin J, Zwieb C (2005) Identification of an RNA-binding domain in human SRP72. J Mol Biol 345:659-666. (Pubitemid 39600924)
24. Scheufler C, Brinker A, Bourenkov G, Pegoraro S, Moroder L, Bartunik H, Hartl FU, Moarefi I (2000) Structure of TPR domain-peptide complexes: critical elements in the assembly of the Hsp70-Hsp90 multichaperone machine. Cell 101:199-210. (Pubitemid 32004747)
25. Mcguffin LJ, Bryson K, Jones DT (2000) The PSIPRED protein structure prediction server. Bioinformatics 16:404-405. (Pubitemid 30417087)
26. Wilce MC, Feil SC, Board PG, Parker MW (1994) Crystallization and preliminary x-ray diffraction studies of a glutathione S-transferase from the Australian sheep blowfly, Lucilia cuprina. J Mol Biol 236:1407-1409. (Pubitemid 24147462)
27. Bennion BJ, Daggett V (2003) The molecular basis for the chemical denaturation of proteins by urea. Proc Natl Acad Sci USA 100:5142-5147. (Pubitemid 36542672)
28. Blatch GL, Lassle M (1999) The tetratricopeptide repeat: a structural motif mediating protein-protein interactions. Bioessays 21:932-939.
29. Das AK, Cohen PW, Barford D (1998) The structure of the tetratricopeptide repeats of protein phosphatase 5: implications for TPR-mediated protein-protein interactions. EMBO J 17:1192-1199.
30. Zarrinpar A, Bhattacharyya RP, Lim WA (2003) The structure and function of proline recognition domains. Sci STKE 2003: RE8.
31. Mcginnis S, Madden TL (2004) BLAST: at the core of a powerful and diverse set of sequence analysis tools. Nucl Acids Res 32:W20-5.
32. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucl Acids Res 32:1792-1797. (Pubitemid 38832724)
33. Clamp M, Cuff J, Searle SM, Barton GJ (2004) The Jalview Java alignment editor. Bioinformatics 20:426-427.
34. Cole C, Barber JD, Barton GJ (2008) The Jpred 3 secondary structure prediction server. Nucl Acids Res 36:W197-W201.