-
2
-
-
1842563953
-
-
in, edited by X. F. Wang and B. Herman (Wiley-Interscience, New York), Cha
-
R. M. Clegg, in Fluorescence Imaging Spectroscopy and Microscopy, edited by, X. F. Wang, and, B. Herman, (Wiley-Interscience, New York, 1996), Chap., pp. 179-252.
-
(1996)
Fluorescence Imaging Spectroscopy and Microscopy
, pp. 179-252
-
-
Clegg, R.M.1
-
4
-
-
0033548686
-
Fluorescence spectroscopy of single biomolecules
-
DOI 10.1126/science.283.5408.1676
-
S. Weiss, Science 0036-8075 283, 1676 (1999). 10.1126/science.283.5408. 1676 (Pubitemid 29143793)
-
(1999)
Science
, vol.283
, Issue.5408
, pp. 1676-1683
-
-
Weiss, S.1
-
5
-
-
0029987587
-
Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor
-
DOI 10.1073/pnas.93.13.6264
-
T. Ha, T. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss, Proc. Natl. Acad. Sci. U.S.A. 0027-8424 93, 6264 (1996). 10.1073/pnas.93.13.6264 (Pubitemid 26230293)
-
(1996)
Proceedings of the National Academy of Sciences of the United States of America
, vol.93
, Issue.13
, pp. 6264-6268
-
-
Ha, T.1
Enderle, Th.2
Ogletree, D.F.3
Chemla, D.S.4
Selvin, P.R.5
Weiss, S.6
-
6
-
-
0033616580
-
Single-pair fluorescence resonance energy transfer on freely diffusing molecules: Observation of Forster distance dependence and subpopulations
-
DOI 10.1073/pnas.96.7.3670
-
A. A. Deniz, M. Dahan, J. R. Grunwell, T. J. Ha, A. E. Faulhaber, D. S. Chemla, S. Weiss, and P. G. Schultz, Proc. Natl. Acad. Sci. U.S.A. 0027-8424 96, 3670 (1999). 10.1073/pnas.96.7.3670 (Pubitemid 29168968)
-
(1999)
Proceedings of the National Academy of Sciences of the United States of America
, vol.96
, Issue.7
, pp. 3670-3675
-
-
Deniz, A.A.1
Dahan, M.2
Grunwell, J.R.3
Ha, T.4
Faulhaber, A.E.5
Chemla, D.S.6
Weiss, S.7
Schultz, P.G.8
-
7
-
-
0037126290
-
Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy
-
DOI 10.1038/nature01060
-
B. Schuler, E. A. Lipman, and W. A. Eaton, Nature (London) 0028-0836 419, 743 (2002). 10.1038/nature01060 (Pubitemid 35177962)
-
(2002)
Nature
, vol.419
, Issue.6908
, pp. 743-747
-
-
Schuler, B.1
Lipman, E.A.2
Eaton, W.A.3
-
8
-
-
0041321045
-
Single-molecule measurement of protein folding kinetics
-
DOI 10.1126/science.1085399
-
E. A. Lipman, B. Schuler, O. Bakajin, and W. A. Eaton, Science 0036-8075 301, 1233 (2003). 10.1126/science.1085399 (Pubitemid 37052211)
-
(2003)
Science
, vol.301
, Issue.5637
, pp. 1233-1235
-
-
Lipman, E.A.1
Schuler, B.2
Bakajin, O.3
Eaton, W.A.4
-
9
-
-
27744552000
-
Single-molecule FRET studies of important intermediates in the nucleocapsid-protein-chaperoned minus-strand transfer step in HIV-1 reverse transcription
-
DOI 10.1529/biophysj.105.065326
-
H. W. Liu, G. Cosa, C. F. Landes, Y. N. Zeng, B. J. Kovaleski, D. G. Mullen, G. Barany, K. Musier-Forsyth, and P. F. Barbara, Biophys. J. 0006-3495 89, 3470 (2005). 10.1529/biophysj.105.065326 (Pubitemid 41636101)
-
(2005)
Biophysical Journal
, vol.89
, Issue.5
, pp. 3470-3479
-
-
Liu, H.-W.1
Cosa, G.2
Landes, C.F.3
Zeng, Y.4
Kovaleski, B.J.5
Mullen, D.G.6
Barany, G.7
Musier-Forsyth, K.8
Barbara, P.F.9
-
10
-
-
33646416480
-
-
1089-5639,. 10.1021/jp055886d
-
L. P. Watkins, H. Y. Chang, and H. Yang, J. Phys. Chem. A 1089-5639 110, 5191 (2006). 10.1021/jp055886d
-
(2006)
J. Phys. Chem. A
, vol.110
, pp. 5191
-
-
Watkins, L.P.1
Chang, H.Y.2
Yang, H.3
-
12
-
-
22144492905
-
Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation
-
DOI 10.1529/biophysj.104.054114
-
N. K. Lee, A. N. Kapanidis, Y. Wang, X. Michalet, J. Mukhopadhyay, R. H. Ebright, and S. Weiss, Biophys. J. 0006-3495 88, 2939 (2005). 10.1529/biophysj.104.054114 (Pubitemid 40976158)
-
(2005)
Biophysical Journal
, vol.88
, Issue.4
, pp. 2939-2953
-
-
Lee, N.K.1
Kapanidis, A.N.2
Wang, Y.3
Michalet, X.4
Mukhopadhyay, J.5
Ebright, R.H.6
Weiss, S.7
-
13
-
-
14544283620
-
Polyproline and the "spectroscopic ruler" revisited with single-molecule flourescence
-
DOI 10.1073/pnas.0408164102
-
B. Schuler, E. A. Lipman, P. J. Steinbach, M. Kumke, and W. A. Eaton, Proc. Natl. Acad. Sci. U.S.A. 0027-8424 102, 2754 (2005). 10.1073/pnas. 0408164102 (Pubitemid 40299865)
-
(2005)
Proceedings of the National Academy of Sciences of the United States of America
, vol.102
, Issue.8
, pp. 2754-2759
-
-
Schuler, B.1
Lipman, E.A.2
Steinbach, P.J.3
Kumkell, M.4
Eaton, W.A.5
-
14
-
-
34547823631
-
A 10-A spectroscopic ruler applied to short polyprolines
-
DOI 10.1021/ja072178s
-
H. Sahoo, D. Roccatano, A. Hennig, and W. M. Nau, J. Am. Chem. Soc. 0002-7863 129, 9762 (2007). 10.1021/ja072178s (Pubitemid 47237490)
-
(2007)
Journal of the American Chemical Society
, vol.129
, Issue.31
, pp. 9762-9772
-
-
Sahoo, H.1
Roccatano, D.2
Hennig, A.3
Nau, W.M.4
-
15
-
-
84981779372
-
-
10.1002/and19484370105
-
T. Förster, Annalen Der Physik 2, 55 (1948). 10.1002/andp. 19484370105
-
(1948)
Annalen der Physik
, vol.2
, pp. 55
-
-
Förster, T.1
-
16
-
-
0037663777
-
-
1089-5647,. 10.1021/jp034285o
-
Z. S. Wang and D. E. Makarov, J. Phys. Chem. B 1089-5647 107, 5617 (2003). 10.1021/jp034285o
-
(2003)
J. Phys. Chem. B
, vol.107
, pp. 5617
-
-
Wang, Z.S.1
Makarov, D.E.2
-
17
-
-
34548096938
-
Theory of photon statistics in single-molecule Förster resonance energy transfer
-
DOI 10.1063/1.1812746, 014707
-
I. Gopich and A. Szabo, J. Chem. Phys. 0021-9606 122, 014707 (2005). 10.1063/1.1812746 (Pubitemid 41047794)
-
(2005)
Journal of Chemical Physics
, vol.122
, Issue.1
, pp. 1-18
-
-
Gopich, I.1
Szabo, A.2
-
18
-
-
36249028655
-
Single-molecule FRET with diffusion and conformational dynamics
-
DOI 10.1021/jp075255e
-
I. V. Gopich and A. Szabo, J. Phys. Chem. B 1089-5647 111, 12925 (2007). 10.1021/jp075255e (Pubitemid 350136484)
-
(2007)
Journal of Physical Chemistry B
, vol.111
, Issue.44
, pp. 12925-12932
-
-
Gopich, I.V.1
Szabo, A.2
-
20
-
-
70349299617
-
-
A transforms the rate for occurrence of this transition into the rate of acceptor fluorescence by counting only the fraction of A excitations that eventually fluoresce. This simple correction requires that the intramolecular rate constants of the acceptor do not depend on the donor's state, a condition mandated by property 2 above.
-
A transforms the rate for occurrence of this transition into the rate of acceptor fluorescence by counting only the fraction of A excitations that eventually fluoresce. This simple correction requires that the intramolecular rate constants of the acceptor do not depend on the donor's state, a condition mandated by property 2 above.
-
-
-
-
21
-
-
70349270366
-
-
The rate-limiting stefor emission by either D or A is the excitation transition, which occurs with rate (The system is almost always in the D A ground state because of the weak excitation conditions). The factors multiplying in the emission rates reflect the probabilities that a single excitation beginning in D A ultimately yields either a donor or acceptor photon.
-
The rate-limiting step for emission by either D or A is the excitation transition, which occurs with rate (The system is almost always in the D A ground state because of the weak excitation conditions). The factors multiplying in the emission rates reflect the probabilities that a single excitation beginning in D A ultimately yields either a donor or acceptor photon.
-
-
-
-
22
-
-
0039025943
-
-
0022-3654,. 10.1021/j100036a009
-
J. Widengren, U. Mets, and R. Rigler, J. Phys. Chem. 0022-3654 99, 13368 (1995). 10.1021/j100036a009
-
(1995)
J. Phys. Chem.
, vol.99
, pp. 13368
-
-
Widengren, J.1
Mets, U.2
Rigler, R.3
-
23
-
-
34247502146
-
Photobleaching pathways in single-molecule FRET experiments
-
DOI 10.1021/ja068002s
-
X. X. Kong, E. Nir, K. Hamadani, and S. Weiss, J. Am. Chem. Soc. 0002-7863 129, 4643 (2007). 10.1021/ja068002s (Pubitemid 46648763)
-
(2007)
Journal of the American Chemical Society
, vol.129
, Issue.15
, pp. 4643-4654
-
-
Kong, X.1
Nir, E.2
Hamadani, K.3
Weiss, S.4
-
24
-
-
70349298088
-
-
2 (Alexa Fluor 488 at 493 nm).
-
2 (Alexa Fluor 488 at 493 nm).
-
-
-
-
25
-
-
0000890528
-
-
in, 2nd ed., edited by J. B. Pawley (Springer, New York), Cha
-
H. E. Keller, in Handbook of Biological Confocal Microscopy, 2nd ed., edited by, J. B. Pawley, (Springer, New York, 1995), Chap., pp. 111-126.
-
(1995)
Handbook of Biological Confocal Microscopy
, pp. 111-126
-
-
Keller, H.E.1
-
26
-
-
33645429016
-
-
0022-3654,. 10.1021/j100540a008
-
D. T. Gillespie, J. Phys. Chem. 0022-3654 81, 2340 (1977). 10.1021/j100540a008
-
(1977)
J. Phys. Chem.
, vol.81
, pp. 2340
-
-
Gillespie, D.T.1
-
27
-
-
37649015345
-
-
0027-8424,. 10.1073/pnas.0709567104
-
R. B. Best, K. A. Merchant, I. V. Gopich, B. Schuler, A. Bax, and W. A. Eaton, Proc. Natl. Acad. Sci. U.S.A. 0027-8424 104, 18964 (2007). 10.1073/pnas.0709567104
-
(2007)
Proc. Natl. Acad. Sci. U.S.A.
, vol.104
, pp. 18964
-
-
Best, R.B.1
Merchant, K.A.2
Gopich, I.V.3
Schuler, B.4
Bax, A.5
Eaton, W.A.6
-
28
-
-
70349298087
-
-
T. Competing transfer mechanisms, breakdown of the dipole approximation, and/or technical details related to photon detection may also lead to deviations between experimentally measured transfer efficiencies and our theory. Acceptor photobleaching can also reduce E if data from donor-only sample molecules is not eliminated.
-
T. Competing transfer mechanisms, breakdown of the dipole approximation, and/or technical details related to photon detection may also lead to deviations between experimentally measured transfer efficiencies and our theory. Acceptor photobleaching can also reduce E if data from donor-only sample molecules is not eliminated.
-
-
-
-
29
-
-
0018464261
-
-
0006-3495,. 10.1016/S0006-3495(79)85243-1
-
R. E. Dale, J. Eisinger, and W. E. Blumberg, Biophys. J. 0006-3495 26, 161 (1979). 10.1016/S0006-3495(79)85243-1
-
(1979)
Biophys. J.
, vol.26
, pp. 161
-
-
Dale, R.E.1
Eisinger, J.2
Blumberg, W.E.3
-
30
-
-
33847254454
-
Ultrafast dynamics of protein collapse from single-molecule photon statistics
-
DOI 10.1073/pnas.0611093104
-
D. Nettels, I. V. Gopich, A. Hoffmann, and B. Schuler, Proc. Natl. Acad. Sci. U.S.A. 0027-8424 104, 2655 (2007). 10.1073/pnas.0611093104 (Pubitemid 46327935)
-
(2007)
Proceedings of the National Academy of Sciences of the United States of America
, vol.104
, Issue.8
, pp. 2655-2660
-
-
Nettels, D.1
Gopich, I.V.2
Hoffmann, A.3
Schuler, B.4
-
31
-
-
23744452985
-
Energy dissipation in multichromophoric single dendrimers
-
DOI 10.1021/ar040126r
-
F. C. De Schryver, T. Vosch, M. Cotlet, M. Van der Auweraer, K. Mullen, and J. Hofkens, Acc. Chem. Res. 0001-4842 38, 514 (2005). 10.1021/ar040126r (Pubitemid 41128403)
-
(2005)
Accounts of Chemical Research
, vol.38
, Issue.7
, pp. 514-522
-
-
De Schryver, F.C.1
Vosch, T.2
Cotlet, M.3
Van Der Auweraer, M.4
Mullen, K.5
Hofkens, J.6
-
32
-
-
70349279687
-
-
Multiple excitation transfer pathways are needed to describe annihilation, violating our assumption 2.
-
Multiple excitation transfer pathways are needed to describe annihilation, violating our assumption 2.
-
-
-
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