Fluorescence optical detection in situ for real-time monitoring of cytochrome P450 enzymatic activity of liver cells in multiple microfluidic devices

Biotechnology and Bioengineering

Volumn 104, Issue 3, 2009, Pages 516-525

  Jong, Hwan Sung ^a   Choi, Jong Ryul ^b   Kim, Donghyun ^b   Shuler, Michael L ^a  

^a Cornell University   (United States)

^b Yonsei University   (South Korea)

Author keywords

Cell culture analog; Fluorescence optical detection; Liver cells; Microfluidics; P450 enzyme

Indexed keywords

BIPHASIC RESPONSE; CELL RESPONSE; CYTOCHROME P450; DETECTION SYSTEM; ENZYMATIC ACTIVITIES; ENZYMATIC CONVERSIONS; FLUORESCENT SIGNALS; HEPATOMA CELLS; HIGH ACTIVITY; IN-SITU; LINEAR REGION; LIVER CELLS; MAMMALIAN CELL CULTURE; METHYLCHOLANTHRENE; MICRO FLUIDIC SYSTEM; MICRO-FLUIDIC DEVICES; NON-INVASIVE DETECTION; OPTICAL DETECTION; OPTICAL DETECTION SYSTEM; P450 ENZYME; PLATE ASSAY; PROOF OF CONCEPT; QUANTITATIVE DETECTION; REACTION PRODUCTS; REAL TIME MEASUREMENTS; REAL TIME MONITORING; RESORUFIN;

ANIMAL CELL CULTURE; CELL MEMBRANES; ENZYMES; FLUIDIC DEVICES; FLUORESCENCE; LIVER; MAMMALS; MICROFLUIDICS; TIME MEASUREMENT;

CELL CULTURE;

3 METHYLCHOLANTHRENE; CYTOCHROME P450; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; ETHOXYRESORUFIN; MATRIGEL; RESORUFIN;

ARTICLE; BIOTRANSFORMATION; CELL STRAIN HEPG2; DEVICE; ENZYME ACTIVITY; FLUORESCENCE; HUMAN; HUMAN CELL; LIVER CELL; MAMMAL CELL; MEASUREMENT; MICROFLUIDICS; MONITORING; QUANTITATIVE ANALYSIS;

CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CYTOCHROME P-450 ENZYME SYSTEM; FLUORESCENCE; HEPATOCYTES; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES; OXAZINES;

MAMMALIA;

EID: 70349306475     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22413     Document Type: Article

References (30)

1. Bambot S, Holavanahali R, Lakowicz JR, Carter GM, Rao G. 1994. Optical oxygen sensor using fluorescence lifetime measurement. Adv Exp Med Biol 361:197-205.
2. Benes M, Hudecek J, Anzenbacher P, Hof M. 2001. Coumarin 6, hypericin, resorufins, and flavins: Suitable chromophores for fluorescence correlation spectroscopy of biological molecules. Coll Czech Chem Commun 66:855-869.
3. Brown DA, MacLellan WR, Laks H, Dunn JC, Wu BM, Beygui RE. 2007. Analysis of oxygen transport in a diffusion-limited model of engineered heart tissue. Biotechnol Bioeng 97(4):962-975. (Pubitemid 47051064)
4. Camp JP, Capitano AT. 2007. Induction of zone-like liver function gradients in HepG2 cells by varying culture medium height. Biotechnol Prog 23(6):1485-1491.
5. Cheng SY, Heilman S, Wasserman M, Archer S, Shuler ML, Wu M. 2007. A hydrogel-based microfluidic device for the studies of directed cell migration. Lab Chip 7(6):763-769. (Pubitemid 46848692)
6. Coward SM, Selden C, Mantalaris A, Hodgson HJ. 2005. Proliferation rates of HepG2 cells encapsulated in alginate are increased in a microgravity environment compared with static cultures. Artif Organs 29(2):152-158. (Pubitemid 41623720)
7. Cushing MC, Anseth KS. 2007. Materials science. Hydrogel cell cultures. Science 316(5828):1133-1134.
8. De Smet K, Bruning T, Blaszkewicz M, Bolt HM, Vercruysse A, Rogiers V. 2000. Biotransformation of trichloroethylene in collagen gel sandwich cultures of rat hepatocytes. Arch Toxicol 74(10):587-592. (Pubitemid 32010387)
9. Donato MT, Gomez-Lechon MJ, Castell JV. 1993. A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates. Anal Biochem 213(1):29-33. (Pubitemid 23250612)
10. Donato MT, Jimenez N, Castell JV, Gomez-Lechon MJ. 2004. Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes. Drug Metab Dispos 32(7):699-706. (Pubitemid 38821870)
11. Garcia E, Hasenbank MS, Finlayson B, Yager P. 2007. High-throughput screening of enzyme inhibition using an inhibitor gradient generated in a microchannel. Lab Chip 7(2):249-255. (Pubitemid 46238549)
12. Ghanem A, Shuler ML. 2000. Combining cell culture analogue reactor designs and PBPK models to probe mechanisms of naphthalene toxicity. Biotechnol Prog 16(3):334-345. (Pubitemid 30369001)
13. Giepmans BN, Adams SR, Ellisman MH, Tsien RY. 2006. The fluorescent toolbox for assessing protein location and function. Science 312(5771):217-224.
14. Kelly JH. 1994. Mar 1 Permanent human hepatocyte cell line and its use in a liver assist device (LAD). US Patent 5,290,684.
15. Kelly JH, Sussman NL. 2000. A fluorescent cell-based assay for cytochrome P-450 isozyme 1A2 induction and inhibition. J Biomol Screen 5(4):249-254.
16. Kim L, Toh YC, Voldman J, Yu H. 2007. A practical guide to microfluidic perfusion culture of adherent mammalian cells. Lab Chip 7(6):681-694. (Pubitemid 46848699)
17. Mufti NA, Shuler ML. 1995. Induction of cytochrome P-450IA1 activity in response to sublethal stresses in microcarrier-attached Hep G2 cells. Biotechnol Prog 11(6):659-663.
18. Novak L, Neuzil P, Pipper J, Zhang Y, Lee S. 2007. An integrated fluorescence detection system for lab-on-a-chip applications. Lab Chip 7(1):27-29. (Pubitemid 44974593)
19. Oh TI, Sung JH, Tatosian DA, Shuler ML, Kim D. 2007. Real-time fluorescence detection of multiple microscale cell culture analog devices in situ. Cytometry A 71(10):857-865. (Pubitemid 47606742)
20. Pihl J, Karlsson M, Chiu DT. 2005. Microfluidic technologies in drug discovery. Drug Discov Today 10(20):1377-1383.
21. Rumsey WL, Schlosser C, Nuutinen EM, Robiolio M, Wilson DF. 1990. Cellular energetics and the oxygen dependence of respiration in cardiac myocytes isolated from adult rat. J Biol Chem 265(26):15392-15402. (Pubitemid 20310928)
22. Sin A, Chin KC, Jamil MF, Kostov Y, Rao G, Shuler ML. 2004. The design and fabrication of three-chamber microscale cell culture analog devices with integrated dissolved oxygen sensors. Biotechnol Prog 20(1):338-345. (Pubitemid 38204206)
23. Sivaraman A, Leach JK, Townsend S, Iida T, Hogan BJ, Stolz DB, Fry R, Samson LD, Tannenbaum SR, Griffith LG. 2005. A microscale in vitro physiological model of the liver: Predictive screens for drug metabolism and enzyme induction. Curr Drug Metab 6(6):569-591. (Pubitemid 41672495)
24. Stresser DM, Turner SD, Blanchard AP, Miller VP, Crespi CL. 2002. Cytochrome P450 fluorometric substrates: Identification of isoform-selective probes for rat CYP2D2 and human CYP3A4. Drug Metab Dispos 30(7):845-852.
25. Sung JH, Shuler ML. 2009. A micro cell culture analog (micro CCA) with 3-D hydrogel culture of multiple cell lines to assess metabolism-dependent cytotoxicity of anti-cancer drugs. Lab Chip 9(10):1385-1394.
26. Sweeney LM, Shuler ML, Babish JG, Ghanem A. 1995. A cell culture analogue of rodent physiology: Application to naphthalene toxicology. Toxicol In Vitro 9(3):307-316.
27. Tatosian DA, Shuler ML, Kim D. 2005. Portable in situ fluorescence cytometry of microscale cell-based assays. Opt Lett 30(13):1689-1691. (Pubitemid 40972042)
28. Viravaidya K, Sin A, Shuler ML. 2004. Development of a microscale cell culture analog to probe naphthalene toxicity. Biotechnol Prog 20(1):316-323. (Pubitemid 38204204)
29. Wilkening S, Stahl F, Bader A. 2003. Comparison of primary human hepatocytes and hepatoma cell line Hepg2 with regard to their biotransformation properties. Drug Metab Dispos 31(8):1035-1042. (Pubitemid 36975674)
30. Yu H, Alexander CM, Beebe DJ. 2007. A plate reader-compatible microchannel array for cell biology assays. Lab Chip 7(3):388-391. (Pubitemid 46348308)