Membrane cholesterol depletion from live cells enhances the function of human serotonin1A receptors

Biochemical and Biophysical Research Communications

Volumn 389, Issue 2, 2009, Pages 333-337

  Prasad, Rajesh ^a   Paila, Yamuna Devi ^a   Jafurulla, Md ^a   Chattopadhyay, Amitabha ^a  

^a CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

Fluorescence anisotropy; G protein coupling; Ligand binding activity; Membrane cholesterol; Serotonin1A receptor

Indexed keywords

CHOLESTEROL; GUANINE NUCLEOTIDE BINDING PROTEIN; MEMBRANE LIPID; PHOSPHOLIPID; SEROTONIN 1A RECEPTOR;

ANIMAL CELL; ANISOTROPY; ARTICLE; BINDING SITE; CHOLESTEROL DEPLETION; CONTROLLED STUDY; DEPLETION; MEMBRANE BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; RECEPTOR BINDING;

ANIMALS; CELL MEMBRANE; CHO CELLS; CHOLESTEROL; CRICETINAE; CRICETULUS; HUMANS; LIGANDS; RECEPTOR, SEROTONIN, 5-HT1A;

EID: 70349322386     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.08.148     Document Type: Article

References (35)

1. Simons K., and Ikonen E. How cells handle cholesterol. Science 290 (2000) 1721-1726
2. Mouritsen O.G., and Zuckermann M.J. What's so special about cholesterol?. Lipids 39 (2004) 1101-1113
3. Mukherjee S., and Maxfield F.R. Membrane domains. Annu. Rev. Cell Dev. Biol. 20 (2004) 839-866
4. Jacobson K., Mouritsen O.G., and Anderson R.G.W. Lipid rafts: at a crossroad between cell biology and physics. Nat. Cell Biol. 9 (2007) 7-14
5. Simons K., and van Meer G. Lipid sorting in epithelial cells. Biochemistry 27 (1988) 6197-6202
6. Simons K., and Toomre D. Lipid rafts and signal transduction. Nat. Rev. Mol. Cell Biol. 1 (2000) 31-39
7. Goluszko P., and Nowicki B. Membrane cholesterol: a crucial molecule affecting interactions of microbial pathogens with mammalian cells. Infect. Immun. 73 (2005) 7791-7796
8. Riethmüller J., Riehle A., Grassmé H., and Gulbins E. Membrane rafts in host-pathogen interactions. Biochim. Biophys. Acta 1758 (2006) 2139-2147
9. Pucadyil T.J., and Chattopadhyay A. Cholesterol: a potential therapeutic target in Leishmania infection?. Trends Parasitol. 23 (2007) 49-53
10. Pucadyil T.J., and Chattopadhyay A. Role of cholesterol in the function and organization of G-protein coupled receptors. Prog. Lipid Res. 45 (2006) 295-333
11. 1A receptor: a representative member of the serotonin receptor family. Cell. Mol. Neurobiol. 25 (2005) 553-580
12. Schöneberg T., Hofreiter M., Schulz A., and Römpler H. Learning from the past: evolution of GPCR functions. Trends Pharmacol. Sci. 28 (2007) 117-121
13. 1A-receptors. Prog. Neurobiol. 81 (2007) 133-178
14. Gingrich J.A., and Hen R. Dissecting the role of the serotonin system in neuropsychiatric disorders using knockout mice. Psychopharmacology 155 (2001) 1-10
15. 1A receptor knockout mouse as a genetic model of anxiety. Eur. J. Pharmacol. 463 (2003) 177-184
16. 1A receptor heterologously expressed in CHO cells. Biosci. Rep. 24 (2004) 101-115
17. 1A receptor is impaired in cellular model of Smith-Lemli-Optiz syndrome. Biochim. Biophys. Acta 1778 (2008) 1508-1516
18. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., and Klenk D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150 (1985) 76-85
19. Amundson D.M., and Zhou M. Fluorometric method for the enzymatic determination of cholesterol. J. Biochem. Biophys. Methods 38 (1999) 43-52
20. McClare C.W.F. An accurate and convenient organic phosphorus assay. Anal. Biochem. 39 (1971) 527-530
21. 1A receptors. Biochim. Biophys. Acta 1778 (2008) 2022-2025
22. Kuhry J.-G., Duportail G., Bronner C., and Laustriat G. Plasma membrane fluidity measurements on whole living cells by fluorescence anisotropy of trimethylammoniumdiphenylhexatriene. Biochim. Biophys. Acta 845 (1985) 60-67
23. Lakowicz J.R. Principles of Fluorescence Spectroscopy. third ed. (2006), Springer, New York
24. Zidovetzki R., and Levitan I. Use of cyclodextrins to manipulate plasma membrane cholesterol content: evidence, misconceptions and control strategies. Biochim. Biophys. Acta 1768 (2007) 1311-1324
25. 1A receptors to inhibitory G protein alpha-subunits and lack of coupling to Gs alpha. Biochemistry 32 (1993) 11064-11073
26. 1A receptors from bovine hippocampus by an agonist and an antagonist. FEBS Lett. 457 (1999) 389-392
27. Gudi S., Nolan J.P., and Frangos J.A. Modulation of GTPase activity of G proteins by fluid shear stress and phospholipid composition. Proc. Natl. Acad. Sci. USA 95 (1998) 2515-2519
28. Ropero S., Chiloeches A., Montes A., and Toro-Nozal M.J. Cholesterol cell content modulates GTPase activity of G proteins in GH4C1 cell membranes. Cell. Signal. 15 (2003) 131-138
29. Prendergast F.G., Haugland R.P., and Callahan P.J. 1-[4-(Trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene: synthesis, fluorescence properties, and use as a fluorescence probe of lipid bilayers. Biochemistry 20 (1981) 7333-7338
30. Kuhry J.-G., Fonteneau P., Duportail G., Maechling C., and Laustriat G. TMA-DPH: a suitable fluorescence polarization probe for specific plasma membrane fluidity studies in intact living cells. Cell Biophys. 5 (1983) 129-140
31. Sojcic Z., Toplak H., Zuehlke R., Honegger U.E., Bühlmann R., and Wiesmann U.N. Cultured human skin fibroblasts modify their plasma membrane lipid composition and fluidity according to growth temperature suggesting homeoviscous adaptation at hypothermic (30 °C) but not at hyperthermic (40 °C) temperatures. Biochim. Biophys. Acta 1104 (1992) 31-37
32. Kaiser R.D., and London E. Location of diphenylhexatriene (DPH) and its derivatives within membranes: comparison of different fluorescence quenching analyses of membrane depth. Biochemistry 37 (1998) 8180-8190
33. 1A receptors from bovine hippocampus. Biochim. Biophys. Acta 1663 (2004) 188-200
34. 1A receptor in the plasma membrane of living cells. Biochim. Biophys. Acta 1768 (2007) 655-668
35. 2-adrenergic receptor. Structure 16 (2008) 897-905