RNAi in the malaria vector, Anopheles gambiae

Methods in Molecular Biology

Volumn 555, Issue , 2009, Pages 63-75

  Catteruccia, Flaminia ^a   Levashina, Elena A ^b  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b INSTITUT DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

Author keywords

immunoblotting; Malaria; mosquito; q PCR; RNAi; transgenesis

Indexed keywords

ANOPHELES GAMBIAE; HEXAPODA; PLASMODIUM PARASITES;

DOUBLE STRANDED RNA; PROTEIN;

ANIMAL; ANOPHELES GAMBIAE; ARTICLE; GENE; GENETIC TRANSCRIPTION; GENETICS; HUMAN; INJECTION; INVERTED REPEAT; MALARIA; METABOLISM; MOLECULAR CLONING; PLASMID; RNA INTERFERENCE; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; ANOPHELES GAMBIAE; CLONING, MOLECULAR; GENES, INSECT; HUMANS; INJECTIONS; INVERTED REPEAT SEQUENCES; MALARIA; PLASMIDS; PROTEINS; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; TRANSCRIPTION, GENETIC;

EID: 70349330907     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-295-7_5     Document Type: Article

References (9)

1. van Dijk, M. R., Waters, A. P., and Janse, C. J. (1995) Stable transfection of malaria parasite blood stages Science 268, 1358-1362.
2. Blandin, S., Moita, L. F., K̈ocher, T., Wilm, M., Kafatos, F. C., and Levashina, E. A. (2002) Reverse genetics in the mosquito Anopheles gambiae: targeted disruption of the Defensin gene EMBO Rep 3, 852-856. (Pubitemid 35154110)
3. Handler, A. M., and Harrell, R. A., 2nd (1999) Germline transformation of Drosophila melanogaster with the piggy-Bac transposon vector Insect Mol Biol 8, 449-457.
4. Brown, A. E., Bugeon, L., Crisanti, A., and Catteruccia, F.(2003) Stable and heritable gene silencing in the malaria vector Anopheles stephensi Nucleic Acids Res 31, e85.
5. Brown, A. E., Crisanti, A., and Catteruccia, F.(2003) Comparative analysis of DNA vectors at mediating RNAi in Anopheles mosquito cells and larvae J Exp Biol 206, 1817-1823. (Pubitemid 36715025)
6. Henschel, A., Buchholz, F., and Habermann, B.(2004) DEQOR: a web-based tool for the design and quality control of siRNAs Nucleic Acids Res 32, W113-W120. (Pubitemid 38997311)
7. Catteruccia, F., Nolan, T., Loukeris, T. G., Blass, C., Savakis, C., Kafatos, F. C., and Crisanti, A.(2000) Stable germline transformation of the malaria mosquito Anopheles stephensi Nature 405, 959-962. (Pubitemid 30435053)
8. Lobo, N. F., Clayton, J. R., Fraser, M. J., Kafatos, F. C., and Collins, F. H.(2006) High efficiency germ-line transformation of mosquitoes Nat Protoc 1, 1312-1317.
9. Frolet, C., Thoma, M., Blandin, S., Hoffmann, J. A., and Levashina, E. A. (2006) Boosting NF-kappaB-dependent basal immunity of Anopheles gambiae aborts development of Plasmodium berghei. Immunity 25, 677-685. (Pubitemid 44529039)