Gene trap: Knockout on the fast lane

Methods in Molecular Biology

Volumn 561, Issue , 2009, Pages 145-159

  Ullrich, Melanie ^a   Schuh, Kai ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

Galactosidase; Geo; Embryonic stem cells; Gene disruption; Gene trap; Mouse genome; Promoter activity; X gal staining

Indexed keywords

ANIMAL; ANIMAL EMBRYO; ARTICLE; CYTOLOGY; EMBRYONIC STEM CELL; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; METHODOLOGY; MOUSE; MOUSE MUTANT; PHYSIOLOGY; POLYMERASE CHAIN REACTION; PRENATAL DEVELOPMENT;

ANIMALS; EMBRYO, MAMMALIAN; EMBRYONIC STEM CELLS; GENETIC VECTORS; MICE; MICE, KNOCKOUT; MUTAGENESIS, INSERTIONAL; POLYMERASE CHAIN REACTION;

EID: 70349248116     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-019-9_10     Document Type: Article

References (13)

1. Lander, E. S., Linton, L. M., Birren, B., Nusbaum, C., Zody, M. C., Baldwin, J., Devon, K., Dewar, K., Doyle, M., FitzHugh, W. et al. (2001). Initial sequencing and analysis of the human genome. Nature, 409, 860-921
2. Venter, J. C., Adams, M. D., Myers, E. W., Li, P. W., Mural, R. J., Sutton, G. G., Smith, H. O., Yandell, M., Evans, C. A., Holt, R. A. et al. (2001). The sequence of the human genome. Science, 291, 1304-1351
3. Waterston, R. H., Lindblad-Toh, K., Birney, E., Rogers, J., Abril, J. F., Agarwal, P., Agarwala, R., Ainscough, R., Alexandersson, M., An, P. et al. (2002). Initial sequencing and comparative analysis of the mouse genome. Nature, 420, 520-562
4. Friedrich, G. and Soriano, P. (1991). Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice. Genes Dev, 5, 1513-1523
5. Gossler, A., Joyner, A. L., Rossant, J. and Skarnes, W. C. (1989). Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science, 244, 463-465
6. Skarnes, W. C., Auerbach, B. A. and Joyner, A. L. (1992). A gene trap approach in mouse embryonic stem cells: The lacZ reported is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes Dev, 6, 903-918
7. Wurst, W., Rossant, J., Prideaux, V., Kownacka, M., Joyner, A., Hill, D. P., Guillemot, F., Gasca, S., Cado, D., Auerbach, A. et al. (1995). A large-scale gene-trap screen for insertional mutations in developmentally regulated genes in mice. Genetics, 139, 889-899
8. Van Buul, P. P. and Leonard, A. (1974) Translocations in mouse spermatogonia after exposure to unequally fractionated doses of x-rays. Mutat Res, 25, 361-365
9. Russell, L. B., Hunsicker, P. R., Cacheiro, N. L., Bangham, J. W., Russell, W. L. and Shelby, M. D. (1989). Chlorambucil effectively induces deletion mutations in mouse germ cells. Proc Natl Acad Sci U S A, 86, 3704-3708
10. Russell, W. L., Kelly, E. M., Hunsicker, P. R., Bangham, J. W., Maddux, S. C. and Phipps, E. L. (1979). Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse. Proc Natl Acad Sci U S A, 76, 5818-5819
11. Bundschu, K., Gattenlohner, S., Knobeloch, K. P., Walter, U. and Schuh, K. (2006). Tissue-specific Spred-2 promoter activity characterized by a gene trap approach. Gene Expr Patterns, 6, 247-255
12. Bundschu, K., Knobeloch, K. P., Ullrich, M., Schinke, T., Amling, M., Engelhardt, C. M., Renne, T., Walter, U. and Schuh, K. (2005). Gene disruption of Spred-2 causes dwarfism. J Biol Chem, 280, 28572-28580
13. Chomczynski, P. and Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem, 162, 156-159