Phage display of functional human TNF-α converting enzyme catalytic domain: A rapid method for the production of stabilized proteolytic proteins for assay development and high-throughput screening

Journal of Biomolecular Screening

Volumn 7, Issue 5, 2002, Pages 433-440

  Chen, Yangde ^a   Diener, Katrina ^a   Patel, Indravadan R ^a   Kawooya, John K ^a   Martin, Gary A ^a   Yamdagni, Preeti ^a   Zhang, Xin ^a   Sandrasagra, Anthony ^a   Sahasrabudhe, Sudhir ^a   Busch, Steven J ^a,b  

^a Avenus Pharmaceuticals   (United States)

^b SANOFI   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ADAM PROTEIN; ENZYME INHIBITOR; GLUTATHIONE TRANSFERASE; METALLOPROTEINASE; PEPTIDE LIBRARY; PROTEIN; RECOMBINANT PROTEIN; TUMOR NECROSIS FACTOR ALPHA CONVERTING ENZYME; TUMOR NECROSIS FACTOR-ALPHA CONVERTASE;

ARTICLE; BACULOVIRUS; BIOASSAY; COMBINATORIAL CHEMISTRY; DRUG ANTAGONISM; DRUG SCREENING; ENZYME ACTIVE SITE; ENZYME STABILITY; ESCHERICHIA COLI; GENETICS; HUMAN; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING;

ADAM PROTEINS; BACULOVIRIDAE; BIOLOGICAL ASSAY; CATALYTIC DOMAIN; COMBINATORIAL CHEMISTRY TECHNIQUES; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; GLUTATHIONE TRANSFERASE; HUMANS; METALLOENDOPEPTIDASES; PEPTIDE LIBRARY; PROTEIN ENGINEERING; PROTEINS; RECOMBINANT PROTEINS;

EID: 0642370953     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/108705702237675     Document Type: Article

References (25)

1. Blake RA, Rauch CT, Kozlosky CJ, Peschon JJ, Slack JL, Wolfson MF, et al: Metalloproteinase disintegrin that releases tumour-necrosis factor-α from cells. Nature 1997;385:729-733.
2. Moss ML, Jin SL, Milla ME, Burkhart W, Carter HL, Chen WJ, et al: Cloning of a disintegrin metalloproteinase that processes precursor tumor-necrosis factor-a. Nature I997;385:733-736.
3. Blobel CP: Metalloprotease-disintegrins: links to cell adhesion and cleavage of TNFα and notch. Cell 1997;90:589-592.
4. Wolfsberg TG, Straight PD, Gerena R, Huovila AP, Primakoff P, Myles DG, et al: ADAM, a widely distributed and developmentally regulated gene family encoding membrane proteins with a disintegrin and metalloprotease domain. Dev Biol 1995;169:378-383.
5. Buxbaum JD, Liu KN, Luo Y, Slack JL, Stocking KL, Peschon JJ, et al: Evidence that tumor necrosis factor α converting enzyme is involved in regulated a-secretase cleavage of the Alzheimer amyloid protein precursor. J Biol Chem 1998;273:27765-27767.
6. Clarke HRG, Wolfson MF, Rauch CT, Castner BJ, Huang CP, Gerhart MJ, et al: Expression and purification of correctly processed, active human TACE catalytic domain in Saccharomyces cerevisiae. Protein Expression and Purification 1998;13:104-110.
7. Patel IR, Attur MG, Patel RN, Stuchin SA, Abagyan RA, Abramson SB, et al: TNF-α convertase enzyme from human arthritis-affected cartilage: isolation of cDNA differential display, expression of the active enzyme, and regulation of TNF-α. J Immunol 1998;160:4570-4579.
8. Smith GP: Filamentous fusion phage: novel expression vectors that display cloned antigen on the viron surface. Science 1985;228:1315-1317.
9. McCafferty J, Jackson RH, Chiswell DJ: Phage-enzymes: expression and affinity chromatography of functional alkaline phosphatase on the surface of bacteriophage. Protein Eng 1991 ;4:955-961.
10. Corey DR, Shiau AK, Yang Q, Janowski BA, Craik CS: Trypsin display on the surface of bacteriophage. Gene 1993;128:129-134.
11. Soumillion P, Jespers L, Bouchet M, Marchand BJ, Winter G, Fastrez J: Selection of β-lactamase on filamentous bacteriophage by catalytic activity. J Mol Biol 1994;237:415-422.
12. Stratton-Thomas JR, Min HY, Kaufman SE, Chiu CY, Mullenbach GT, Rosenberg S: Yeast expression and phagemid display of the human urokinase activator epidermal growth factor-like domain. Protein Eng 1995;8:463-470.
13. Tanaka AS, Sampaio CAM, Fritz H, Auerswald EA: Functional display and expression of chicken cyctatin using a phagemid system. Biochem Biophys Res Commun 1995;214:389-395.
14. Maenaka K, Kuruta M, Tsumoto K, Watanabe K, Ueda Y, Kumagai I: A stablephage-display system using a phagemid vector-phage display of hen egg-white lysozyme (HEL), Escherichia coli alkaline phosphatase, and anti-HEL monoclonal antibody, HYHEL 10. Biochem Biophys Res Commun 1996;218:682-687.
15. Lasters I, Van HN, Lijnen HR, Collen D, Jespers L: Enzymatic properties of phage-displayed fragments of human plasminogen. Eur J Biochem 1997:244:946-952.
16. Wirsching F, Opitz T, Dietrich R, Schwienhorst A: Display of functional thrombin inhibitor hirudin on the surface of phage M13. Gene 1997;204:177-84.
17. Lucie MR, Forbes BE, Grosvenor SE, Carr JM, Wallace JC, Forsberg G: Serection in Escherichia coli und phage-display of recombinant insulinlike growth factor binding protein-2. J Biotechnol 1998;61:95-108.
18. Crameri R, Walter G: Selective enrichment and high-throughput screening of phage surface-displayed cDNA libraries from complex allergenic systems. Combinatorial Chemistry & High Throughput Screening 1999;2:63-72.
19. Lemaitre V, Jungblutli A, Eeckhout Y: The recombinant catalytic domain of mouse coIlagenase-3 depolymerizes type I collagen by cleaving its aminotelopeptides. Biochem Biophys Res Commun 1997;230:202-205.
20. Sato H, Kinoshita T, Takino T, Nakayama K, Sciki M: Activation of a recombinant membrane type 1-matrix metalloproteinase (MT1-MMP) by furin and its interaction with tissue inhibitor of metalloproteinase (TIMP)-2. FEES Lett 1996;393:101-104.
21. Miller CG: Protein degradation and proteolytic modification. In Neihardt FC (ed): Escherichia coli and Salmonella: Cellular and Molecular Biology. Washington, DC: ASM Press, 1996:938-954.
22. Oliver D: Periplasm. In Neihardt FC (ed): Escherichia coli and Salmonella: Cellular and Molecular Biology. Washington, DC: ASM Press, 1996:88-103.
23. Armstrong N, Adey NB, McConnell SJ, Kay BK: Vectors for phage display. In Kay B, Winter J, McCafferty JE (eds), Phage Display of Peptides and Proteins: A Laboratory Manual. San Diego: Academic Press, 1996:450.
24. Schwind P, Kramer H, Kremser A, Ramsberger U: Subtilisin removes the surface layer of the phage fd coat. European J Biochem 1992;210:431436.
25. Kay B, Winter J, McCafferty J (eds): Phage Display of Peptides and Proteins: A Laboratory Manual. San Diego: Academic Press, 1996.